Synthesis of poly(2-isopropyl-2-oxazoline)-b-poly(2-phenyl-2-oxazoline)-b-poly(2-isopropyl-2-oxazoline) and its self-assembly into polymersomes: Temperature-dependent aqueous solution behavior
Source abstract
Abstract
This study reports on synthesis of poly(2-isopropyl-2-oxazoline)-b-poly(2-phenyl-2-oxazoline)-b-poly(2-isopropyl-2-oxazoline) (PiPOX-b-PPhOX-b-PiPOX) by a three-step sequential monomer addition through cationic ring opening polymerization (CROP). Self-assembly of PiPOX-b-PPhOX-b-PiPOX was triggered through solvent shifting or double emulsion methods, resulting in formation of polymersomes with hydrophilic and temperatureresponsive PiPOX inner/outer coronae and hydrophobic PPhOX membrane. Double emulsion method provided smaller polymersomes with greater colloidal stability. Increasing the temperature of the polymersome solution led to formation of polymersome clusters and this transition was found to be irreversible. Glucose oxidase (GOx) encapsulated polymersomes were used as nanoreactors for GOx/horseradish peroxidase (HRP) cascade reactions. The increase in the rate of the enzymatic reaction with increasing temperature was found to be smaller for the encapsulated enzymes compared to free enzymes.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced Polymer Synthesis and Characterization · biodegradable polymer synthesis and properties · Molecular Sensors and Ion Detection
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (76)
Show source-provided references
1. Poly(2-oxazoline)s: a polymer class with numerous potential applications10.1002/anie.200901607 ↗
2. Biocompatibility and immunocompatibility assessment of poly(2-oxazolines)
3. Conjugation of ciprofloxacin with poly(2-oxazoline)s and polyethylene glycol via end groups10.1021/acs.bioconjchem.5b00393 ↗
4. Antibiofouling properties of plasma-deposited oxazoline-based thin films10.1021/acsami.6b00330 ↗
5. Poly(2-oxazoline)s as materials for biomedical applications10.1007/s10856-013-5034-y ↗
6. Polyoxazoline: chemistry, properties, and applications in drug delivery10.1021/bc200049d ↗
7. Synthesis of pH- and thermoresponsive poly(2- n -propyl-2-oxazoline) based copolymers10.1002/pola.28011 ↗
8. Tuning the LCST of poly(2-oxazoline)s by varying composition and molecular weight: alternatives to poly(N-isopropylacrylamide)?10.1039/b813140f ↗
9. Alternative to poly(2–isopropyl‐2–oxazoline) with a reduced ability to crystallize and physiological LCST10.3390/ijms22042221 ↗
10. Poly(2-oxazoline)-containing triblock copolymers: synthesis and applications10.1080/15583724.2018.1496930 ↗
11. Polyoxazoline: a review article from polymerization to smart behaviors and biomedical applications10.1016/j.eurpolymj.2022.111484 ↗
12. Aggregation behavior and interaction with human serum albumin of 2-oxazoline block copolymers in aqueous solutions10.1002/macp.1997.021980109 ↗
13. Organization of poly(2-ethyl-2-oxazoline)-block-poly(2-phenyl-2-oxazoline) copolymers in water solution10.1016/j.polymer.2010.03.043 ↗
14. Solubility behavior of amphiphilic block and random copolymers based on 2-ethyl-2-oxazoline and 2-nonyl-2-oxazoline in binary water-ethanol mixtures10.1002/pola.23168 ↗
15. Microwave-assisted cationic ring-opening polymerization of 2-oxazolines: a powerful method for the synthesis of amphiphilic triblock copolymers10.1021/ma060952a ↗
16. Multiple micellar morphologies from tri- and tetrablock copoly(2-oxazoline)s in binary water-ethanol mixtures10.1002/pola.24089 ↗
17. Well-defined amphiphilic poly(2-oxazoline) ABA-triblock copolymers and their aggregation behavior in aqueous solution10.1002/marc.201200192 ↗
18. Temperature- and pH-dependent aggregation behavior of hydrophilic dual-sensitive poly(2-oxazoline)s block copolymers as latent amphiphilic macromolecules10.1016/j.eurpolymj.2016.11.014 ↗
19. Unravelling the excellent chemical stability and bioavailability of solvent responsive curcumin-loaded 2-ethyl-2-oxazoline-grad-2-(4-dodecyloxyphenyl)-2-oxazoline copolymer nanoparticles for drug delivery10.1021/acs.biomac.8b00057 ↗
