Scanning Electron Microscopy as a Useful Tool for Monitoring Cell Disruption of RecombinantEscherichia coliSHuffle T7

HPV16 full-length-, HPV16-truncated-and HPV16 His-tagged L1 proteins were produced in E. coli SHuffle T7 as insoluble proteins within inclusion bodies (IBs). Recovery of active protein from IBs is a difficult task, which requires separation of IBs from other component of bacteria, as the first step