DataVXACS Applied Nano MaterialsPaper
journal article·2025-01-24Open access

Nanoporous Graphene Integrated onto Bimodal Waveguide Biosensors for Detection of C-Reactive Protein

Bárbara Lisboa, Maria Soler, Rukmani Singh, Jesús Castro-Esteban, Diego Peña, Aitor Mugarza, Laura M. Lechuga, César Moreno

ACS Applied Nano Materials· Volume 8· Issue 3
Publisher pageOpen full text
Source abstract

Abstract

Despite the outstanding progress in photonic sensor devices, a major limitation for its application as label-free biosensors for biomedical analysis lies in the surface biofunctionalization step, that is, the reliable immobilization of the biorecognition element onto the sensor surface. Here, we report the integration of bottom-up synthesized nanoporous graphene onto bimodal waveguide interferometric biosensors as an atomically precise biofunctionalization scaffold. This combination leverages the high sensitivity of bimodal waveguide interferometers and the large functional surface area of nanoporous graphene to create highly sensitive, selective, and robust biosensors for the direct immunoassay detection of C-reactive protein (CRP), an inflammatory biomarker widely used in the clinical diagnosis of infections and sepsis. The limit of detection was determined at 3 ng/mL, which is well below the clinical cutoff levels required for the diagnostic detection of CRP in patient samples. This innovative approach holds promise for transforming diagnostics, environmental monitoring, and various fields requiring precise biomolecular detection.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Plasmonic and Surface Plasmon Research · Molecular Junctions and Nanostructures

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN, Bellaterra, 08193 Barcelona, Spain

Atomic Manipulation and Spectroscopy Group (AMS), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Bellaterra, 08193 Barcelona, Spain

Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS) and Departamento de Química Orgánica, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain

Oportunius, Galician Innovation Agency (GAIN), 15702 Santiago de Compostela, Spain

ICREA − Institució Catalana de Recerca i Estudis Avançats, 08010 Barcelona, Spain

Departamento de Ciencias de la Tierra y Física de la Materia Condensada, Universidad de Cantabria, 39005 Santander, Spain

Funding

Ministerio de Ciencia, Innovaci?n y Universidades · Agencia Estatal de Investigaci?n · European Regional Development Fund · Xunta de Galicia · NextGenerationEU · Generalitat de Catalunya · European Social Fund Plus · HORIZON EUROPE Digital, Industry and Space · Departament de Recerca i Universitats de la Generalitat de Catalunya

References (53)

Show source-provided references
1. 10.1126/science.aar200910.1126/science.aar2009 ↗
2. 10.1038/nnano.2010.810.1038/nnano.2010.8 ↗
3. 10.1002/adma.20211009910.1002/adma.202110099 ↗
4. 10.1021/jacs.3c0017310.1021/jacs.3c00173 ↗
5. 10.1002/smtd.20230076810.1002/smtd.202300768 ↗
6. 10.1021/acsnano.3c0612810.1021/acsnano.3c06128 ↗
7. 10.1016/j.nantod.2010.06.01010.1016/j.nantod.2010.06.010 ↗
8. 10.1021/jacs.5b0252310.1021/jacs.5b02523 ↗
9. 10.1038/ncomms909810.1038/ncomms9098 ↗
10. 10.1021/acsnano.0c0183710.1021/acsnano.0c01837 ↗
11. 10.1007/s12274-017-1550-210.1007/s12274-017-1550-2 ↗
12. 10.1021/acsnano.9b0816210.1021/acsnano.9b08162 ↗
13. 10.1021/jacs.0c0523510.1021/jacs.0c05235 ↗
14. 10.1021/jacs.3c1084210.1021/jacs.3c10842 ↗
15. 10.1021/acsnano.1c0432710.1021/acsnano.1c04327 ↗
16. 10.1039/D3NH00590A10.1039/D3NH00590A ↗
17. 10.1007/s00216-021-03751-410.1007/s00216-021-03751-4 ↗
18. 10.1109/JLT.2011.215073410.1109/JLT.2011.2150734 ↗
19. 10.1038/s41377-020-00460-y10.1038/s41377-020-00460-y ↗
20. 10.3390/s2412370510.3390/s24123705 ↗
21. 10.1021/acssensors.6b0016210.1021/acssensors.6b00162 ↗
22. 10.1021/acs.analchem.2c0289510.1021/acs.analchem.2c02895 ↗
23. 10.1016/j.aca.2019.05.03810.1016/j.aca.2019.05.038 ↗
24. 10.3390/diagnostics1010084510.3390/diagnostics10100845 ↗
25. 10.1039/C9AN01485C10.1039/C9AN01485C ↗
26. 10.1002/jbio.20160015410.1002/jbio.201600154 ↗
27. 10.1016/j.rio.2022.10028510.1016/j.rio.2022.100285 ↗
28. 10.1088/2515-7647/aafebb10.1088/2515-7647/aafebb ↗
29. 10.1109/JPHOT.2019.290074110.1109/JPHOT.2019.2900741 ↗
30. 10.1109/JLT.2021.311810310.1109/JLT.2021.3118103 ↗
31. 10.1002/lpor.20230050510.1002/lpor.202300505 ↗
32. 10.1063/1.243241010.1063/1.2432410 ↗
33. 10.1021/acs.chemmater.8b0309410.1021/acs.chemmater.8b03094 ↗
34. 10.1038/nature1715110.1038/nature17151 ↗
35. 10.1063/1.475243510.1063/1.4752435 ↗
36. 10.1063/1.509452310.1063/1.5094523 ↗
37. 10.3390/nano1105123010.3390/nano11051230 ↗
38. 10.1016/j.carbon.2019.03.01810.1016/j.carbon.2019.03.018 ↗
39. 10.1038/s41467-023-37436-610.1038/s41467-023-37436-6 ↗
40. 10.1007/s10762-016-0254-010.1007/s10762-016-0254-0 ↗
41. 10.1109/JLT.2021.311942310.1109/JLT.2021.3119423 ↗
42. 10.1016/j.bios.2015.04.05210.1016/j.bios.2015.04.052 ↗
43. 10.1039/C8AY00190A10.1039/C8AY00190A ↗
44. 10.3390/s1606087010.3390/s16060870 ↗
45. 10.3390/s1603028510.3390/s16030285 ↗
46. 10.1039/C6AY02972H10.1039/C6AY02972H ↗
47. 10.3390/chemosensors801001210.3390/chemosensors8010012 ↗
48. 10.1186/s13613-018-0479-210.1186/s13613-018-0479-2 ↗
49. 10.1021/la503533g10.1021/la503533g ↗
50. 10.3390/s14020223910.3390/s140202239 ↗
51. 10.1002/lpor.20140021610.1002/lpor.201400216 ↗
52. 10.1016/j.microc.2020.10528710.1016/j.microc.2020.105287 ↗
53. 10.1002/jcla.2042010.1002/jcla.20420 ↗