20. Alkyl chain length in poly(2-oxazoline)-based amphiphilic gradient copolymers regulates the delivery of hydrophobic molecules: a case of the biodistribution and the photodynamic activity of the photosensitizer hypericin10.1021/acs.biomac.1c00768 ↗
21. Amphiphilic gradient copolymers of 2-methyl- and 2-phenyl-2-oxazoline: self-organization in aqueous media and drug encapsulation10.1002/pola.25888 ↗
22. Solvent-control over monomer distribution in the copolymerization of 2-oxazolines and the effect of a gradient structure on self-assembly10.1039/C9PY00927B ↗
23. Solution behavior of hydrolyzed gradient methyl/phenyl oxazoline copolymers and complexation with DNA10.1002/macp.201400552 ↗
24. Lamellarity and size distributions in mixed DPPC/amphiphilic poly(2-oxazoline) gradient copolymer vesicles and their temperature response10.1016/j.chemphyslip.2020.105008 ↗
25. DPPC/poly(2-methyl-2-oxazoline)-grad-poly(2-phenyl-2-oxazoline) chimeric nanostructures as potential drug nanocarriers10.1007/s11051-013-1685-3 ↗
26. DPPC:MPOx chimeric advanced drug delivery nano Systems (chi-aDDnSs): physicochemical and structural characterization, stability and drug release studies10.1016/j.ijpharm.2013.03.052 ↗
27. One-Pot synthesis of an amphiphilic ABC triblock copolymer PEO-b-PEHOx-b-PEtOz and its self-assembly into nanoscopic asymmetric polymersomes10.1021/acs.macromol.0c02301 ↗
28. Probing bioinspired transport of nanoparticles into polymersomes10.1002/anie.201108421 ↗
29. Vesicles with asymmetric membranes from amphiphilic ABC triblock copolymers10.1039/b209352a ↗
30. Asymmetric ABC-triblock copolymer membranes induce a directed insertion of membrane proteins10.1002/mabi.200400065 ↗
31. Polymerized ABA triblock copolymer vesicles10.1021/la990951u ↗
32. Reconstitution of channel proteins in (Polymerized) ABA triblock copolymer membranes10.1002/1521-3773(20001215)39:24<4599::AID-ANIE4599>3.0.CO;2-Y ↗
33. A nanocompartment system (Synthosome) designed for biotechnological applications10.1016/j.jbiotec.2005.10.025 ↗
34. Nanoreactors based on (polymerized) ABA-triblock copolymer vesicles10.1039/b004280n ↗
35. Therapeutic nanoreactors: combining chemistry and biology in a novel triblock copolymer drug delivery system10.1021/nl051523d ↗
36. Unexpected reactivity switch in the statistical copolymerization of 2-oxazolines and 2-oxazines enabling the one-step synthesis of amphiphilic gradient copolymers10.1021/jacs.9b02607 ↗
37. Self-Assembly, drug encapsulation, and cellular uptake of block and gradient copolymers of 2-methyl-2-oxazine and 2-n-propyl/butyl-2-oxazoline10.1021/acs.macromol.1c01794 ↗
38. Macromolecular design and preparation of polymersomes10.1039/D0PY01247E ↗
39. Polymersomes for drug delivery and other biomedical applications
40. Positional assembly of enzymes in polymersome nanoreactors for cascade reactions10.1002/anie.200701125 ↗
41. Artificial organelles: towards adding or restoring intracellular activity10.1002/cbic.202000850 ↗
42. Temperature-responsive polymersomes of poly(3-methyl-N-vinylcaprolactam)-block-poly(N-vinylpyrrolidone) to decrease doxorubicin-induced cardiotoxicity10.1021/acs.biomac.9b01026 ↗
43. Thermally responsive vesicles and their structural “locking” through polyelectrolyte complex formation10.1002/anie.200602168 ↗
44. Temperature-controlled assembly and release from polymer vesicles of poly(ethylene oxide)-block-poly(N-isopropylacrylamide)10.1002/adma.200601019 ↗
45. Temperature-sensitive polymersomes for controlled delivery of anticancer drugs10.1021/acs.chemmater.5b03048 ↗
46. Dually responsive poly(N-vinylcaprolactam)-b-poly(dimethylsiloxane)-b-poly(N-vinylcaprolactam) polymersomes for controlled delivery10.3390/molecules27113485 ↗
47. Effect of temperature and hydrophilic ratio on the structure of poly(N-vinylcaprolactam)-block-poly(dimethylsiloxane)-block-poly(N-vinylcaprolactam) polymersomes10.1021/acsapm.8b00259 ↗
