Mussel-inspired nano-building block assemblies for mimicking extracellular matrix microenvironments with multiple functions
Source abstract
Abstract
Abstract The assembly of nano-building blocks is an effective way to produce artificial extracellular matrix microenvironments with hierarchical micro/nano structures. However, it is hard to assemble different types of nano-building blocks, to form composite coatings with multiple functions, by traditional layer-by-layer (LbL) self-assembly methods. Inspired by the mussel adhesion mechanism, we developed polydopamine (PDA)-decorated bovine serum albumin microspheres (BSA-MS) and nano-hydroxyapatite (nano-HA), and assembled them to form bioactive coatings with micro/nano structures encapsulating bone morphogenetic protein-2 (BMP-2). First, PDA-decorated nano-HA (nano-pHA) was obtained by oxidative polymerization of dopamine on nano-HA. Second, BMP-2-encapsulated BSA microspheres were prepared through desolvation, and then were also decorated by PDA (pBSA-MS). Finally, the nano-pHA and pBSA-MS were assembled using the adhesive properties of PDA. Bone marrow stromal cell cultures and in vivo implantation, showed that the pHA/pBSA (BMP-2) coatings can promote cell adhesion, proliferation, and benefited for osteoinductivity. PDA decoration was also applied to assemble various functional nanoparticles, such as nano-HA, polystyrene, and Fe 3 O 4 nanoparticles. In summary, this study provides a novel strategy for the assembly of biofunctional nano-building blocks, which surpasses traditional LbL self-assembly of polyelectrolytes, and can find broad applications in bioactive agents delivery or multi-functional coatings.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Polymer Surface Interaction Studies · Bone Tissue Engineering Materials · Nanoparticle-Based Drug Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Southwest Jiaotong University
Genome Research Center for Biomaterials Sichuan University
South University of Science and Technology
Sichuan University
Funding
Fundamental Research Funds for the Central Universities · Open fund of Key Lab of Advanced Technologies of Materials · NSFC · National Key Research and Development Program of China · 863 Program · Sichuan Province Youth Science and Technology Innovation Team
References (54)
Show source-provided references
1. Cell microenvironment engineering and monitoring for tissue engineering and regenerative medicine: the recent advances10.1155/2014/921905 ↗
2. Patterning biomaterials for the spatiotemporal delivery of bioactive molecules10.3389/fbioe.2016.00045 ↗
3. Cell metabolism: an essential link between cell growth and apoptosis10.1016/j.bbamcr.2010.08.011 ↗
4. Sustained release carriers used to delivery bone morphogenetic proteins in the bone healing process10.1111/j.1439-0264.2007.00824.x ↗
5. Nanoparticle-based targeted drug delivery10.1016/j.yexmp.2008.12.004 ↗
6. Self-assembled biodegradable nanoparticles and polysaccharides as biomimetic ECM nanostructures for the synergistic effect of RGD and BMP-2 on bone formation10.1038/srep25090 ↗
7. Albumin-based nanoparticles as potential controlled release drug delivery systems10.1016/j.jconrel.2011.07.031 ↗
8. Biological response of osteosarcoma cells to size-controlled nanostructured hydroxyapatite10.1177/0885328209339396 ↗
9. Stimuli-sensitive layer-by-layer (LbL) self-assembly systems: targeting and biosensory applications10.1016/j.jconrel.2012.12.033 ↗
10. Tissue integration of growth factor-eluting layer-by-layer polyelectrolyte multilayer coated implants10.1016/j.biomaterials.2010.10.052 ↗
11. Physical and thermal properties of a chitosan/alginate nanolayered PET film10.1016/j.carbpol.2010.04.043 ↗
12. A reversible wet/dry adhesive inspired by mussels and geckos10.1038/nature05968 ↗
13. Polydopamine and its derivative materials: synthesis and promising applications in energy, enviromental and biomedical fields10.1021/cr400407a ↗
14. The red and the black10.1021/ar100079y ↗
15. Durable hydrophilic surface modification for PTFE hollow fiber membranes10.1016/j.reactfunctpolym.2017.03.010 ↗
16. Electroresponsive and cell-affinitive polydopamine/polypyrrole composite microcapsules with a dual-function of on-demond drug delivery and cell stimulation for electrical therapy10.1038/am.2017.16 ↗
17. Polydopamine-mediated surface modification of scaffold materials for human neural steam cell engineering10.1016/j.biomaterials.2012.06.067 ↗
18. Bioadhesive microporous architecture by self-assembling polydopamine microcapsules for biomedical applications10.1021/cm5038765 ↗
19. Antibacterial performace of polydopamine-modified polymer surface containing passive and active components10.1021/am200978h ↗
