Blood compatibility investigation of nanofibrous PU–copper nanoparticles–avocado membrane

Wound healing is a complicated phenomenon, and it needs to be assisted properly to avoid life-threatening complications. In this present work, an electrospun nanofibrous mesh composed of polyurethane (PU), avocado extract (AC) and copper nanoparticles (CUPs) is developed and assessed for its wound healing potential. Green synthesis of CUPs was performed using aloe vera extract, and the formation of CUPs was evident in the ultraviolet/visible absorption spectrum. The developed mesh was characterized for its physicochemical and hemocompatible properties. Scanning electron micrographs showed the continuous, smooth, interconnected nanofibrous morphology of the PU–AC–CUP mesh. Also, energy-dispersive X-ray spectroscopy analysis confirmed the presence of CUPs in the mesh. Fourier transform infrared spectra indicated the addition of AC-based hydrophilic chemical groups, which enhanced the wettability of the composites. Protein adsorption studies revealed the selective adsorption of albumin rather than fibrinogen. The composite scaffold also offers a highly hemocompatible surface by delaying coagulation of blood through contact activation pathways and by limiting damage to red blood cells. Therefore, the excellent physicochemical properties, selective protein adsorption and blood compatibility of the fabricated mesh will be conducive for wound healing applications.