journal article·2025Open access

Lauryl-NrTP6 lipopeptide self-assembled nanorods for nuclear-targeted delivery of doxorubicin

Amanda Phungula, Sofia Zuffi, Sunisa Thongsom, Paolo Di Gianvincenzo, Santiago Gimenez Reyes, Ana Beatriz Caribé dos Santos Valle, Frederico Pittella, Fernando Albericio, Beatriz G. de la Torre, Sergio E. Moya

Nanoscale· Volume 17· Issue 8

Abstract

(NrTP6) and two lauric acid chains are used to encapsulate the chemotherapeutic agent doxorubicin (DX) through a solvent-exchange protocol. LPs spontaneously form nanosized rod-like assemblies in phosphate buffer. DX is trapped in the peptide regions of the assemblies. Confocal laser scanning microscopy shows that the peptide assemblies translocate into the nucleus. Cytotoxicity studies over 72 h in A549 and HeLa cancer cell lines show less toxicity for the LP encapsulated DX than for free DX. In contrast, subtoxic doses of encapsulated DX are more effective than free DX in avoiding colony formation over 14 days, with a complete absence of colonies for the LP-encapsulated DX. The results show a more efficient and slow delivery of DX to the nucleus through LP encapsulation, paving the way for the use of lower DX doses as a chemotherapeutic agent.
Source: openalex_inverted_index · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: 2D Materials and Applications · Supramolecular Self-Assembly in Materials · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Peptide Science Laboratory, School of Chemistry and Physics, University of KwaZulu-Natal, Durban 4001, South Africa

School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban 4001, South Africa

Soft Matter Nanotechnology, Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014, Donostia-San Sebastián, Spain

Molecular Oncology Laboratory, IIS BioGipuzkoa, P° Dr Beguiristain s/n, 20014 San Sebastián, Gipuzkoa, Spain

Instituto de Fisica del Sur (IFISUR-CONICET), Av. Alem 1253, Bahia Blanca 8000, Argentina

Laboratório de Desenvolvimento de Sistemas Nanoestruturados, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, 36036-900, Juiz de Fora, MG, Brasil

CIBER-BBN and Department of Organic Chemistry, University of Barcelona, Barcelona 08001, Spain

Funding

Agencia Estatal de Investigación · Ministerio de Ciencia, Innovación y Universidades

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