journal article·2024Open access

Multiple-unit interlocking enhances the single-stranded tiles assembly of DNA nanostructures

Xiangxiang Guan, Chenyou Zhu, Yuanchen Dong, Dongsheng Liu, Chengde Mao

Nanoscale· Volume 16· Issue 42

Abstract

Single-stranded tiles (DNA brick) assembly has provided a simple and modular tool for constructing nanostructures with the potential for numerous applications. However, in this strategy, the short-strand building blocks are susceptible to environmental fluctuations and bring about rapid dissociation during assembly, resulting in instability and prolonged annealing. Thus, developing new strategies which can enhance the stability and accelerate the assembly process of DNA bricks is important. In this study, we applied the kinetically interlocking multiple-unit (KIMU) strategy to tune the process of DNA brick assembly by adopting long DNA strands as building blocks, ranging from tens of to 1000 nucleotides. We constructed a series of DNA structures with improved stability over DNA bricks. Furthermore, the annealing process could be accelerated by increasing the number of units. Our study demonstrated that DNA assembly based on the KIMU strategy using multiple-unit DNA strands could be a promising method for constructing relatively stable DNA nanostructures.
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Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · Gold and Silver Nanoparticles Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

Engineering Research Center of Advanced Rare Earth Materials, (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

CAS Key Laboratory of Colloid Interface and Chemical Thermodynamics, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China

University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA

Funding

National Basic Research Program of China · Natural Science Foundation of Beijing Municipality · National Natural Science Foundation of China · Division of Computing and Communication Foundations

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