A novel hydrogen peroxide biosensor based on immobilized hemoglobin in 3D flower-like MoS2 microspheres structure

Abstract A three-dimensional flower-like MoS 2 microspheres were prepared and employed to immobilize hemoglobin (Hb) in order to fabricate a novel biosensor. The products were characterized by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The electrochemical properties of the designed Nafion/Hb/MoS 2 /GC electrode were investigated by the cyclic voltammograms (CVs). The experimental results show that MoS 2 was three-dimensional flower-like microspheres assembled by bent flakes with thickness of about several nanometers, and the diameter of these spheres was approximate 600 nm. Moreover, there were many pores with different diameters consisting of nanosheets on the surface of the MoS 2 hierarchical structures. The resulting biosensor displayed a high sensitivity, good reproducibility and long-time stability, and the wide linear range and detection limit was 20–180 μmol L −1 and 6.7 μmol L −1 for the detection of H 2 O 2 .