Influence of viral transport media and freeze–thaw cycling on the sensitivity of qRT-PCR detection of SARS-CoV-2 nucleic acids
Source abstract
Abstract
: The choice of VTM is critically important to defining the sensitivity of COVID-19 molecular diagnostics assays and this study suggests they can impact upon the stability of the SARS-CoV-2 viral genome. This becomes increasingly important if the virus structure is destabilised before analysis, which can occur due to poor storage conditions. This study suggests that COVID-19 testing performed with glycerol-containing PBS will produce a high level of stability and sensitivity. These results are in agreement with clinical studies reported for patient-derived samples.
Source: openalex_inverted_index · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: SARS-CoV-2 detection and testing · Viral gastroenteritis research and epidemiology · SARS-CoV-2 and COVID-19 Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Chemistry and Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Republic of Ireland
Magnostics Ltd, Monkstown, Co. Dublin, Republic of Ireland
National Virus Reference Laboratory, University College Dublin, Belfield, Dublin, Republic of Ireland
International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
Funding
European Commission · Science Foundation Ireland
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