Correlative Live-Cell, Electron Microscopy and Nanoscale Secondary Ion Mass Spectrometry Elucidates the Mechanism for the Release of Cholesterol-Rich Particles from the Plasma Membrane of Macrophages

movement, more specifically the adhesion and release of lamellipodia and filopodia. Correlative live-cell, electron microscopy, and NanoSIMS (CLEMNS) was imperative for our studies especially due to the ability to resolve an isotopic tag ([ 15 N]ALO-D4) at high resolution provided by the NanoSIMS. We are certain that this technique will be applicable in a wide range of experiments as it can be modified with isotopic labeling of different cell types, proteins, nucleotides, antibodies or possibly drugs.