Synthesis, in-vitro and in-vivo evaluation of silver sulfadiazine and cefadroxil-loaded nanofibers against Staphylococcus aureus -infected skin wounds
Source abstract
Abstract
Abstract Augmented bacterial resistance against conventional antibiotics has provoked the requisite for synthesis of alternative treatments particularly circumventing chronic infected burn wounds. Conjugated topical administration of dual antimicrobial moieties i.e. cefadroxil (CDX) and silver sulfadiazine (SSZ) was considered to facilitate the prevention of skin and soft tissue infections. The current study was aimed to fabricate and characterize (both in-vitro & in-vivo ) CDX and SSZ-loaded chitosan (CS)/poly(vinyl alcohol) (PVA) nanofibers (NFs) (CS/PNFs). The composite fibers were analyzed for in-vitro antibacterial activity against Staphylococcus aureus clinical isolates collected from the burn unit of Jinnah Hospital, Lahore, Pakistan, and for in-vivo burn wound healing capabilities on rabbit models. The fabricated NFs presented excellent antibacterial activity against S. aureus particularly F5 (zone of inhibition = 15 mm). In-vivo analysis of bioactive wound dressing released higher wound closure concerning control and marketed product (Quench). Histopathological analysis revealed the formation of dense granulation tissue, along with complete epidermal regeneration, neovascularization, reformed collagen, folliculitis, and the presence of sebaceous glands. The acquired findings confirmed the successful synthesis of CDX and SSZ-loaded CS/PNFs as a promising antibacterial wound dressing material for accelerated healing of chronically infected burn wounds through a topical co-delivery of two pharmaceutical components (CDX and SSZ).
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Wound Healing and Treatments · Electrospun Nanofibers in Biomedical Applications · Silk-based biomaterials and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmaceutics, Faculty of Pharmacy , Bahauddin Zakariya University , Multan , Pakistan
Gomal Centre of Pharmaceutical Sciences, Faculty of Pharmacy , Gomal University Dera Ismail Khan , Dera Ismail Khan , Pakistan
Department of Biology, College of Science , Princess Nourah bint Abdulrahman University , P.O. Box 84428 , Riyadh , 11671 , Saudi Arabia
Department of Biological Sciences, College of Science , University of Jeddah , Jeddah , 23445 , Saudi Arabia
Department of Pharmaceutical Sciences, Pharmacy Program , Batterjee Medical College , P.O. Box 6231 , Jeddah , 21442 , Saudi Arabia
Pharmacology Department, Faculty of Veterinary Medicine , Suez Canal University , Ismailia , 41522 , Egypt
Institute of Systems Biology (INBIOSIS) , Universiti Kebangsaan Malaysia , Bangi , 43600 , Selangor , Malaysia
Faculty of Pharmacy , MAIWP International University , Batu Caves, 68100 , Kuala Lumpur , Malaysia
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