A portable and versatile rGO-Co 3 O 4 -Pt nanocomposite-based electrochemical sensor for ex vivo and in vivo cardiac oxidative stress monitoring
Source abstract
Abstract
The excessive production of reactive oxygen species (ROS) disrupts redox homeostasis, contributing to the development of cardiovascular diseases. Among ROS, hydrogen peroxide (H2O2) serves as a key mediator of oxidative signaling and a critical biomarker of cellular oxidative damage due to its relative stability and signaling relevance. However, current detection strategies for ROS and H2O2 often lack the necessary sensitivity, selectivity, and real-time responsiveness, underscoring the urgent need for advanced sensing platforms to support precision cardiovascular medicine. The research introduces a ROS detection based on reduced graphene oxide-cobalt oxide-platinum (rGO-Co3O4-Pt) nanocomposites for biological sample analysis. The nanocomposite platform enables H2O2 detection down to 160 nM, with linearity up to 2.50 μM. The platform showed acceptable analytical performance in terms of sensitivity, repeatability, and selectivity, which enabled both ex vivo and in vivo H2O2 monitoring. The method was validated in doxorubicin (DOX)-induced cardiotoxicity models using HL-1 cells and C57BL/6J mice, showing strong correlation with MTT-based ROS assays. The rGO-Co3O4-Pt nanocomposite improves portable analytical devices for real-world pharmacological applications. The technology enables real-time therapeutic monitoring while optimizing individualized dosing and expanding its applications to cancer treatment and diabetes management and neurological disorders.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Electrochemical sensors and biosensors · Advanced Nanomaterials in Catalysis · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy
Sunway Centre for Electrochemical Energy and Sustainable Technology (SCEEST), School of Engineering and Technology, Sunway University, No. 5 Jalan Universiti, Bandar Sunway, 47500 Petaling Jaya, Selangor Darul Ehsan, Malaysia
Department of Applied Physics, Saveetha School of Engineering, Saveetha University (SIMATS), Chennai, India
Materials and Manufacturing Research Group, James Watt School of Engineering, University of Glasgow, Glasgow G12 8QQ, UK
Centre for Research Impact and Outcome, Chitkara University, Punjab, India
Sunway Biofunctional Molecules Discovery Centre (SBMDC), School of Medical and Life Sciences, Sunway University, Sunway City, Selangor, Malaysia
Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW, Australia
Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA
Bioelectronics Task Force, University of Naples Federico II, Via Cinthia 21, 80126 Naples, Italy
Funding
Associazione Italiana per la Ricerca sul Cancro · Horizon 2020 Framework Programme · NextGenerationEU
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