Stoichiometric quantification of spatially dense assemblies with qPAINT
Source abstract
Abstract
Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.
Source: openalex_inverted_index · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · Advanced Fluorescence Microscopy Techniques · DNA and Nucleic Acid Chemistry
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Biotechnology and Biomolecular Sciences
The University of New South Wales
Sydney
Australia
European Molecular Biology Laboratory Australia Node for Single Molecule Science
School of Medical Sciences
Funding
Australian Research Council · Human Frontier Science Program · National Health and Medical Research Council
References (23)
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