Immobilization of hemoglobin on MnCO 3 sphere-loaded Au nanoparticles as highly efficient sensing platform towards hydrogen peroxide
Source abstract
Abstract
Abstract In this paper, we report the synthesis of MnCO 3 −Au hybrid microspheres and their application on the electrochemical biosensing of hydrogen peroxide (H 2 O 2 ) based on the immobilization of hemoglobin (Hb). The characterization of MnCO 3 −Au microspheres revealed that an abundance of Au nanoparticles (AuNPs) has been absorbed on the surface of the spherical MnCO 3 by the electrostatic assembly. The combined unique properties of MnCO 3 −Au microspheres are beneficial for the realization of the direct electron transfer of Hb. Hb immobilized on the microspheres maintained its biological activity, showing a surface-controlled process with the heterogeneous electron transfer rate constant ( k s ) of 2.63 s −1 . The fabricated biosensor displayed an excellent performance for the electrocatalytic reduction of H 2 O 2 . The linear range for the determination of H 2 O 2 was from 0.06–40.0 μ M with a detection limit of 0.015 µ M (S/N = 3). The biosensor also exhibited high selectivity, good repeatability and long-term stability, which offers great potential for H 2 O 2 detection in real sample analysis.
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Research context
OpenAlex topics: Electrochemical sensors and biosensors · Electrochemical Analysis and Applications · Advanced biosensing and bioanalysis techniques
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References (49)
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