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journal article·2025

A fluorescence nanosensor based on modified sustainable silica for highly sensitive detection of the SARS-CoV-2 IgG antibody

Firda Apriyani, Shaimah Rinda Sari, Himawan Tri Bayu Murti Petrus, Marissa Angelina, Robeth V. Manurung, Ni Luh Wulan Septiani, Brian Yuliarto, S. N. Aisyiyah Jenie

Nanoscale· Volume 17· Issue 9
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Source abstract

Abstract

This study presents an innovative fluorescence nanosensor utilizing modified sustainable silica for the ultra-sensitive detection of SARS-CoV-2 IgG antibodies. The sensor employs fluorescent dye-doped silica nanoparticles (FSNPs) synthesized via the sol-gel method and functionalized with rhodamine B as a fluorescent dye. Fourier-transform infrared (FTIR) analysis confirmed the successful immobilization of anti-IgG on the FSNP surface, as evidenced by the characteristic amide I and II peaks at 1641 cm-1 and 1530 cm-1, respectively. Detection of SARS-CoV-2 IgG antibodies was achieved through the enhanced fluorescence intensity of FSNP-anti-IgG at 582 nm. Optimal detection conditions were established with a 15-minute incubation period, demonstrating a linear detection range from 10-8 to 10-2 μg mL-1 and a limit of detection (LOD) of 5.3 fg mL-1. This research highlights the potential of modified sustainable silica-based fluorescence nanosensors, particularly those utilizing FSNP-anti IgG, for advancing sensitive, rapid, and cost-effective COVID-19 diagnostics, making them a viable option for pathogen detection in resource-limited settings.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · SARS-CoV-2 detection and testing

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Master Program of Nanotechnology, Graduate School, Institut Teknologi Bandung, Ganesha 10, Bandung, West Java 40132, Indonesia

Advanced Functional Material Research Group, Faculty of Industrial Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, West Java 41032, Indonesia

Research Center for Chemistry, National Research and Innovation Agency (BRIN), Kawasan Sains dan Teknologi (KST) B. J. Habibie, Building 452, Serpong, South Tangerang, Banten 15314, Indonesia

Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Jalan Grafika No 2, Yogyakarta, 55281, Indonesia

Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Cibinong Science Center (CSC), Bogor, West Java 16911, Indonesia

BRIN and ITB Collaboration Research Center for Biosensor and Biodevices, Jl. Ganesa 10, Bandung, West Java 40132, Indonesia

Research Centre for Electronics, National Research and Innovation Agency (BRIN), Kawasan Sains dan Teknologi (KST) Samaun Samadikun, Jl. Cisitu Sangkuriang, Dago, Bandung, West Java 40135, Indonesia

Research Centre for Nanotechnology Systems, National Research and Innovation Agency (BRIN), Kawasan Sains dan Teknologi (KST) B. J. Habibie, South Tangerang 15314, Indonesia

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