In vitro biomineralization on poly(vinyl alcohol)/biphasic calcium phosphate hydrogels

Biomineralized tissue is considered the final product of successful cell culture in bone tissue engineering. Dulbecco’s modified Eagle’s medium (DMEM) with fetal bovine serum (FBS) not only is used as a common culture medium but also provides a natural biomineralization environment, due to having similar ionic concentrations as blood plasma. Here, poly(vinyl alcohol) hydrogel with incorporated biphasic calcium phosphate nanoparticles was immersed in a DMEM–FBS cell culture medium, and then biomineralization occurred on the nanocomposite surface, which was characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. Such formed bone-like apatite on the surface facilitated the proliferation of osteoblasts, identified by Cell Counting Kit-8 analysis and fluorescent microscopy. This study verified the spontaneous biomineralization on the surface of a calcium phosphate-based nanocomposite by using a simple DMEM–FBS immersion strategy, which was promising for biomodification of bone substitutes.