Synthesis of new chitosan coated magnetic nanoparticles with surface modified with long-distanced amino groups as a support for bioligands binding

New magnetic nanoparticles based on Fe3O4 and coated with chemically modified chitosan have been prepared. The chemical modification of chitosan have been performed to give new magnetic material (Fe3O4–CS–(NH2)3) with surface rich of free amino groups distanced from the polymer chain. The amount of free primary amino groups available on the Fe3O4–CS–(NH2)3 nanoparticles surface was estimated by the ninhydrin method and evaluated to be 8.34 mM/g. Prepared nanoparticles were applied for bioligands immobilization: lipase from Candida rugosa and human serum albumin as a model protein, with high amount of immobilized bioligands (342.3 mg/g for lipase and 76.8 mg/g for albumin). & 2014 Elsevier B.V. All rights reserved.