journal article·2022Open access

Anti-KIT DNA aptamer-conjugated porous silicon nanoparticles for the targeted detection of gastrointestinal stromal tumors

Sanahan Vijayakumar, Seyedmehdi H. Nasr, Jacob E. Davis, Edward Wang, Jonathan M. Zuidema, Yi-Sheng Lu, Yu-Hwa Lo, Jason K. Sicklick, Michael J. Sailor, Partha Ray

Nanoscale· Volume 14· Issue 47

Abstract

Evaluation of Gastrointestinal Stromal Tumors (GIST) during initial clinical staging, surgical intervention, and postoperative management can be challenging. Current imaging modalities (e.g., PET and CT scans) lack sensitivity and specificity. Therefore, advanced clinical imaging modalities that can provide clinically relevant images with high resolution would improve diagnosis. KIT is a tyrosine kinase receptor overexpressed on GIST. Here, the application of a specific DNA aptamer targeting KIT, decorated onto a fluorescently labeled porous silicon nanoparticle (pSiNP), is used for the in vitro & in vivo imaging of GIST. This nanoparticle platform provides high-fidelity GIST imaging with minimal cellular toxicity. An in vitro analysis shows greater than 15-fold specific KIT protein targeting compared to the free KIT aptamer, while in vivo analyses of GIST-burdened mice that had been injected intravenously (IV) with aptamer-conjugated pSiNPs show extensive nanoparticle-to-tumor signal co-localization (>90% co-localization) compared to control particles. This provides an effective platform for which aptamer-conjugated pSiNP constructs can be used for the imaging of KIT-expressing cancers or for the targeted delivery of therapeutics.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

OpenAlex topics: Silicon Nanostructures and Photoluminescence · Optical Coatings and Gratings · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Materials Science and Engineering Program, University of California, San Diego, La Jolla, California, 92093, USA

Department of Chemistry & Biochemistry, University of California, San Diego, La Jolla, California, 92093, USA

Department of Surgery, Division of Surgical Oncology, University of California, San Diego, San Diego, California, 92093, USA

Moores Cancer Center, University of California, San Diego, La Jolla, California, 92093, USA

Department of Neurosciences, University of California, San Diego, San Diego, California, 92093, USA

Department of Electrical & Computer Engineering, University of California, San Diego, La Jolla, California, 92093, USA

Department of Pharmacology, University of California, San Diego, San Diego, California, 92093, USA

Funding

University of California, San Diego · National Science Foundation · Center for Wireless Communications, University of California, San Diego · NIH Clinical Center · Natural Sciences and Engineering Research Council of Canada · National Institutes of Health

References (39)

Show source-provided references
1. Ma · Cancer Epidemiol. Biomarkers Prev. · 2015 · 24 · 29810.1158/1055-9965.EPI-14-1002 ↗
2. Kang · Cancer Res. Treat. · 2010 · 42 · 13510.4143/crt.2010.42.3.135 ↗
3. Corless · Nat. Rev. Cancer · 2011 · 11 · 86510.1038/nrc3143 ↗
4. Barnett · Clin. Oncol. · 2013 · 27 · 871
5. Metildi · Ann. Surg. Oncol. · 2013 · 20 · S69310.1245/s10434-013-3172-6 ↗
6. Tuerk · Science · 1990 · 249 · 50510.1126/science.2200121 ↗
7. Ellington · Nature · 1990 · 346 · 81810.1038/346818a0 ↗
8. Kim · J. Am. Chem. Soc. · 2016 · 138 · 1510610.1021/jacs.6b08614 ↗
9. Ray · Arch. Immunol. Ther. Exp. · 2013 · 61 · 25510.1007/s00005-013-0227-0 ↗
10. Banerjee · Mol. Cancer Ther. · 2020 · 19 · 117310.1158/1535-7163.MCT-19-0959 ↗
11. Kang · Adv. Mater. · 2020 · 32 · 190589910.1002/adma.201905899 ↗
12. Tan · Theranostics · 2017 · 7 · 486210.7150/thno.19101 ↗
13. Kim · Adv. Mater. · 2017 · 29 · 170330910.1002/adma.201703309 ↗
14. Kang · Adv. Mater. · 2018 · 30 · 180051210.1002/adma.201800512 ↗
15. Bertucci · ACS Appl. Mater. Interfaces · 2019 · 11 · 2392610.1021/acsami.9b07980 ↗
16. Kang · Adv. Mater. · 2016 · 28 · 796210.1002/adma.201600634 ↗
17. Kim · Adv. Mater. · 2019 · 31 · 190295210.1002/adma.201902952 ↗
18. M. J.Sailor , Porous Silicon in Practice: Preparation, Characterization, and Applications , Wiley-VCH , Weinheim, Germany , 2012
19. Gu · Adv. Mater. · 2012 · 24 · 398110.1002/adma.201200776 ↗
20. Chen · ACS Nano · 2013 · 7 · 902710.1021/nn403617j ↗
21. Secret · Adv. Healthcare Mater. · 2013 · 2 · 71810.1002/adhm.201200335 ↗
22. Mann · Nat. Commun. · 2016 · 7 · 1198010.1038/ncomms11980 ↗
23. B.Kim , J.-H.Park and M. J.Sailor , Adv. Mater. , 1903637
24. Zhang · ACS Appl. Mater. Interfaces · 2017 · 9 · 1003410.1021/acsami.7b02196 ↗
25. Terracciano · Molecules · 2019 · 24 · 221610.3390/molecules24122216 ↗
26. Qin · Part. Part. Syst. Charact. · 2014 · 31 · 25210.1002/ppsc.201300244 ↗
27. Banerjee · Mol. Cancer Ther. · 2021 · 20 · 203510.1158/1535-7163.MCT-20-0973 ↗
28. Sicklick · J. Transl. Med. · 2014 · 12 · 4110.1186/1479-5876-12-41 ↗
29. Yebra · Clin. Cancer Res. · 2022 · 28 · 18710.1158/1078-0432.CCR-21-2092 ↗
30. Yoon · Oncogene · 2021 · 40 · 195710.1038/s41388-021-01685-w ↗
31. Gratton · Proc. Natl. Acad. Sci. U. S. A. · 2008 · 105 · 1161310.1073/pnas.0801763105 ↗
32. Thurston · J. Clin. Invest. · 1998 · 101 · 140110.1172/JCI965 ↗
33. Billinton · Anal. Biochem. · 2001 · 291 · 17510.1006/abio.2000.5006 ↗
34. McCormack · Mol. Imaging · 2007 · 6 · 729010.2310/7290.2007.00016 ↗
35. Kim · Nat. Commun. · 2018 · 9 · 196910.1038/s41467-018-04390-7 ↗
36. Park · Nat. Mater. · 2009 · 8 · 33110.1038/nmat2398 ↗
37. Li · Adv. Sci. · 2020 · 7 · 200112910.1002/advs.202001129 ↗
38. Tanaka · Mol. Cancer Ther. · 2014 · 13 · 76710.1158/1535-7163.MCT-13-0393 ↗
39. Ligiero · Biomed. Phys. Eng. Express · 2016 · 2 · 04501710.1088/2057-1976/2/4/045017 ↗