Defective Histone H3K27 Trimethylation Modification in Embryos Derived from Heated Mouse Sperm
Source abstract
Abstract
Abstract The mouse sperm genome is resistant toin vitroheat treatment, and embryos derived from heated sperm can support full-term embryonic development, but the blastocyst rate and implantation rate are lower compared to embryos derived from fresh sperm. In the present study, the patterns of DNA methylation, histone H4K12 (ACH4K12) acetylation, H3K9 trimethylation (H3K9-TriM), and H3K27 trimethylation (H3K27-TriM) in preimplantation embryos derived from 65°C-heated sperm were investigated. Although no evident changes in global DNA methyaltion, histone H4K12 (ACH4K12) acetylation, and H3K9 trimethylation (H3K9-TriM) were found, significantly lower levels of H3K27-TriM, which was thought to be one of the reasons for low efficiency of mouse cloning, were found in the inner cell mass of heated-sperm derived blastocysts. Thus, defective modification of H3K27-TriM might contribute to compromised development of embryos derived from heated sperm.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Epigenetics and DNA Methylation · Reproductive Biology and Fertility · Reproductive System and Pregnancy
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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