On-chip EPR spectrometry of metalloproteins using superconducting lumped element resonators
Source abstract
Abstract
(four dip-pen layers). Two reference bulk samples were pipetted from the same solution onto the remaining two resonators. The microwave transmission of the device, measured at 11 mK, shows evidence of the coupling of protein spins to the photon excitations of all resonators. In particular, the resonance broadening measured as a function of magnetic field provides the spin resonance absorption spectrum. The analysis suggests that proteins tend to self-orient on the chip. It also allows estimating the single spin to single photon coupling strength, which is around 9 Hz. This high coupling value suggests that dip-pen nanolithography gives rise to a close to optimum interface between the molecules and the chip surface. The developed methodology combines an increase in sensitivity of at least three orders of magnitude with the ability to characterize multiple samples in a single experiment, opening the door to a highly sensitive multi-analyte detection technology.
Source: openalex_inverted_index · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Electron Spin Resonance Studies · Mechanical and Optical Resonators · Molecular Junctions and Nanostructures
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, Plaza San Francisco s/n, 50009 Zaragoza, Spain
Laboratorio de Microscopias Avanzadas (LMA), Universidad de Zaragoza, Ed. I+D+i. Mariano Esquillor s/n, 50018 Zaragoza, Spain
Biofisika Institute (CSICUPV/EHU) and Department of Biochemistry and Molecular Biology, University of the Basque Country, Leioa 48940, Spain
Centro de Astrobiología, INTA-CSIC, Ctra. Torrejón-Ajalvir km.4, Torrejón de Ardoz, 28850, Madrid, Spain
IMDEA Nanociencia, Cantoblanco, 28049, Madrid, Spain
Fundación ARAID, Av. Ranillas 1-D, 50018 Zaragoza, Spain
Funding
Gobierno de Aragón · Consejo Superior de Investigaciones Científicas · Agencia Estatal de Investigación
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