Cell directional migration and oriented division on three-dimensional laser-induced periodic surface structures on polystyrene
Source abstract
Abstract
The extracellular matrix in animal tissues usually provides a three-dimensional structural support to cells in addition to performing various other important functions. In the present study, wavy submicrometer laser-irradiated periodic surface structures (LIPSS) were produced on a smooth polystyrene film by polarized laser irradiation with a wavelength of 266 nm. Rat C6 glioma cells exhibited directional migration and oriented division on laser-irradiated polystyrene, which was parallel to the direction of LIPSS. However, rat C6 glioma cells on smooth polystyrene moved in a three-step invasion cycle, with faster migration speed than that on laser-irradiated polystyrene. In addition, focal adhesions examined by immunostaining focal adhesion kinase in human epithelial carcinoma HeLa cells were punctuated on smooth polystyrene, whereas dash-like on laser-irradiated polystyrene. We hypothesized that LIPSS on laser-irradiated polystyrene acted as an anisotropic and persistent mechanical stimulus to guide cell anisotropic spreading, migration and division through focal adhesions.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Cellular Mechanics and Interactions · Collagen: Extraction and Characterization · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (44)
Show source-provided references
1. Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions10.1038/ncb1536 ↗
2. Regulation of cell polarity during eukaryotic chemotaxis: the chemotactic compass10.1016/S0955-0674(02)00310-1 ↗
3. Cofilin promotes actin polymerization and defines the direction of cell motility10.1126/science.1094561 ↗
4. A Rac switch regulates random versus directionally persistent cell migration10.1083/jcb.200503152 ↗
5. Cell shape and cell division10.1016/j.ceb.2006.10.001 ↗
6. The extracellular matrix guides the orientation of the cell division axis10.1038/ncb1307 ↗
7. Experimental and theoretical study of mitotic spindle orientation10.1038/nature05786 ↗
8. Cell movement is guided by the rigidity of the substrate10.1016/S0006-3495(00)76279-5 ↗
9. Biomaterial surface-dependent neutrophil mobility10.1002/jbm.a.30015 ↗
10. Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients10.1016/j.biomaterials.2007.01.019 ↗
11. Geometric control of cell life and death10.1126/science.276.5317.1425 ↗
12. Nanopattern-induced changes in morphology and motility of smooth muscle cells10.1016/j.biomaterials.2005.01.058 ↗
13. Polymeric biomaterials for tissue and organ regeneration10.1016/S0927-796X(01)00035-3 ↗
14. Hydrophilic group formation and cell culturing on polystyrene Petri-dish modified by ion-assisted reaction10.1016/S0168-583X(00)00650-9 ↗
15. Plasma-treated polystyrene surfaces: model surfaces for studying cell-biomaterial interactions10.1016/j.biomaterials.2003.08.071 ↗
16. Fabrication of novel biomaterials through molecular self-assembly10.1038/nbt874 ↗
17. Surface-modification of polystyrene-microtitre plates via grafting of glycidylmethacrylate and coating of poly-glycidylmethacrylate10.1016/S0142-9612(99)00098-8 ↗
18. Surface modification of polymers by grafting
19. The effects of continuous and discontinuous groove edges on cell shape and alignment10.1016/S0014-4827(03)00159-9 ↗
20. Epithelial contact guidance on well-defined micro- and nanostructured substrates10.1242/jcs.00383 ↗
21. Cell interaction with three-dimensional sharp-tip nanotopography10.1016/j.biomaterials.2006.11.031 ↗
22. Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir–Blodgett lithography10.1016/j.biomaterials.2004.02.068 ↗
23. Oriented astroglial cell growth on micropatterned polystyrene substrates10.1016/j.biomaterials.2003.11.045 ↗
24. In vitro reaction of endothelial cells to polymer demixed nanotopography10.1016/S0142-9612(01)00424-0 ↗
25. Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications10.1016/j.biomaterials.2005.11.037 ↗
26. Characterisation of electrospun polystyrene scaffolds for three-dimensional in vitro biological studies10.1016/j.biomaterials.2006.01.026 ↗
27. Electrospinning of collagen nanofibers10.1021/bm015533u ↗
28. Influence of physicochemical properties of laser-modified polystyrene on bovine serum albumin adsorption and rat C6 glioma cell behavior10.1002/jbm.a.30764 ↗
29. Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion10.1007/s00441-002-0651-7 ↗
30. Laser-induced sub-half-micrometer periodic structure on polymer surfaces10.1063/1.111104 ↗
31. Laser generated microstructures10.1007/BF00617497 ↗
32. The centrosome in vertebrates: more than microtubule-organizing center10.1016/S0962-8924(01)02085-2 ↗
33. Chromosome segregation and aneuploidy series: centrosome control of the cell10.1016/j.tcb.2005.04.008 ↗
34. Focal adhesion kinase: in command and control of cell motility10.1038/nrm1549 ↗
35. Cell culturing on nanogrooved polystyrene petri dish induced by ultraviolet laser irradiation10.1016/S0167-577X(03)00408-7 ↗
36. Cell migration at a glance10.1242/jcs.02662 ↗
37. The effect of topographic characteristics on cell migration velocity10.1016/j.biomaterials.2006.06.002 ↗
38. Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction10.1083/jcb.146.5.1097 ↗
39. Glioma cell motility is associated with reduced transcription of proapoptotic and proliferation genes: a cDNA microarray analysis10.1023/A:1012253317934 ↗
40. Tensegrity I. Cell structure and hierarchical systems biology10.1242/jcs.00359 ↗
41. Tensegrity II. How structural networks influence cellular information processing networks10.1242/jcs.00360 ↗
42. Cell spreading and lamellipodial extension rate is regulated by membrane tension10.1083/jcb.148.1.127 ↗
43. Dynamics of C6 astrocytoma invasion into three-dimensional collagen gels10.1023/A:1012236830230 ↗
44. Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography10.1016/S0014-4827(02)00053-8 ↗