20. Polymer brushes: mussels-inspried polymer carpets: direct photografting of polymer brushes on polydopamine nanosheets for controlled cell adhesion10.1002/adma.201670043 ↗
21. Preparation of polydopamine coated Fe3O4 nanoparticles and their application for enrichment of polycyclic aromatic hydrocarbons from environmental water samples10.1016/j.chroma.2013.01.110 ↗
22. Polydopamine-coated magnetic nanoparticles for enrichment and direct detection of small molecule pollutants coupled with MALDI-TOF-MS10.1021/am3027025 ↗
23. Facile synthesis of polydopamine-coated molecularly imprinted silica nanoparticles for protein recognition and separation10.1016/j.bios.2013.03.024 ↗
24. Mussel-inspired polydopamine: a biocompatible and ultrastable coating for nanoparticles in vivo10.1021/nn404117j ↗
25. Polydopamine-based simple and versatile surface modification of polymeric nano drug carriers10.1021/nn405809c ↗
26. Nanostructured architectures by assembling polysaccharide-coated BSA nanoparticles for biomedical application10.1002/adhm.201400684 ↗
27. Protein-affinitive polydopamine nanoparticles as an efficient surface modification strategy for versatile porous scaffolds enhancing tissue regeneration10.1002/ppsc.201500187 ↗
28. Pulse electrochemical driven rapid layer-by-layer assembly of polydopamine and hydroxyapatite nanofilms via alternative redox in situ synthesis for bone regeneration10.1021/acsbiomaterials.6b00015 ↗
29. The significant impact of polydopamine on the catalytic performance of the carried Au nanoparticles10.1039/C4CC08489F ↗
30. An integrated approach to the structure of sepia melanin. Evidence for a high proportion of degraded 5, 6-dihydroxyindole-2-carboxylic acid units in the pigment backbone10.1016/S0040-4020(97)00494-8 ↗
31. A simple equation for description of solute release: II. Fickian and anomalous release from swellable devices10.1016/0168-3659(87)90035-6 ↗
32. Osteoblastic cell adhesion on strontium-incorporated porous nanostructured TiO2 coating prepared by micro-arc oxidation10.4172/2471-8416.100007 ↗
33. Engineered nanostructured coatings for enhanced protein adsorption and cell growth10.1557/opl.2012.394 ↗
34. The nanostructure effect on the adhesion and growth rates of epithelial cells with well-defined nanoporous alumina substrates10.1088/0957-4484/21/12/125104 ↗
35. Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth10.1152/ajpheart.00199.2009 ↗
36. Delivery of a transforming growth factor β-1 plasmid to mesenchymal stem cells via cationized pleurotus eryngii polysaccharide nanoparticles10.2147/ijn.s28010 ↗
37. Preparation of nano-and microcapsules by electrophoretic polymer assembly10.1002/anie.201302092 ↗
38. Layer-by-layer self-assembly under high gravity field10.1021/la301553w ↗
39. Template-free synthesis and encapsulation technique for layer-by-layer polymer nanocarrier fabrication10.1021/nn202833n ↗
40. Highly reactive multilayer-assembled TiO2 coating on electrospun polymer nanofibers10.1002/adma.200802458 ↗
41. Chitosan-based nanoparticles as a sustained protein release carrier for tissue engineering applications10.1002/jbm.a.34031 ↗
42. Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds10.2147/IJN.S18753 ↗
43. Mussel-inspired adhesive and transferable free-standing films by self-assembling dexamethasone encapsulated BSA nanoparticles and vancomycin immobilized oxidized alginate10.1016/j.colsurfb.2014.12.050 ↗
44. Dopamine covalently modifies and functionally inactivates parkin10.1038/nm1314 ↗
45. Materials from mussel-inspired chemistry for cell and tissue engineering applications10.1021/acs.biomac.5b00852 ↗
46. Dopamine–melanin colloidal nanospheres: an efficient near-infrared photothermal therapeutic agent for in vivo cancer therapy10.1002/adma.201204683 ↗
47. Polyethylenimine-coated albumin nanoparticles for BMP-2 delivery10.1002/btpr.12 ↗
48. Nanoparticle–cell interactions: molecular structure of the protein corona and cellular outcomes10.1021/ar500190q ↗
49. Porous nano-HA/collagen/PLLA scaffold containing chitosan microspheres for controlled delivery of synthetic peptide derived from BMP-210.1016/j.jconrel.2008.11.020 ↗
50. Bone morphogenic protein-2 (BMP-2) loaded hybrid coating on porous hydroxyapatite scaffolds for bone tissue engineering10.1007/s10856-012-4822-0 ↗
51. Bone formation on apatite-coated titanium with incorporated BMP-2/heparin in vivo10.1016/j.tripleo.2009.06.039 ↗
52. Nanoparticle based inorganic coatings for corrosion protection of magnesium alloys10.1179/174329408X282550 ↗
53. Substrate independent silver nanoparticle based antibacterial coatings10.1016/j.biomaterials.2014.02.033 ↗
54. An antibacterial coating based on a polymer/sol–gel hybrid matrix loaded with silver nanoparticles10.1186/1556-276X-6-305 ↗