Length computation of irradiated plasmid DNA molecules
Source abstract
Abstract
Compromised detection of short DNA fragments can result in underestimation of radiation-induced clustered DNA damage. The fragments can be detected with atomic force microscopy (AFM), followed by image analysis to compute the length of plasmid molecules. Plasmid molecules imaged with AFM are represented by open or closed curves, possibly with crossings. For the analysis of such objects, a dedicated algorithm was developed, and its usability was demonstrated on the AFM images of plasmid pBR322 irradiated with 60Co gamma rays. The analysis of the set of the acquired AFM images revealed the presence of DNA fragments with lengths shorter than 300 base pairs that would have been neglected by a conventional detection method.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Force Microscopy Techniques and Applications · Advanced Electron Microscopy Techniques and Applications · Integrated Circuits and Semiconductor Failure Analysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Radiation Dosimetry, Nuclear Physics Institute of the CAS 1 , Na Truhlarce 39/64, Praha 180 86, Czech Republic
Atominstitut, Technische Universität Wien 2 , Stadionallee 2, Wien 1020, Austria
MedAustron 3 , Marie Curie-Strasse 5, 2700 Wiener Neustadt, Austria
Department of Thin Films and Nanostructures, Institute of Physics of the CAS 4 , Cukrovarnicka 10/112, Praha 162 00, Czech Republic
Funding
European Space Agency · Grantová Agentura České Republiky
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