Mussel-inspired polydopamine-coated silk fibroin as a promising biomaterial

Silk fibroin (SF) is one of the natural biomaterials with promising and growing potential in different clinical applications such as corneal transplantation, donor site skin substitute and tympanic membrane. Some of the SFs that are extracted from mulberry silkworm do not have the arginyl–glycyl–aspartic acid (RGD) sequence for properly supporting cell adhesion and proliferation. Therefore, in the current study, polydopamine (PDA)-coated SFs were prepared to provide an RGD sequence, and the effect of PDA coating on different properties of SF was investigated. The results are also compared with those of an amniotic membrane (AM) that is a commercially available natural biomaterial for the mentioned applications. The Raman spectra showed characteristic peaks at 1581 and 1370 cm⁻¹, which demonstrate the formation of the coating layer on the surface of the films. The results showed that coating led to no significant difference in surface hydrophilicity; a smoother surface; and improved cell attachment and distribution; and a little decrease in membrane transparency, but the membrane still being transparent enough to provide vivid vision through it.