Label-free fluorescent immunoassay for Cu 2+ ion detection based on UV degradation of immunocomplex and metal ion chelates
Source abstract
Abstract
A mouse anti Cu2+-EDTA monoclonal antibody was used to capture EDTA chelated Cu2+ ions. Then UV radiation was utilized to degrade immunocomplex and Cu2+-EDTA chelates to release free Cu2+ ions. Based on their quenching effect to the fluorescent emission of CdSe/ZnS quantum dots, the released Cu2+ ions could be detected with ideal specificity and high sensitivity.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Molecular Sensors and Ion Detection · Advanced biosensing and bioanalysis techniques · Analytical Chemistry and Sensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education)
College of Pharmaceutical Sciences
Southwest University
Chongqing 400716
China
References (34)
Show source-provided references
1. O'Neil · Anal. Chem. · 2015 · 87 · 493310.1021/acs.analchem.5b00597 ↗
2. Ji · ACS Nano · 2015 · 9 · 1092210.1021/acsnano.5b04027 ↗
3. Zhang · Anal. Chem. · 2015 · 87 · 506210.1021/acs.analchem.5b00899 ↗
4. Ilbeigi · Anal. Chem. · 2016 · 88 · 732410.1021/acs.analchem.6b01664 ↗
5. Huang · Anal. Chem. · 2015 · 87 · 689010.1021/acs.analchem.5b01362 ↗
6. Giokas · Anal. Chem. · 2004 · 76 · 130310.1021/ac0303517 ↗
7. Correia · J. Chem. Educ. · 2004 · 81 · 117410.1021/ed081p1174 ↗
8. Buffin · J. Chem. Educ. · 1999 · 76 · 167810.1021/ed076p1678 ↗
9. Pergantis · Anal. Chem. · 2012 · 84 · 645410.1021/ac300302j ↗
10. Pisonero · Anal. Chem. · 2007 · 79 · 232510.1021/ac062027s ↗
11. Gao · Anal. Chem. · 2015 · 87 · 449510.1021/acs.analchem.5b00533 ↗
12. Smolik · Anal. Chem. · 2009 · 81 · 268510.1021/ac802601d ↗
13. Sitko · Anal. Chem. · 2015 · 87 · 353610.1021/acs.analchem.5b00283 ↗
14. Uematsu · J. Phys. Chem. C · 2012 · 116 · 2090210.1021/jp306241p ↗
15. Li · Biosens. Bioelectron. · 2014 · 60 · 25210.1016/j.bios.2014.04.031 ↗
16. Li · Biosens. Bioelectron. · 2015 · 66 · 25110.1016/j.bios.2014.11.032 ↗
17. Fu · Analyst · 2015 · 140 · 667610.1039/C5AN01103E ↗
18. Mohadesi · Talanta · 2007 · 72 · 9510.1016/j.talanta.2006.09.031 ↗
19. Feier · Electrochem. Commun. · 2013 · 31 · 1310.1016/j.elecom.2013.02.025 ↗
20. Yao · Anal. Bioanal. Chem. · 2017 · 409 · 109310.1007/s00216-016-0028-5 ↗
21. Kong · Biol. Trace Elem. Res. · 2013 · 152 · 12010.1007/s12011-013-9599-6 ↗
22. Johnson · Comb. Chem. High Throughput Screening · 2003 · 6 · 24510.2174/138620703106298293 ↗
23. Fan · Anal. Chem. · 2005 · 77 · 3241
24. Xu · Analyst · 2015 · 140 · 6375
25. Ruckh · ACS Nano · 2016 · 10 · 402010.1021/acsnano.5b05396 ↗
26. Dhenadhayalan · ACS Appl. Mater. Interfaces · 2016 · 8 · 2395310.1021/acsami.6b07789 ↗
27. Jin · Anal. Chem. · 2014 · 86 · 720910.1021/ac501515f ↗
28. Han · Anal. Chem. · 2010 · 82 · 310810.1021/ac100376a ↗
29. Neupane · Anal. Chem. · 2016 · 88 · 333310.1021/acs.analchem.5b04892 ↗
30. Lei · Anal. Chem. · 2015 · 87 · 197410.1021/ac504390e ↗
31. Williamson · Cell · 2017 · 168 · 84310.1016/j.cell.2017.01.019 ↗
32. Sörensen · Trans. Inst. Met. Finish. · 2003 · 81 · 8110.1080/00202967.2003.11871511 ↗
33. Sörensen · Galvanotechnik · 2002 · 93 · 5
34. Chinese Pharmacopoeia Commission , The Pharmacopoeia of the People's Republic of China, Chinese Medical Science and Technology Press, Beijing, 2015, vol. 4