Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles
Source abstract
Abstract
Coated calcium phosphate nanoparticles were prepared for cell transfection. A calcium phosphate nanoparticle served as core which was then coated with DNA for colloidal stabilisation. The efficiency of transfection could be considerably increased by adding another layer of calcium phosphate on the surface, thereby incorporating DNA into the particle and preventing its degradation within the cell by lysosomes. A subsequent outermost layer of DNA on the calcium phosphate gave a colloidal stabilisation. The efficiency of such multi-shell particles was significantly higher than that of simple DNA-coated calcium phosphate nanoparticles. The transfection efficiency of EGFP-encoding DNA was tested with different cell lines (T-HUVEC, HeLa, and LTK). The dispersions were stable and could be used for transfection after 2 weeks of storage at 4 degrees C without loss of efficiency.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · DNA and Nucleic Acid Chemistry
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (44)
Show source-provided references
1. Genetic exchange in Salmonella10.1128/jb.64.5.679-699.1952 ↗
2. Transfection with baculovirus DNA10.1016/0042-6822(80)90505-X ↗
3. Transfer of genes to humans: early lessons and obstacles to success10.1126/science.270.5235.404 ↗
4. Immune responses to transgene-encoded proteins limit the stability of gene expression after injection of replication-defective adenovirus vectors10.1038/nm0596-545 ↗
5. Optimization of electroporation for transfection of mammalian cell lines10.1016/0003-2697(89)90429-6 ↗
6. Electroporation-mediated gene transfer efficiency is reduced by linear plasmid carrier DNAs10.1016/0003-2697(92)90429-B ↗
7. High efficiency transformation by direct microinjection of DNA into cultured mammalian cells10.1016/0092-8674(80)90358-X ↗
8. In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment10.1073/pnas.87.24.9568 ↗
9. Infectious poliovirus RNA: a sensitive method of assay10.1016/0042-6822(65)90126-1 ↗
10. Semi-automated synthesis and screening of a large library of degradable cationic polymers for gene delivery10.1002/ange.200351244 ↗
11. Characterization of liposome-mediated gene delivery: expression, stability, and pharmacokinetics of plasmid DNA10.1038/sj.gt.3300350 ↗
12. Calcium enhances the transfection potency of stabilized plasmid-lipid particles10.1016/j.ab.2005.02.033 ↗
13. A new technique for the assay of infectivity of human adenovirus 5 DNA10.1016/0042-6822(73)90341-3 ↗
14. Silica nanoparticles modified with aminosilanes as carriers for plasmid DNA10.1016/S0378-5173(99)00435-4 ↗
15. DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations10.1073/pnas.90.24.11478 ↗
16. Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake10.1016/S0142-9612(01)00267-8 ↗
17. Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells
18. DNA encapsulated magnesium and manganous phosphate nanoparticles: potential non-viral vectors for gene delivery10.1016/j.biomaterials.2004.06.039 ↗
19. The uptake and stability of simian-virus-40 DNA after calcium phosphate transfection of CV-1 cells10.1042/bj2180475 ↗
20. Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation10.1093/nar/24.4.596 ↗
21. Calcium phosphate as a gene carrier: electron microscopy10.1016/S0142-9612(96)00120-2 ↗
22. Fast convenient, and effective method to transiently transfect primary hippocampal neurons10.1002/(SICI)1097-4547(19991215)58:6<831::AID-JNR10>3.0.CO;2-M ↗
23. DNA/calcium phosphate precipitates mixed with medium are stable and maintain high transfection efficiency10.1006/abio.1999.4429 ↗
24. Size-dependency of nanoparticle-mediated gene transfection: studies with fractionated nanoparticles10.1016/S0378-5173(02)00315-0 ↗
25. Techniques for gene transfer into neurons10.1016/S0959-4388(02)00365-3 ↗
26. Calcium phosphate nanoparticles as novel non-viral vectors for targeted gene delivery10.1016/S0378-5173(02)00452-0 ↗
27. Transfection of adherent and suspended cells by calcium phosphate10.1016/j.ymeth.2003.11.011 ↗
28. Size-controlled formation of a calcium phosphate-based organic–inorganic hybrid vector for gene delivery using poly(ethylene glycol)-block-poly(aspartic acid)10.1002/adma.200305782 ↗
29. Liposomes in gene therapy10.1016/0169-409X(96)00002-6 ↗
30. Cationic liposomes are a strong adjuvant for a DNA vaccine of human immunodeficiency virus type 110.1089/aid.1997.13.1421 ↗
31. In vitro gene delivery by degraded polyamidoamine dendrimers10.1021/bc9600630 ↗
32. High-efficiency transformation of mammalian cells by plasmid DNA
33. A critical parameter determining the aging of DNA-calcium-phosphate precipitates10.1006/abio.1996.9948 ↗
34. High-efficiency gene delivery for expression in mammalian cells by nanoprecipitates of Ca–Mg phosphate10.1016/j.gene.2004.07.015 ↗
35. Block copolymer self-assembly into monodispersive nanoparticles with hybrid core of antisense DNA and calcium phosphate10.1021/la011736s ↗
36. Transfection of cells with custom-made calcium phosphate nanoparticles coated with DNA10.1039/b401644k ↗
37. Mechanisms of DNA entry into mammalian cells. II. Phagocytosis of calcium phosphate DNA co-precipitate visualized by electron microscopy10.1016/0014-4827(82)90336-6 ↗
38. Intracellular routing of plasmid DNA during non-viral gene transfer10.1016/j.addr.2004.12.008 ↗
39. Spontaneous transformation and immortalization of human endothelial cells10.1007/BF02624456 ↗
40. Optimization of calcium phosphate transfection for bovine chromaffin cells: relationship to calcium phosphate precipitate formation10.1006/abio.1995.1216 ↗
41. Liposome-directed growth of calcium phosphate nanoshells10.1002/1521-4095(20020404)14:7<532::AID-ADMA532>3.0.CO;2-4 ↗
42. High transfection efficiency of human umbilical vein endothelial cells using an optimized calcium phosphate method10.1006/abio.2001.5200 ↗
43. Temporal targeting of tumour cells and neovasculature with a nanoscale delivery system10.1038/nature03794 ↗
44. Characterization of aqueous dispersions of Fe3O4 nanoparticles and their biomedical applications10.1016/j.biomaterials.2004.03.016 ↗