Nanoscopic visualization of microgel-immobilized cytochrome P450 enzymes and their local activity
Source abstract
Abstract
Microgels provide a controlled microenvironment for enzymes, protecting them from degradation while enhancing stability and activity. Their customizable and biocompatible structure allows for targeted delivery and controlled release, making them ideal for transporting and preserving enzyme function in various applications. For such applications, detailed knowledge of the distribution of enzymes and their activity within the microgels is essential. We present a combination of different localization-based super-resolution fluorescence microscopy measurements to localise single Cytochrome P450 BM3 enzymes and compare their local catalytic activity.
Source: openalex_inverted_index · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Innovative Microfluidic and Catalytic Techniques Innovation · Microfluidic and Capillary Electrophoresis Applications · Advanced Fluorescence Microscopy Techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Physical Chemistry, RWTH Aachen University, Landoltweg 2, 52074 Aachen, Germany
Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany
DWI – Leibniz-Institute for Interactive Materials, Forckenbeckstraße 50, 52074 Aachen, Germany
Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074 Aachen, Germany
Aachen Maastricht Institute for Biobased Materials (AMIBM), Maastricht University, Brightlands Chemelot Campus, Urmonderbaan 22, 6167 RD Geleen, The Netherlands
Funding
Deutsche Forschungsgemeinschaft
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