Two-Photon Imaging of Cellular Activities in Oxygen Sensing Tissues
Source abstract
Abstract
Abstract The cellular oxygen sensing system of the body ensures appropriate adaptation of cellular functions toward hypoxia by regulating gene expression and ion channel activity. Two-photon laser microscopy is an ideal tool to study and prove the relevance of the molecular mechanisms within oxygen sensing pathways on the cellular and complex tissue or organ level. Images of hypoxia inducible factor 1 (HIF-1) subunit nuclear mobility and protein-protein interaction in living cells, of hypoxia-induced changes in membrane potential and intracellular calcium of live ex vivo carotid bodies as well as of rat kidney proximal tubulus function in vivo, will be shown.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Cancer, Hypoxia, and Metabolism · High Altitude and Hypoxia · Mitochondrial Function and Pathology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (29)
Show source-provided references
1. 10.1098/rstb.2005.176010.1098/rstb.2005.1760 ↗
2. 10.1096/fj.06-6280com10.1096/fj.06-6280com ↗
3. Unusual cytochrome a(592) with low PO2 affinity correlates as putative oxygen sensor with rat carotid body chemoreceptor discharge10.1096/fj.02-0166fje ↗
4. 10.1242/jcs.0031810.1242/jcs.00318 ↗
5. 10.1016/j.cell.2007.07.04310.1016/j.cell.2007.07.043 ↗
6. 10.1042/bj272074310.1042/bj2720743 ↗
7. 10.1016/j.cardiores.2006.04.00810.1016/j.cardiores.2006.04.008 ↗
8. 10.1073/pnas.253337410010.1073/pnas.2533374100 ↗
9. Historical perspectives of cellular oxygen sensing and responses to hypoxia10.1152/jappl.2000.88.4.1467 ↗
10. 10.1073/pnas.040026510110.1073/pnas.0400265101 ↗
11. 10.1007/BF0049007610.1007/BF00490076 ↗
12. 10.1074/jbc.270.3.123010.1074/jbc.270.3.1230 ↗
13. 10.1515/BC.2004.01710.1515/BC.2004.017 ↗
14. 10.1056/NEJMra05000210.1056/NEJMra050002 ↗
15. 10.1016/j.cardiores.2006.05.00510.1016/j.cardiores.2006.05.005 ↗
16. 10.1152/ajprenal.00396.200510.1152/ajprenal.00396.2005 ↗
17. 10.1073/pnas.97.8.437410.1073/pnas.97.8.4374 ↗
18. Autonomic nervous influences upon total flow, local flow, tissue PO2 and chemosensory activity of the cat carotid body [Proceedings]
19. 10.1038/sj.ki.500231710.1038/sj.ki.5002317 ↗
20. 10.1074/jbc.M40602620010.1074/jbc.M406026200 ↗
21. Widespread hypoxia-inducible expression of HIF-2alpha in distinct cell populations of different organs10.1096/fj.02-0445fje ↗
22. 10.1126/science.287.5454.84810.1126/science.287.5454.848 ↗
23. 10.1016/0014-5793(94)00887-610.1016/0014-5793(94)00887-6 ↗
24. 10.1038/sj.embor.740059810.1038/sj.embor.7400598 ↗
25. 10.1016/S0006-8993(98)00127-910.1016/S0006-8993(98)00127-9 ↗
26. 10.1073/pnas.96.11.625510.1073/pnas.96.11.6255 ↗
27. 10.1016/j.jneumeth.2005.06.02710.1016/j.jneumeth.2005.06.027 ↗
28. 10.1016/j.bbrc.2005.08.11010.1016/j.bbrc.2005.08.110 ↗
29. Quantitative determination of the vascular volume of the cat carotid body under normoxia, hyperoxia, hypoxia and hypercapnia (author's transl)