3D printed 13-93B3 borate bioactive glass/hydroxypropyl methyl cellulose/gelatin scaffolds with cerium oxide submicrometric particles for potential bone regeneration
Source abstract
Abstract
Abstract In this study, a novel hydroxypropyl methyl cellulose (HPMC)/gelatin composite scaffold was prepared by incorporating 13–93B3 borate bioactive glass (BBG) microparticles and cerium oxide (CeO₂) submicrometric particles as a discrete phase, enabling higher ceria loadings without disrupting the bioactive glass chemistry. Composite hydrogel inks containing 5 wt% BBG microparticles and up to 20 wt% submicrometric CeO 2 particles were successfully extrusion-printed into porous scaffolds with interconnected pore architecture. CeO 2 incorporation preserved printability and mechanical strength while significantly enhancing scaffold deformation ability. Degradation behavior was tunable, with BBG microparticles reducing swelling and CeO 2 submicrometric particles modulating water uptake and pH evolution. BBG microparticles and CeO 2 submicrometric particles synergistically promoted apatite formation following 7 d of SBF incubation. In vitro studies using MC3T3-E1 pre-osteoblasts confirmed high cytocompatibility and Alizarin red study showed enhanced mineralization in CeO 2 -containing scaffolds. Additionally, BBG and CeO 2 incorporated scaffolds exhibited strong antibacterial activity against S. aureus and E. coli . Overall, this multifunctional scaffold platform demonstrates promise for bone tissue engineering applications.
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Research context
OpenAlex topics: Bone Tissue Engineering Materials · Advanced Nanomaterials in Catalysis · Aerogels and thermal insulation
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Bogazici University
Marmara University
Şişecam Science Technology and Design Center
Experimental Medicine Research and Application Center Validebag Research Park Istanbul
Bogazici University Center for Targeted Therapy Technologies
Institute of Biomaterials, University of Erlangen-Nuremberg
Funding
TUSEB (the Health Institutes of Türkiye · Bilimsel AraütÜrma Projeleri Birimi, Boğaziçi üniversitesi · Bilimsel Araştırma Projeleri, Boğaziçi üniversitesi
References (77)
Show source-provided references
1. Advances in 3D-printed scaffold technologies for bone defect repair: materials, biomechanics, and clinical prospects10.1186/s12938-025-01381-w ↗
2. Recent trends in bone tissue engineering: a review of materials, methods, and structures10.1088/1748-605X/ad407d ↗
3. 3D printing of bone tissue engineering scaffolds10.1016/j.bioactmat.2020.01.004 ↗
4. 3D printing of gummy drug formulations composed of gelatin and an HPMC-based hydrogel for pediatric use10.1016/j.ijpharm.2020.120118 ↗
5. Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes10.1016/j.colsurfb.2017.09.059 ↗
6. Hydroxypropylmethyl cellulose (HPMC) crosslinked keratin/hydroxyapatite (HA) scaffold fabrication, characterization and in vitro biocompatibility assessment as a bone graft for alveolar bone regeneration10.1016/j.heliyon.2021.e08294 ↗
7. Cellulose-based composite scaffolds for bone tissue engineering and localized drug delivery10.1016/j.bioactmat.2022.05.018 ↗
8. Enzymatically functionalized RGD-gelatin scaffolds that recruit host mesenchymal stem cells in vivo and promote bone regeneration10.1016/j.jcis.2021.12.091 ↗
9. 3D printing of calcium phosphate/calcium sulfate with alginate/cellulose-based scaffolds for bone regeneration: multilayer fabrication and characterization10.3390/jfb13020047 ↗
10. Advancements in gelatin-based hydrogel systems for biomedical applications: a state-of-the-art review10.1016/j.ijbiomac.2023.127143 ↗
11. A comparative study of Gelatin/HPMC/HA and Gel/HPMC/TCP Nanocomposites for bone tissue regeneration10.1007/s10924-023-02823-z ↗
12. Bioactive glasses for soft and hard tissue healing applications—a short review10.3390/app13106151 ↗
13. Borate Bioactive Glasses (BBG): bone regeneration, wound healing applications, and future directions10.1021/acsabm.2c00384 ↗
14. Bioactive glass 1393 promotes angiogenesis and accelerates wound healing through ROS/P53/MMP9 signaling pathway10.1016/j.reth.2024.05.016 ↗
