DataVXACS Applied Nano MaterialsPaper
journal article·2022-11-25

Carbon Dots from d-Cysteine and Citric Acid Increase the Activity of the Enzyme Laccase

Xiting Wang, KeRu Deng, Jie Wu, Yurong Ma, Xin Du, Mengling Zhang, Hui Huang, Yang Liu, Zhenhui Kang

ACS Applied Nano Materials· Volume 5· Issue 11
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Source abstract

Abstract

Chiral carbon dots (CDots) attract extensive attention due to their promising application in the biological field. Their biological effects strongly depend on their chiral structure. Laccase (Lac) is a classic multicopper-containing polyphenol oxidase. The impact of the chirality of CDots on the Lac activity has not been fully emphasized and studied yet. Here, chiral CDots are prepared by thermal polymerization from l/d-cysteine (l/d-Cys) and citric acid (CA), where the obtained twins of chiral CDots possess all the same properties such as size, charge, and elemental content, except chirality. The d-CDots (256 μg mL–1) could improve the catalytic activity of Lac (88%), while the l-CDots have no obvious effect on Lac at the same concentration. Based on the results of the Michaelis constant (Km) and circular dichroism (CD) spectra, compared with l-CDots, the superior catalytic performance of d-CDots is due to the preferable combination toward Lac, resulting in the change of the α helix structure of Lac. Our work reveals the important implications for solving the problem of limited and selective regulation of enzyme activity.
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersCatalysis & surfaces

OpenAlex topics: Carbon and Quantum Dots Applications · Electrochemical sensors and biosensors · Nanocluster Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou215123, China

School of Biology & Basic Medical Science, Soochow University, Suzhou215123, China

Macao Institute of Materials Science and Engineering (MIMSE), MUST-SUDA Joint Research Center for Advanced Functional Materials, Macau University of Science and Technology, Taipa, Macao999078, China

Funding

Collaborative Innovation Center of Suzhou Nano Science and Technology · Ministry of Science and Technology of the People's Republic of China · Natural Science Foundation of Jiangsu Province · National Natural Science Foundation of China · Higher Education Discipline Innovation Project · Ningxia Hui Autonomous Region · Guangdong Province · Suzhou Key Laboratory of Functional Nano and Soft Materials · Ningxia Autonomous Region flexible introduction of science and technology innovation team · Agricultural science and technology innovation project of Suzhou Science and Technology Development Plan

References (49)

Show source-provided references
1. 10.1038/s41477-020-0595-510.1038/s41477-020-0595-5 ↗
2. 10.1002/adma.20210011410.1002/adma.202100114 ↗
3. 10.1016/j.biotechadv.2012.02.01310.1016/j.biotechadv.2012.02.013 ↗
4. 10.1038/s41570-020-0163-610.1038/s41570-020-0163-6 ↗
5. 10.1016/j.cej.2020.12627210.1016/j.cej.2020.126272 ↗
6. 10.1016/j.jenvman.2019.01.00110.1016/j.jenvman.2019.01.001 ↗
7. 10.1016/j.chemosphere.2019.12471610.1016/j.chemosphere.2019.124716 ↗
8. 10.1016/j.trac.2018.03.01110.1016/j.trac.2018.03.011 ↗
9. 10.1038/s41565-021-01051-710.1038/s41565-021-01051-7 ↗
10. 10.1021/acscentsci.0c0130610.1021/acscentsci.0c01306 ↗
11. 10.1002/adma.20170591310.1002/adma.201705913 ↗
12. 10.1002/adma.20180483810.1002/adma.201804838 ↗
13. 10.1021/acsami.6b1225210.1021/acsami.6b12252 ↗
14. 10.1002/anie.20200290410.1002/anie.202002904 ↗
15. 10.1002/anie.20171245310.1002/anie.201712453 ↗
16. 10.1039/C7NR08335A10.1039/C7NR08335A ↗
17. 10.5185/amlett.2012.icnano.15710.5185/amlett.2012.icnano.157 ↗
18. 10.1039/C9TB01390C10.1039/C9TB01390C ↗
19. 10.1021/jz201675m10.1021/jz201675m ↗
20. 10.1002/adma.20150489110.1002/adma.201504891 ↗
21. 10.1016/j.jechem.2021.07.01210.1016/j.jechem.2021.07.012 ↗
22. 10.1002/adfm.20220364710.1002/adfm.202203647 ↗
23. 10.1002/adfm.20211333510.1002/adfm.202113335 ↗
24. 10.1021/acsami.0c0464510.1021/acsami.0c04645 ↗
25. 10.1016/j.jechem.2020.04.05110.1016/j.jechem.2020.04.051 ↗
26. 10.1021/acssuschemeng.8b0679210.1021/acssuschemeng.8b06792 ↗
27. 10.1002/adfm.20180079110.1002/adfm.201800791 ↗
28. 10.1038/s41377-022-00778-910.1038/s41377-022-00778-9 ↗
29. 10.1039/D1NR01693H10.1039/D1NR01693H ↗
30. 10.1039/C6RA12420H10.1039/C6RA12420H ↗
31. 10.1039/D1NR07236F10.1039/D1NR07236F ↗
32. 10.1016/j.jcis.2022.02.10710.1016/j.jcis.2022.02.107 ↗
33. 10.1016/j.cej.2019.12301610.1016/j.cej.2019.123016 ↗
34. 10.1021/acsnano.6b0667010.1021/acsnano.6b06670 ↗
35. 10.1016/j.apcatb.2017.02.02410.1016/j.apcatb.2017.02.024 ↗
36. 10.1016/j.apcatb.2018.08.03110.1016/j.apcatb.2018.08.031 ↗
37. 10.1021/acsami.8b1712810.1021/acsami.8b17128 ↗
38. 10.1039/C4TB01983K10.1039/C4TB01983K ↗
39. 10.1104/pp.99.3.116210.1104/pp.99.3.1162 ↗
40. 10.1038/ncomms571210.1038/ncomms5712 ↗
41. 10.1038/s41586-019-1442-610.1038/s41586-019-1442-6 ↗
42. 10.1039/C7NR02285A10.1039/C7NR02285A ↗
43. 10.1016/j.colsurfa.2016.05.02810.1016/j.colsurfa.2016.05.028 ↗
44. 10.1021/acsami.5b0238610.1021/acsami.5b02386 ↗
45. 10.1021/bi020131810.1021/bi0201318 ↗
46. 10.1016/j.jclepro.2016.03.15410.1016/j.jclepro.2016.03.154 ↗
47. 10.1016/j.bios.2012.07.06610.1016/j.bios.2012.07.066 ↗
48. 10.1002/adma.20210705310.1002/adma.202107053 ↗
49. 10.1021/acscatal.0c0375610.1021/acscatal.0c03756 ↗