Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites
Source abstract
Abstract
BACKGROUND: The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary. Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method. OBJECTIVE: The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites. METHODS: The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days. The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy. Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay. RESULTS: The results show a porous and interconnected morphology with enhanced bioactivity. It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds. CONCLUSIONS: The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Plant-Derived Bioactive Compounds · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
, , , , , México
Universidad Tecnológica de Ciudad Juárez, , , , , México
, , , , México
References (48)
Show source-provided references
1. The development of collagen based composite scaffolds for bone regeneration10.1016/j.bioactmat.2017.08.004 ↗
2. Recent advances in bone tissue engineering scaffolds10.1016/j.tibtech.2012.07.005 ↗
3. T.R. Correia, D.R. Figueira, K.D. de Sá, S.P. Miguel, R.G. Fradique, A.G. Mendonça , 3D printed scaffolds with bactericidal activity aimed for bone tissue regeneration, International Journal of Biological Macromolecules.
4. Targeting the hypoxic response in bone tissue engineering: A balance between supply and consumption to improve bone regeneration10.1016/j.mce.2015.12.024 ↗
5. Bone reservoir: Injectable hyaluronic acid hydrogel for minimal invasive bone augmentation10.1016/j.jconrel.2011.02.003 ↗
6. A unique scaffold for bone tissue engineering: An osteogenic combination of graphene oxide–hyaluronic acid–chitosan with simvastatin10.1016/j.jiec.2016.10.029 ↗
7. Fabrication and characterization of novel nano-biocomposite scaffold of chitosan–gelatin–alginate–hydroxyapatite for bone tissue engineering10.1016/j.msec.2016.03.060 ↗
8. Designing highly structured polycaprolactone fibers using microfluidics10.1016/j.jmbbm.2016.04.005 ↗
9. New porous polycaprolactone–silica composites for bone regeneration10.1016/j.msec.2014.04.024 ↗
10. The return of a forgotten polymer—Polycaprolactone in the 21st century10.1016/j.progpolymsci.2010.04.002 ↗
11. Carbon nanofibers and carbon nanotubes in regenerative medicine10.1016/j.addr.2009.07.010 ↗
12. Determination of substitution positions in hyaluronic acid hydrogels using NMR and MS based methods10.1016/j.carbpol.2015.09.112 ↗
13. Chitosan-co-Hyaluronic acid porous cryogels and their application in tissue engineering10.1016/j.ijbiomac.2017.05.067 ↗
14. Emerging roles of hyaluronic acid bioscaffolds in tissue engineering and regenerative medicine10.1016/j.ijbiomac.2016.02.032 ↗
15. Hyaluronic acid (hyaluronan): a review10.17221/1930-VETMED ↗
16. Consumer concepts in new product development of local foods: Traditional versus novel honeys10.1016/j.foodres.2013.02.030 ↗
17. A novel 3D bone-mimetic scaffold composed of collagen/MTA/MWCNT modulates cell migration and osteogenesis10.1016/j.lfs.2016.08.003 ↗
18. Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering10.1016/j.carbon.2011.04.002 ↗
19. MWCNT based matrices as a platform for adhesion and growth of cells10.1016/j.compscitech.2016.09.026 ↗
20. MWCNT reinforced bone like calcium phosphate—Hydroxyapatite composite coating developed through pulsed electrodeposition with varying amount of apatite phase and crystallinity to promote superior osteoconduction, cytocompatibility and corrosion protection performance compared to bare metallic implant surface10.1016/j.surfcoat.2017.06.073 ↗
21. Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/𝛽-Glycerophosphate scaffolds for bone tissue engineering10.1016/j.ijbiomac.2016.12.086 ↗
22. Effects of Mimosa tenuiflora on larval establishment of Haemonchus contortus in sheep10.1016/j.vetpar.2013.04.014 ↗
23. G. Erkan, K. Şengül and S. Kaya, Dyeing of white and indigo dyed cotton fabrics with Mimosa tenuiflora extract, Journal of Saudi Chemical Society (2011).
