Application of Phase Correlation to the Montage Synthesis and Three-Dimensional Reconstruction of Large Tissue Volumes from Confocal Laser Scanning Microscopy
Source abstract
Abstract
We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce “tiles” of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated.
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Research context
OpenAlex topics: Cell Image Analysis Techniques · Optical measurement and interference techniques · Image Processing Techniques and Applications
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References (22)
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