journal article·2022

Visualization of intercellular cargo transfer using upconverting nanoparticles

Yeongchang Goh, Jongwoo Kim, Hye Sun Park, Taeyoung Jung, Kwan Soo Hong, Sang Hwan Nam, Yung Doug Suh, Kang Taek Lee

Nanoscale· Volume 14· Issue 38
Publisher page

Abstract

Cell-cell communication is important for cellular differentiation, organ function, and immune responses. In intercellular communication, the extracellular vesicles (EVs) play a significant role in delivering the cargo molecules such as genes, proteins, and enzymes, to regulate and control the ability of the recipient cells. In this study, the observation of intercellular cargo transfer via dual-colour imaging using upconverting nanoparticles (UCNPs) has been demonstrated. Using this technique, the intercellular transport via contact-dependent and contact-independent signaling in live HeLa cells was clearly visualized with real-time, long-term single-vesicle tracking. Furthermore, it was demonstrated that the endocytosed UCNPs can be transmitted with the encapsulation of EVs labelled with fluorescent proteins.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Nanoplatforms for cancer theranostics · Advanced biosensing and bioanalysis techniques · Extracellular vesicles in disease

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju, South Korea

Laboratory for Advanced Molecular Probing (LAMP), Korea Research Institute of Chemical Technology (KRICT), Daejeon, South Korea

Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Cheongju, South Korea

Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, Daejeon, South Korea

Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea

School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea

Funding

Korea Research Institute of Chemical Technology · National Research Foundation of Korea · Gwangju Institute of Science and Technology · Korea Basic Science Institute

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