Nanosafety evaluation through feces: A comparison between selenium nanoparticles and selenite in rats
Source abstract
Abstract
Abstract Nanosafety evaluation is necessary not only for nanomaterials research and development but also as a cornerstone for legal regulation. In this study, we proposed a non-invasive protocol for nanosafety evaluation through feces using metagenomics and metabolomics together with metallomics. Male SD rats were orally exposed to equal amounts of Se (Se0NPs, 3.14 or Na2SeO3, 6.28 μg/kg bw) and were sacrificed after 24 h. 16S rRNA analysis and LC–MS were used to study the impact of different forms of Se on intestinal microbiota and metabolites in fecal samples. ICP-MS and synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption spectroscopy (XAS) were used to study the concentration and transformation of Se in gastrointestinal track and feces. It was found that Se0NPs brought less alternation to the composition of intestinal microbiota and metabolites related to inflammation, immunity and gut-brain axis related responses than Na2SeO3 did. Besides, the absorbed Se0NPs could be more efficiently converted to organic Se which explained the comparable bioavailability to Na2SeO3. In all, the proposed protocol combined with metagenomics, metabolomics and metallomics in this study can be used for nanosafety evaluation in a non-invasive manner and this may also be used for the screening of the nanosafety of other nanomaterials.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Selenium in Biological Systems · Heavy Metal Exposure and Toxicity · Trace Elements in Health
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (51)
Show source-provided references
1. Differential Pd-nanocrystal facets demonstrate distinct antibacterial activity against gram-positive and gram-negative bacteria10.1038/s41467-017-02502-3 ↗
2. Poly(vinylpyrollidone) and selenocysteine modified Bi2Se3 nanoparticles enhance radiotherapy efficacy in tumor and promote radioprotection in normal tissues10.1002/adma.201701268 ↗
3. Zhao · 2007
4. Advanced nuclear analytical and related techniques for the growing challenges in nanotoxicology10.1039/c3cs60111k ↗
5. Selenium and human health10.1016/S0140-6736(11)61452-9 ↗
6. Brain, immune system and selenium: A starting point for a new diagnostic marker for Alzheimer’s disease?10.1177/1757913918778707 ↗
7. Fast quantification and speciation of selenium in dietary supplements through handheL.d XRF and synchrotron radiation XAS10.46770/AS.2020.03.005 ↗
8. Biological effects of a nano red elemental selenium10.1002/biof.5520150103 ↗
9. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice10.1016/j.freeradbiomed.2007.02.013 ↗
10. A subchronic toxicity study of elemental nano-Se in sprague dawley rats10.1016/j.lfs.2004.09.026 ↗
11. Influence of dietary nano elemental selenium on growth performance, tissue selenium distritution, meat quality, and glutathione peroxidase activity in Guangxi Yellow chicken10.3382/ps.2010-00977 ↗
12. Comparative effects of nano elemental selenium and sodium selenite on selenium retention in broiler chickens10.1016/j.anifeedsci.2012.08.010 ↗
13. Effect of sodium selenite, Se-yeast and nano-elemental selenium on growth performance, Se concentration and antioxidant status in growing male goats10.1016/j.smallrumres.2010.11.005 ↗
14. GE11 peptide conjugated selenium nanoparticles for EGFR targeted oridonin delivery to achieve enhanced anticancer efficacy by inhibiting EGFR-mediated PI3K/AKT and Ras/Raf/MEK/ERK pathways10.1080/10717544.2017.1386729 ↗
15. Selenium nanoparticles as an efficient nanomedicine for the therapy of Huntington’s Disease10.1021/acsami.9b12319 ↗
16. The nano-intestine interaction: understanding the location-oriented effects of engineered nanomaterials in the intestine10.1002/smll.201907665 ↗
17. Ghrelin modulates the activity and synaptic input organization of midbrain dopamine neurons while promoting appetite10.1172/JCI29867 ↗
18. The gut microbiome from patients with schizophrenia modulates the glutamine-GABA cycle and schizophenia relevant behaviors in mice10.1126/sciadv.aau8317 ↗
