Cerebrovascular-Specific Extracellular Matrix Bioink Promotes Blood–Brain Barrier Properties
Source abstract
Abstract
Chronic neuroinflammation is a principal cause of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. The blood–brain barrier predominantly comprises endothelial cells, and their intercellular communication with pericytes and other cell types regulates neuroinflammation. Here, we develop a tubular, perfusable model of human cerebrovascular tissues to study neurodegenerative diseases using cerebrovascular-specific extracellular matrix bioink, derived from a complementary blend of brain- and blood-vessel-derived extracellular matrices. The endothelial cells and pericytes in the bioprinted constructs spontaneously self-assemble into a dual-layered structure, closely mimicking the anatomy of the blood–brain barrier. Moreover, the mature cerebrovascular tissue shows physiological barrier functions and neuroinflammatory responses, indicating its potential for developing models of neuroinflammation-related pathologies. Collectively, our study demonstrates that furnishing a cerebrovascular-specific microenvironment can guide the cells to have native-like anatomical relevance and functional recapitulation in vitro.
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Research context
OpenAlex topics: 3D Printing in Biomedical Research · Barrier Structure and Function Studies · Tissue Engineering and Regenerative Medicine
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Division of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37666, Republic of Korea.
Department of Convergence IT Engineering, POSTECH, Pohang 37666, Republic of Korea.
School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.
Department of Convergence Biosystems Engineering, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Department of Neurosurgery, Cancer Research Institute, Hypoxia Ischemia Disease Institute, Seoul National University, Seoul 03080, Republic of Korea.
Advanced Institutes of Convergence Technology, Seoul National University, Suwon-si, Republic of Korea.
Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
Institute of Convergence Science, Yonsei University, Seoul 03722, Republic of Korea.
Funding
Ministry of Education · Ministry of Health and Welfare
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