15. Synthesis, structural characterization, and antibacterial studies of new borate 13–93B3 bioglasses with low copper dopant10.21608/ejchem.2022.84829.4137 ↗
16. Ion release, hydroxyapatite conversion, and cytotoxicity of boron‐containing bioactive glass scaffolds10.1111/ijag.12206 ↗
17. Bioactive borate glass scaffold for bone tissue engineering10.1016/j.jnoncrysol.2007.10.003 ↗
18. Cerium oxide nanoparticles: properties, biosynthesis and biomedical application10.1039/D0RA04736H ↗
19. Direct evidence for hydroxyl radical scavenging activity of cerium oxide nanoparticles10.1021/jp109819u ↗
20. Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications10.1016/j.mtbio.2021.100150 ↗
21. A brief review on cerium oxide (CeO2NPs)-based scaffolds: recent advances in wound healing applications10.3390/mi14040865 ↗
22. Sequential therapy for bone regeneration by cerium oxide-reinforced 3D-printed bioactive glass scaffolds10.1021/acsnano.2c09855 ↗
23. Synthesis and characterization of cerium- and gallium-containing borate bioactive glass scaffolds for bone tissue engineering10.1007/s10856-014-5368-0 ↗
24. In vivo evaluation of cerium, gallium and vanadium-doped borate-based bioactive glass scaffolds using rat subcutaneous implantation model10.1016/j.ceramint.2016.04.033 ↗
25. Cerium containing bioactive glasses: a review10.1021/acsbiomaterials.1c00414 ↗
26. How useful is SBF in predicting in vivo bone bioactivity?10.1016/j.biomaterials.2006.01.017 ↗
27. How do we determine the efficacy of an antibacterial surface? A review of standardised antibacterial material testing methods10.3390/antibiotics10091069 ↗
28. Influence of the replacement of silica by boron trioxide on the properties of bioactive glass scaffolds10.1111/ijag.15894 ↗
29. Physical properties of zinc, silver, or cerium ion doped borate glass incorporated PCL/gelatin electrospun fibers and their interaction with NG108-15 neural cells10.1007/s10856-025-06863-w ↗
30. In vitro assessment of the bioactivity of borate-based bioactive glasses: how different conditions influence hydroxyapatite formation10.1007/s00339-025-08876-2 ↗
31. Processing and characterization of aligned electrospun gelatin/polycaprolactone nanofiber mats incorporating borate glass (13–93B3) microparticles10.1088/1748-605X/acf0ad ↗
32. Strontium borate glass: potential biomaterial for bone regeneration10.1098/rsif.2009.0504 ↗
33. Synthesis and characterization of CeO2 nanoparticles via solution combustion method for photocatalytic and antibacterial activity studies10.1002/open.201402046 ↗
34. Catalytic dye oxidation over CeO2 nanoparticles supported on regenerated cellulose membrane10.9767/bcrec.17.3.15384.554-564 ↗
35. Role of pH on synthesis and characterization of cerium oxide (CeO2) nano particles by modified co-precipitation method10.1016/j.vacuum.2018.12.002 ↗
36. Synthesis of Cerium Oxide (CeO2) nanoparticles using simple CO-precipitation method
37. Use and mis-use of x-ray photoemission spectroscopy Ce3d spectra of Ce2O3 and CeO210.1088/1361-648X/aad248 ↗
38. Valence state manipulation of cerium oxide nanoparticles on a titanium surface for modulating cell fate and bone formation10.1002/advs.201700678 ↗
39. The effect of cerium valence states at cerium oxide nanoparticle surfaces on cell proliferation10.1016/j.biomaterials.2014.01.074 ↗
40. Study of nanoparticles aggregation/agglomeration in polymer particulate nanocomposites by mechanical properties10.1016/j.compositesa.2016.01.020 ↗
41. Preparation and characterization of gelatin-hydroxyapatite composite for bone tissue engineering
42. Improvement in hygroscopic property of macro-defect free cement modified with hypromellose/potassium methyl siliconate copolymer and pulverized fly ash10.1007/s10973-022-11447-9 ↗
43. Development and evaluation of a polyvinylalcohol—cellulose derivative-based film with povidone-iodine predicted for wound treatment10.3390/polym12061271 ↗
44. A novel injectable boron doped-mesoporous nano bioactive glass loaded-alginate composite hydrogel as a pulpotomy filling biomaterial for dentin regeneration10.1186/s12903-024-04808-3 ↗
