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journal article·2024

Optical detection of infectious SARS-CoV-2 virions by counting spikes

Denis S. Kudryavtsev, Vera A. Mozhaeva, Igor A. Ivanov, Andrey E. Siniavin, Alexey S. Kalmykov, Anton S. Gritchenko, Boris N. Khlebtsov, Shao-Peng Wang, Bin Kang, Victor I. Tsetlin, Victor I. Balykin, Pavel N. Melentiev

Nanoscale· Volume 16· Issue 26
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Source abstract

Abstract

Existing methods for the mass detection of viruses are limited to the registration of small amounts of a viral genome or specific protein markers. In spite of high sensitivity, the applied methods cannot distinguish between virulent viral particles and non-infectious viral particle debris. We report an approach to solve this long-standing challenge using the SARS-CoV-2 virus as an example. We show that wide-field optical microscopy with the state-of-the-art mesoscopic fluorescent labels, formed by a core-shell plasmonic nanoparticle with fluorescent dye molecules in the core-shell that are strongly coupled to the plasmonic nanoparticle, not only rapidly, i.e. in less than 20 minutes after sampling, detects SARS-CoV-2 virions directly in a patient sample without a pre-concentration step, but can also distinguish between infectious and non-infectious virus strains by counting the spikes on the lipid envelope of individual viral particles.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

Nanomaterials

OpenAlex topics: Spectroscopy Techniques in Biomedical and Chemical Research · Biosensors and Analytical Detection · SARS-CoV-2 detection and testing

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the RAS, Moscow 117997, Russia

Department of Biology and General Genetics, I.M. Sechenov First Moscow State Medical University, 119048 Moscow, Russia

N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ivanovsky Institute of Virology, Ministry of Health, Moscow, 123098, Russia

Institute of Spectroscopy RAS, Moscow, Troitsk 108840, Russia

Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Saratov, 410049, Russia

State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China

Higher School of Economics, National Research University, Moscow, 101000, Russia

Funding

Russian Academy of Sciences · Russian Science Foundation · National Natural Science Foundation of China

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