Development of an electrochemical biosensor for determination of dopamine by gold modified poly(thiophene-3-boronic acid)-polyphenol oxidase modified electrode

Abstract The amperometric deposition of poly(thiophene-3-boronic acid) (PT3BA) in presence of glutaraldehdye and tyrosinase enzyme (PPO) on Gold nanopartilces (AuNPs) deposited glassy carbon electrode (GCE) forms a newer AuNPs-PT3BA-PPO electrode which was used to detect dopamine, a neurotransmitter. The charge transfer studies were carried out using cyclic voltammetry. Differential Pulse Voltammetry (DPV) technique was used to detect dopamine. Linear range of detection was 5 × 10−8 to 3 × 10−5 M and limit of detection was 2 × 10−8 M. The derived values for Michaelis-Menten constant (Km) and activation energy were 5.33 µM, respectively. AuNPs-PT3BA-PPO is suitability biosensor for the trace determinations of dopamine.