45. Influence of CeO2 on the structure and properties of borosilicate glass: an investigation from the perspective besides the refining process10.1016/j.jnoncrysol.2023.122690 ↗
46. Experimental and in-silico computational modeling of cerium oxide nanoparticles functionalized by gelatin as an eco-friendly anti-corrosion barrier on X60 steel alloys in acidic environments10.3390/polym14132544 ↗
47. Preparation and characterization of gelatin/cerium(III) film10.1016/S1002-0721(09)60195-2 ↗
48. FT-IR spectroscopy for the identification of binding sites and measurements of the binding interactions of important metal ions with bovine serum albumin10.3390/scipharm87010005 ↗
49. Cerium(IV) chitosan-based hydrogel composite for efficient adsorptive removal of phosphates(V) from aqueous solutions10.1038/s41598-023-40064-1 ↗
50. XPS investigation on the reactivity of surface imine groups with TFAA10.1002/ppap.201800160 ↗
51. Nanoengineering of chitosan sponges via atomic layer deposition of ZnO for water remediation technologies10.1002/admi.202400831 ↗
52. Effect of melt-derived bioactive glass particles on the properties of chitosan scaffolds10.3390/jfb10030038 ↗
53. Bone grafts: which is the ideal biomaterial?,”10.1111/jcpe.13058 ↗
54. Optimizing scaffold pore size for tissue engineering: insights across various tissue types10.3389/fbioe.2024.1444986 ↗
55. 13–93 bioactive glass/alginate composite scaffolds 3D printed under mild conditions for bone regeneration10.1039/C6RA27669E ↗
56. Preparation and characterization of borate bioactive glass cross-linked PVA hydrogel10.1016/j.jnoncrysol.2017.07.017 ↗
57. Bone Regeneration: a Review of Current Treatment Strategies10.3390/jcm14061838 ↗
58. Production, structural characterization and in vitro biological evaluation of a new bioactive glass with the composition of SiO2-Na2O-CaO-P2O5-B2O3-MgO-V2O510.1016/j.inoche.2025.114326 ↗
59. Towards the surface hydroxyl species in CeO2 nanoparticles10.1039/C9NR06032D ↗
60. CeO2 nanoparticle-containing polymers for biomedical applications: a review10.3390/polym13060924 ↗
61. Chitosan/PVA hydrogels incorporated with green synthesized cerium oxide nanoparticles for wound healing applications10.1016/j.eurpolymj.2020.109853 ↗
62. Solubility of nanocrystalline cerium dioxide: experimental data and thermodynamic modeling10.1021/acs.jpcc.6b05650 ↗
63. In vitro evaluation of borate-based bioactive glass scaffolds prepared by a polymer foam replication method10.1016/j.msec.2009.05.013 ↗
64. Dissolution of borate and borosilicate bioactive glasses and the influence of ion (Zn, Cu) doping in different solutions10.1016/j.jnoncrysol.2018.08.037 ↗
65. Conversion of melt-derived microfibrous borate (13–93B3) and silicate (45S5) bioactive glass in a simulated body fluid10.1007/s10856-012-4831-z ↗
66. Conversion of borate-based glass scaffold to hydroxyapatite in a dilute phosphate solution10.1088/1748-6041/5/1/015005 ↗
67. Synthesis of cerium oxide nanoparticles using various methods: implications for biomedical applications10.3390/nano10020242 ↗
68. Cytocompatibility of potential bioactive cerium-doped glasses based on 45S510.3390/ma12040594 ↗
69. in vitro bioactive characteristics of borate-based glasses with controllable degradation behavior10.1111/j.1551-2916.2006.01358.x ↗
70. Fourier transform infrared spectroscopy investigation of water microenvironments in polyelectrolyte multilayers at varying temperatures10.1039/c9sm02478f ↗
71. Cerium doping of 45S5 bioactive glass improves redox potential and cellular bioactivity10.1038/s41598-024-66417-y ↗
72. Recent advance in surface modification for regulating cell adhesion and behaviors10.1515/ntrev-2020-0076 ↗
73. Synthesis and characterization of CeO2-incorporated mesoporous calcium-silicate materials10.1016/j.micromeso.2014.06.018 ↗
74. Cerium (III) and (IV) containing mesoporous glasses/alginate beads for bone regeneration: bioactivity, biocompatibility and reactive oxygen species activity10.1016/j.msec.2019.109971 ↗
75. Effects of engineered cerium oxide nanoparticles on bacterial growth and viability10.1128/AEM.00650-10 ↗
76. The use of cerium compounds as antimicrobials for biomedical applications10.3390/molecules27092678 ↗
77. Ion incorporation into bone grafting materials10.1111/prd.12533 ↗