Integrated Analysis of Biological Samples by Imaging Flow Cytometry

Imaging flow cytometry is a recent state of the art technology that allows the fast imaging of a high amount of cells in flow. It combines the speed and sensitivity for fluorescence intensity quantification of conventional flow cytometry with the morphological features discrimination of microscopy, for the specific location and distribution of signals within individual cells and in the whole cellular population. Cellular events were acquired using the ImageStream® (Amnis, EDM Millipore, Darmstadt, Germany) imaging flow cytometer, and the analysis of multiple image-based parameters was performed using the IDEAS software (v6.0.348, Amnis, EDM Millipore, Darmstadt, Germany). To show the versatility of the imaging flow cytometer different biological samples were run to analyze and quantify several distinctive features. The results of these experiments are described below. ... In summary, as depicted by these selected examples, imaging flow cytometry provides a high resolution multispectral cell image analysis of a high amount of events, providing fast and versatile feature analysis [1].(Figure 1)