DataVXBiointerphasesPaper
journal article·2016-06-01

Subpopulations in purified platelets adhering on glass

Alessia Donati, Swati Gupta, Ilya Reviakine

Biointerphases· Volume 11· Issue 2
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Source abstract

Abstract

Understanding how platelet activation is regulated is important in the context of cardiovascular disorders and their management with antiplatelet therapy. Recent evidence points to different platelet subpopulations performing different functions. In particular, procoagulant and aggregating subpopulations have been reported in the literature in platelets treated with the GPVI agonists. How the formation of platelet subpopulations upon activation is regulated remains unclear. Here, it is shown that procoagulant and aggregating platelet subpopulations arise spontaneously upon adhesion of purified platelets on clean glass surfaces. Calcium ionophore treatment of the adhering platelets resulted in one platelet population expressing both the procoagulant and the adherent population markers phosphatidylserine and the activated form of GPIIb/IIIa, while all of the platelets expressed CD62P independently of the ionophore treatment. Therefore, all platelets have the capacity to express all three activation markers. It is concluded that platelet subpopulations observed in various studies reflect the dynamics of the platelet activation process.
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Research context

Ceramics & glasses

OpenAlex topics: Platelet Disorders and Treatments · Antiplatelet Therapy and Cardiovascular Diseases · Heparin-Induced Thrombocytopenia and Thrombosis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Karlsruhe Institute of Technology (KIT) Institute of Functional Interfaces (IFG), , Hermann-von Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany

CIC biomaGUNE Biosurfaces, , Paseo Miramón 182, San Sebastián 20009, Spain and Department of Biochemistry and Molecular Biology, , 28940 Leioa, Spain

University of the Basque Country Biosurfaces, , Paseo Miramón 182, San Sebastián 20009, Spain and Department of Biochemistry and Molecular Biology, , 28940 Leioa, Spain

Karlsruhe Institute of Technology (KIT) Institute of Functional Interfaces (IFG), , Hermann-von Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and Department of Bioengineering, , Seattle, Washington 98105

University of Washington Institute of Functional Interfaces (IFG), , Hermann-von Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and Department of Bioengineering, , Seattle, Washington 98105

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