19. Impact of extraction processes on prebiotic potential of the brown seaweed Ecklonia radiata by in vitro human gut bacteria fermentation10.1016/j.jff.2016.04.016 ↗
20. Immobilization of mercury by nano-elemental selenium and the underlying mechanisms in hydroponic-cultured garlic plant10.1039/C9EN01294J ↗
21. Comparison of the fecal microbiota of dholes high-throughput Illumina sequencing of the V3-V4 region of the 16S rRNA gene10.1007/s00253-015-7257-y ↗
22. Toward ‘omic’ scale metabolite profiling: a dual separation-mass spectrometry approach for coverage of lipid and central carbon metabolism10.1021/ac401140h ↗
23. Autonomous metabolomics for rapid metabolite identification in global profiling10.1021/ac5025649 ↗
24. The concentration of selenium matters: a field study on mercury accumulation in rice by selenite treatment in Qingzhen, Guizhou, China10.1007/s11104-015-2418-4 ↗
25. Hard X-ray micro-focusing beamline at SSRF
26. The progress of X-ray fluorescence computed tomography at SSRF10.1016/j.nimb.2013.04.045 ↗
27. Thiophenol adsorption on Ni(100) studied by S K-edge SEXAFS and XANES10.1016/0039-6028(91)90557-9 ↗
28. The KEGG database10.1002/0470857897.ch8 ↗
29. Absorption, distribution, metabolism and excretion of selenium following oral administration of elemental selenium nanoparticles or selenite in rats10.1039/c3mt00309d ↗
30. Selenium in the environment10.1016/0048-9697(81)90108-X ↗
31. Characterization of antimicrobial-producing beneficial bacteria isolated from huanglongbing Escape Citrus trees10.3389/fmicb.2017.02415 ↗
32. Oxidative stress in bacteria and protein damage by reactive oxygen species
33. Reductions in intestinal Clostridiales precede the development of nosocomial Clostridium difficile infection10.1186/2049-2618-1-18 ↗
34. Models of environmentally induced neurological disease: epidemiology and etiology of amyotrophic lateral sclerosis and parkinsonism-dementia in the Western Pacific10.1007/BF01734063 ↗
35. Fractionation of selenium isotopes during bacterial respiratory reduction of selenium oxyanions10.1016/S0016-7037(00)00456-7 ↗
36. Direct determination of selenium in serum by graphite-furnace atomic absorption spectrometry with deuterium background correction and a reduced palladium modifier: age-specific reference ranges10.1093/clinchem/34.4.709 ↗
37. Biogenic nano-selenium particles effectively attenuate oxidative stress-induced intestinal epithelial barrier injury by activating the Nrf2 antioxidant pathway10.2527/asasann.2017.041 ↗
38. Research progress on adenosine in central nervous system diseases10.1111/cns.13190 ↗
39. Developmental role of tryptophan hydroxylase in the nervous system10.1007/BF02700623 ↗
40. Glutamine and citrulline concentrations reflect nitric oxide synthesis in the human nervous system10.1016/j.nrl.2017.07.013 ↗
41. Extracellular vesicles in the diagnosis and treatment of central nervous system diseases10.4103/1673-5374.266908 ↗
42. Lysine methylation regulates nervous system diseases10.1016/j.npep.2019.04.004 ↗
43. Differential effects of acute and chronic hyperammonemia on signal transduction pathways associated to NMDA receptors10.1016/S0197-0186(00)00027-9 ↗
44. Lipid-metabolizing serine hydrolases in the mammalian central nervous system: endocannabinoids and beyond10.1016/j.bbalip.2018.08.007 ↗
45. α2δ-1 couples to NMDA receptors in the hypothalamus to sustain sympathetic vasomotor activity in hypertension10.1113/JP276394 ↗
46. AIDS-related central nervous system toxoplasmosis with increased 18F-fluoroethyl-L-Tyrosine amino acid PET uptake due to LAT1/2 expression of inflammatory cells10.1097/RLU.0000000000001873 ↗
47. Glutathione antioxidant system and methylmercury-induced neurotoxicity: an intriguing interplay
48. Digestive physiology of the pig symposium: detection of dietary glutamate via gut-brain axis10.2527/jas.2012-6021 ↗
49. A call for mapping the development of the microbiota-gut-brain axis during human infancy10.1017/S0140525X18002923 ↗
50. Selenium from selenium-rich spirulina is less bioavailable than selenium from Sodium selenite and selenomethionine in selenium-deficient rats10.1093/jn/131.9.2343 ↗
51. Nano-selenium and its nanomedicine applications: a critical review10.2147/IJN.S157541 ↗