DataVXBiomaterials ResearchPaper
journal article·2025-01Open access

Engineered Endometrial Clear Cell Cancer-on-a-Chip Reveals Early Invasion-Metastasis Cascade of Cancer Cells

Chengpan Li, Jing Pan, Zhengdi Shi, Xinyan Zeng, Xiaoping Xia, Xiaogang He, Wei Wang, Bensheng Qiu, Weiping Ding, Dabing Huang

Biomaterials Research· Volume 29
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Source abstract

Abstract

Endometrial clear cell cancer (ECCC) is an extremely rare and highly malignant subtype of endometrial cancer. For most ECCC patients, cancer metastasis is the major cause of death. To date, due to the complexity of cancer evolution and the small number of cases, the metastasis of ECCC at the early stage remains largely unknown. Herein, we modeled the early invasion-metastasis cascade of ECCC by coculturing the ECCC patient-derived tumor cells (PDTCs) and primary human vascular endothelial cells on a microfluidic chip. With the chip, we for the first time replicated the dynamic migration of PDTCs into the surrounding stroma, including the intravasation and extravasation of PDTCs through the capillaries/microvessels, and presented the changes in the morphology and permeability of capillaries, with the decreased diameter and the increased permeability after cancer metastasis. We found that PDTCs were more invasive than the common endometrial adenocarcinoma cells. In addition, we preliminarily explored the inhibition of drugs on the early PDTC infiltration. This study provides new ideas for better understanding of ECCC evolution.
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Research context

OpenAlex topics: Advanced Biosensing Techniques and Applications · 3D Printing in Biomedical Research · Cancer Cells and Metastasis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Electronic Engineering and Information Science, School of Information Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.

Department of Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China.

Department of Integrated Traditional Chinese and Western Medicine, Anhui Medical University, Hefei, Anhui 230032, China.

Department of Obstetrics and Gynecology, Anhui Provincial Children’s Hospital, Children’s Hospital of Fudan University Anhui Hospital, Children’s Hospital of Anhui Medical University

Department of Urology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China.

Funding

Fundamental Research Funds for the Central Universities · Key Research and Development Program of Anhui Province · Anhui Provincial Clinical Medical Research Translation Special Program · National Natural Science Foundation of China · Anhui Provincial Science and Technology Major Project

References (48)

Show source-provided references
1. 10.1016/S0140-6736(22)00323-310.1016/S0140-6736(22)00323-3 ↗
2. 10.3389/fonc.2022.96115510.3389/fonc.2022.961155 ↗
3. 10.1002/path.494710.1002/path.4947 ↗
4. 10.3322/caac.2156110.3322/caac.21561 ↗
5. 10.1016/j.ygyno.2022.01.01210.1016/j.ygyno.2022.01.012 ↗
6. 10.1126/science.120354310.1126/science.1203543 ↗
7. 10.1111/his.1258110.1111/his.12581 ↗
8. 10.1186/s12885-020-07567-w10.1186/s12885-020-07567-w ↗
9. 10.1038/s41568-021-00427-010.1038/s41568-021-00427-0 ↗
10. 10.1007/978-3-030-34025-4_2010.1007/978-3-030-34025-4_20 ↗
11. 10.1016/j.ijsu.2019.10.01510.1016/j.ijsu.2019.10.015 ↗
12. 10.1016/j.trecan.2020.10.00910.1016/j.trecan.2020.10.009 ↗
13. 10.1016/j.bios.2022.11462610.1016/j.bios.2022.114626 ↗
14. 10.1016/j.mtbio.2023.10072410.1016/j.mtbio.2023.100724 ↗
15. 10.1039/c3lc41320a10.1039/c3lc41320a ↗
16. 10.1039/C5LC01050K10.1039/C5LC01050K ↗
17. 10.1038/s41551-020-00621-910.1038/s41551-020-00621-9 ↗
18. 10.1063/5.001121610.1063/5.0011216 ↗
19. 10.1007/s10439-017-1797-510.1007/s10439-017-1797-5 ↗
20. 10.1093/humrep/deab18610.1093/humrep/deab186 ↗
21. 10.1038/s41574-022-00725-z10.1038/s41574-022-00725-z ↗
22. 10.1016/j.jbiomech.2015.11.03110.1016/j.jbiomech.2015.11.031 ↗
23. 10.1021/acsbiomaterials.2c0034210.1021/acsbiomaterials.2c00342 ↗
24. 10.1007/s13577-019-00291-z10.1007/s13577-019-00291-z ↗
25. 10.1038/s43856-021-00019-x10.1038/s43856-021-00019-x ↗
26. Izuishi K, Kato K, Ogura T, Kinoshita T, Esumi H. Remarkable tolerance of tumor cells to nutrient deprivation: Possible new biochemical target for cancer therapy. Cancer Res. 2000;60(21):6201–6207.
27. 10.1158/0008-5472.CAN-07-146210.1158/0008-5472.CAN-07-1462 ↗
28. 10.1038/nprot.2008.17210.1038/nprot.2008.172 ↗
29. 10.1016/0166-2236(89)90040-410.1016/0166-2236(89)90040-4 ↗
30. Du X, Zou R, Du K, Huang D, Miao C, Qiu B, Ding W, Li D. Modeling colorectal cancer-induced liver portal vein microthrombus on a hepatic lobule chip. ACS Appl Mater Interfaces. 2023;15:56859–56868.
31. 10.1039/C5LC01028D10.1039/C5LC01028D ↗
32. 10.1088/1758-5090/acc35d10.1088/1758-5090/acc35d ↗
33. 10.1002/adhm.20170125710.1002/adhm.201701257 ↗
34. 10.2147/DDDT.S9723510.2147/DDDT.S97235 ↗
35. 10.1039/D2LC00082B10.1039/D2LC00082B ↗
36. 10.1002/cam4.166410.1002/cam4.1664 ↗
37. 10.7150/jca.2537010.7150/jca.25370 ↗
38. 10.1016/S0002-9378(16)32994-510.1016/S0002-9378(16)32994-5 ↗
39. 10.1097/PAS.000000000000008510.1097/PAS.0000000000000085 ↗
40. 10.1111/his.1228610.1111/his.12286 ↗
41. Igbinigie E, Guo F, Jiang S-W, Kelley C, Li J. Dkk1 involvement and its potential as a biomarker in pancreatic ductal adenocarcinoma. J Comput Biol. 2019;488:226–234.
42. 10.1002/adma.20200594410.1002/adma.202005944 ↗
43. 10.1038/s41551-022-00853-x10.1038/s41551-022-00853-x ↗
44. 10.1158/0008-5472.CAN-19-156410.1158/0008-5472.CAN-19-1564 ↗
45. 10.1111/cas.1333610.1111/cas.13336 ↗
46. 10.21037/atm-22-646310.21037/atm-22-6463 ↗
47. 10.1039/D0LC00799D10.1039/D0LC00799D ↗
48. 10.1016/j.cub.2020.06.08110.1016/j.cub.2020.06.081 ↗