48. Modulation of the lower critical solution temperature of 2-alkyl-2-oxazoline copolymers10.1007/s00396-007-1781-y ↗
49. Enhanced anti-tumor efficacy by co-delivery of doxorubicin and paclitaxel with amphiphilic methoxy PEG-PLGA copolymer nanoparticles10.1016/j.biomaterials.2011.07.032 ↗
50. Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies10.1006/abio.1996.0171 ↗
51. Linearization of the Bradford protein assay
52. Precise control of lower critical solution temperature of thermosensitive poly(2-isopropyl-2-oxazoline) via gradient copolymerization with 2-ethyl-2-oxazoline as a hydrophilic comonomer10.1021/ma0605548 ↗
53. The role of chain molecular weight and Hofmeister series ions in thermal aggregation of poly(2-isopropyl-2-oxazoline) grafted nanoparticles10.3390/polym10040451 ↗
54. Automated parallel temperature optimization and determination of activation energy for the living cationic polymerization of 2-ethyl-2-oxazoline10.1002/marc.200390017 ↗
55. C. Legros. Engineering of Poly(2-oxazoline)s for a Potential Use in Biomedical Application, Université de Bordeaux, 2014.
56. One-Pot synthesis of charged amphiphilic diblock and triblock copolymers via high-throughput Cu(0)-mediated polymerization10.3390/polym9080320 ↗
57. S.R. Barnes, J.S. Riffle, J.E. Mcgrath, R.M. Davis, Size Exclusion Chromatography of Poly(2-ethyl-2-oxazoline) Homopolymers and Poly(ethylene oxide)-b-Poly(2-ethyl-2-oxazoline) Copolymers, Virginia Polytechnic Institute and State University, 2013.
58. Microphase segregation and selective chain scission of poly(2–methyl‐2–oxazoline)‐ block ‐polystyrene10.1002/pola.29396 ↗
59. Sulfolane as common rate accelerating solvent for the cationic ring-opening polymerization of 2-oxazolines10.1021/acsmacrolett.5b00392 ↗
60. Ligand-directed active tumor-targeting polymeric nanoparticles for cancer chemotherapy10.1021/bm5003009 ↗
61. Engineering polymersomes for diagnostics and therapy10.1002/adhm.201701276 ↗
62. Density functional simulation of spontaneous formation of vesicle in block copolymer solutions10.1063/1.2463426 ↗
63. Techniques to control polymersome size10.1021/acs.macromol.5b01500 ↗
64. Synthesis and complex self-assembly of amphiphilic block copolymers with a branched hydrophobic poly(2-oxazoline) into multicompartment micelles, pseudo-vesicles and yolk/shell nanoparticles10.1039/C9PY01559K ↗
65. Multicompartment polymer vesicles with artificial organelles for signal-triggered cascade reactions including cytoskeleton formation10.1002/adfm.202002949 ↗
66. Mimicking cellular signaling pathways within synthetic multicompartment vesicles with triggered enzyme activity and induced ion channel recruitment10.1002/adfm.201904267 ↗
67. Multicompartment polymersomes from double emulsions10.1002/anie.201006023 ↗
68. Fabrication of polymersomes using double-emulsion templates in glass-coated stamped microfluidic devices10.1002/smll.201000798 ↗
69. Generation of polymerosomes from double-emulsions10.1021/la050797d ↗
70. The effect of stabilizer on the mechanical response of double-emulsion-templated polymersomes10.1002/marc.201400472 ↗
71. Poly(2-oxazoline)s as smart bioinspired polymers10.1002/marc.200900683 ↗
72. Polymeric nanoparticle engineering: from temperature-responsive polymer mesoglobules to gene delivery systems10.1021/bm501194g ↗
73. Formation of polyamide nanofibers by directional crystallization in aqueous solution10.1002/anie.200703486 ↗
74. Self-assemblies of thermoresponsive poly(N-vinylcaprolactam) polymers for applications in biomedical field10.1021/acsapm.9b00863 ↗
75. Distinct morphological transitions of photoreactive and thermoresponsive vesicles for controlled release and nanoreactors10.1021/acs.macromol.6b01374 ↗
76. Glucose oxidase, an enzyme “ferrari”: its structure, function, production and properties in the light of various industrial and biotechnological applications10.3390/biom12030472 ↗