24. Evaluation of in vitro bioactivity of Chitosan/Mimosa tenuiflora composites10.1016/j.matlet.2014.01.004 ↗
25. In vitro bioactivity of chitosan/poly (d,l-lactide-co-glycolide) composites10.1016/j.matlet.2010.09.022 ↗
26. Biocomposites scaffolds for bone tissue engineering
27. In vivo evaluation of 3-dimensional polycaprolactone scaffolds for cartilage repair in rabbits10.1177/0363546509352448 ↗
28. Functionalization of polycaprolactone scaffolds with hyaluronic acid and beta-TCP facilitates migration and osteogenic differentiation of human dental pulp stem cells in vitro10.1089/ten.tea.2014.0177 ↗
29. Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro10.1002/jor.20539 ↗
30. Cell-free cartilage engineering approach using hyaluronic acid-polycaprolactone scaffolds: a study in vivo10.1177/0885328213507298 ↗
31. M.A. Sá, H.J. Ribeiro, T.M. Valverde, B.R. Sousa, P.A. Martins-Júnior, R.M. Mendes , Single-walled carbon nanotubes functionalized with sodium hyaluronate enhance bone mineralization, Brazilian Journal of Medical and Biological Research 49(2) (2016).10.1590/1414-431x20154888 ↗
32. Biodegradable chitosan/multiwalled carbon nanotube composite for bone tissue engineering10.4236/jbnb.2013.42025 ↗
33. Chitosan/MWCNT composites prepared by thermal induced phase separation10.1016/j.jallcom.2009.10.205 ↗
34. Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration10.1016/j.ijbiomac.2016.09.083 ↗
35. Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nano-hydroxyapatite/chitosan/chondroitin sulfate/hyaluronic acid for bone tissue engineering10.1016/j.colsurfb.2017.05.059 ↗
36. Porous membranes of the polycaprolactone (PCL) containing calcium silicate fibers for guided bone regeneration10.1016/j.matlet.2017.07.011 ↗
37. Production and characterization of polycaprolactone-hyaluronic acid/chitosan-zein electrospun bilayer nanofibrous membrane for tissue regeneration10.1016/j.ijbiomac.2016.09.080 ↗
38. The correlation of pore morphology, interconnectivity and physical properties of 3D ceramic scaffolds with bone ingrowth10.1016/j.biomaterials.2008.10.056 ↗
39. A novel layer-structured scaffold with large pore sizes suitable for 3D cell culture prepared by near-field electrospinning10.1016/j.msec.2017.12.016 ↗
40. Preparation and characterization of composites based on the blends of collagen, chitosan and hyaluronic acid with nano-hydroxyapatite10.1016/j.ijbiomac.2017.03.196 ↗
41. Chitosan/poly(DL,lactide-co-glycolide) scaffolds for tissue engineering10.1007/s10856-012-4762-8 ↗
42. Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties10.1016/j.carbpol.2018.07.061 ↗
43. Electrospun chitosan/polycaprolactone-hyaluronic acid bilayered scaffold for potential wound healing applications10.1016/j.ijbiomac.2018.05.099 ↗
44. Carbon nanotubes: their potential and pitfalls for bone tissue regeneration and engineering10.1016/j.nano.2013.06.001 ↗
45. Biocompatibility of chitosan/Mimosa tenuiflora scaffolds for tissue engineering10.1016/j.jallcom.2015.01.034 ↗
46. A mineralogical perspective on the apatite in bone10.1016/j.msec.2005.01.008 ↗
47. Improved properties of hydroxyapatite–carbon nanotube biocomposite: Mechanical, in vitro bioactivity and biological studies10.1016/j.ceramint.2013.10.158 ↗
48. Bio-inspired hybrid scaffold of zinc oxide-functionalized multi-wall carbon nanotubes reinforced polyurethane nanofibers for bone tissue engineering10.1016/j.matdes.2017.07.049 ↗