Macrophage membrane encapsulated with manganese doped ZIF-8 loaded quercetin alleviates acute lung injury

The mechanism of acute lung injury (ALI) is unclear and its treatment method is unsatisfactory. The aim of this study was to investigate the effects of macrophage membrane encapsulated manganese doped ZIF-8 (Mn@ZIF-8) loaded quercetin (Qu) to form M@Mn@ZIF-8@Qu on ALI. Mn@ZIF-8 was prepared by one-step method and combined with Qu to form M@Mn@ZIF-8@Qu, and macrophage membrane encapsulated Mn@ZIF-8@Qu to form M@Mn@ZIF-8@Qu by extrusion method. Lung tissues from control and ALI mice were used for RNA sequencing for the mechanism of M@Mn@ZIF-8@Qu ALI. The cercal ligation and puncture (CLP) model in vivo and lipopolysaccharide (LPS) models were used to evaluate the effects of M@Mn@ZIF-8@Qu on ALI. M@Mn@ZIF-8@Qu was successfully prepared and verify its good biocompatibility. Transmission electron microscope (TEM), potential and particle size and Fourier Transform Infrared Spectrometer (FT-IR) analysis showed that the construction of M@Mn@ZIF-8@Qu was successful has an encapsulation efficiency of 72.39 ± 3.87 % and a drug loading efficiency of 59.42 ± 3.28 %. The results of RNA-Seq showed Yes associated protein (YAP) was significantly decreased in lung tissues of ALI mice. It was also found that inhibition of YAP, the level of NLRP3 was significantly decreased. M@Mn@ZIF-8@Qu significantly alleviated ALI by inhibition the YAP/ NLRP3 signaling pathway. In conclusion, M@Mn@ZIF-8@Qu was successfully prepared for treating ALI. This study provides a new target and treatment for ALI.