Fibrinogen-based cell and spheroid sheets manipulating and delivery for mouse hindlimb ischemia
Source abstract
Abstract
Abstract In this research, we introduced a novel strategy for fabricating cell sheets (CSs) prepared by simply adding a fibrinogen solution to growth medium without using any synthetic polymers or chemical agents. We confirmed that the fibrinogen-based CS could be modified for target tissue regardless of size, shape, and cell types. Also, fibrinogen-based CSs were versatile and could be used to form three-dimensional (3D) CSs such as multi-layered CSs and those mimicking native blood vessels. We also prepared fibrinogen-based spheroid sheets for the treatment of ischemic disease. The fibrinogen-based spheroid sheets had much higher in vitro tubule formation and released more angiogenic factors compared to other types of platform in this research. We transplanted fibrinogen-based spheroid sheets into a mouse hindlimb ischemia model and found that fibrinogen-based spheroid sheets showed significantly improved physiological function and blood perfusion rates compared to the other types of platform in this research.
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Research context
OpenAlex topics: Mesenchymal stem cell research · Electrospun Nanofibers in Biomedical Applications · Corneal Surgery and Treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (51)
Show source-provided references
1. Concise review: cell therapy and tissue engineering for cardiovascular disease10.5966/sctm.2012-0030 ↗
2. Stem cell-derived cell sheet transplantation for heart tissue repair in myocardial infarction10.1186/s13287-019-1536-y ↗
3. Tissue engineering based on cell sheet technology10.1002/adma.200701978 ↗
4. Effective stacking and transplantation of stem cell sheets using exogenous ROS-producing film for accelerated wound healing10.1016/j.actbio.2019.01.019 ↗
5. Temperature-responsive culture dishes allow nonenzymatic harvest of differentiated Madin-Darby canine kidney (MDCK) cell sheets10.1002/(SICI)1097-4636(200008)51:2<216::AID-JBM10>3.0.CO;2-K ↗
6. Cell sheet engineering10.1016/S1369-7021(04)00234-2 ↗
7. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide)10.1002/jbm.820271005 ↗
8. Cell and cell sheet recovery from pNIPAm coatings; motivation and history to present day approaches10.1039/c2jm31748f ↗
9. Systemically replicated organic and inorganic bony microenvironment for new bone formation generated by a 3D printing technology10.1039/C5RA20218C ↗
10. Cell culture on a thermo-responsive polymer surface10.1038/nbt0990-854 ↗
11. Cell sheet engineering: recreating tissues without biodegradable scaffolds10.1016/j.biomaterials.2005.04.061 ↗
12. Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration10.1038/s41598-020-66375-1 ↗
13. Thickness limitation and cell viability of multi-layered cell sheets and overcoming the diffusion limit by a porous-membrane culture insert10.4172/2153-0777.S1-007 ↗
14. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium10.1056/NEJMoa040455 ↗
15. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model10.1016/j.biomaterials.2009.01.032 ↗
16. Multifunctional cell-culture platform for aligned cell sheet monitoring, transfer printing, and therapy10.1021/nn5064634 ↗
17. Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction10.1038/nm1391 ↗
18. The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA10.1016/j.molcel.2009.11.033 ↗
19. Responsive systems for cell sheet detachment10.4161/org.25149 ↗
20. The evolution of vertebrate blood coagulation as viewed from a comparison of puffer fish and sea squirt genomes10.1073/pnas.0932632100 ↗
21. Fibrinogen10.1016/S1357-2725(99)00032-1 ↗
22. Gelation dynamics and gel structure of fibrinogen10.1016/j.colsurfb.2004.02.017 ↗
23. Protein source and choice of anticoagulant decisively affect nanoparticle protein corona and cellular uptake10.1039/C5NR08196C ↗
24. Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro10.14202/vetworld.2015.143-146 ↗
25. Topographically defined, biodegradable nanopatterned patches to regulate cell fate and acceleration of bone regeneration10.1021/acsami.8b14745 ↗
26. Fibrin supports human fetal islet-epithelial cell differentiation via p70s6k and promotes vascular formation during transplantation10.1038/labinvest.2015.74 ↗
27. Fibrin binds to collagen and provides a bridge for αVβ 3 integrin-dependent contraction of collagen gels10.1042/BJ20140201 ↗
28. Fibrinogen degradation product fragment D induces endothelial cell detachment by activation of cell-mediated fibrinolysis10.1172/JCI116144 ↗
29. Keratinocyte cytoskeletal roles in cell sheet engineering10.1186/1472-6750-13-17 ↗
30. Cell sheet tissue engineering: cell sheet preparation, harvesting/ manipulation, and transplantation10.1002/jbm.a.36627 ↗
31. Reconstruction of functional tissues with cell sheet engineering10.1016/j.biomaterials.2007.07.052 ↗
32. Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro10.1038/nprot.2012.027 ↗
33. Matrix nanotopography as a regulator of cell function10.1083/jcb.201108062 ↗
34. Tissue engineering of blood vessel10.1111/j.1582-4934.2007.00099.x ↗
35. Reconstruction of vascular structure with multicellular components using cell transfer printing methods10.1002/adhm.201300548 ↗
36. Capillary networks for bio-artificial three-dimensional tissues fabricated using cell sheet based tissue engineering10.3390/ijms22010092 ↗
37. Measurement system for biomechanical properties of cell sheet10.1109/iros.2013.6696474 ↗
38. Self-assembled collagen-fibrin hydrogel reinforces tissue engineered adventitia vessels seeded with human fibroblasts10.1038/s41598-018-21681-7 ↗
39. Effects of mesenchymal stem cell‐derived paracrine signals and their delivery strategies10.1002/adhm.202001689 ↗
40. Transplantation of adipose stromal cell sheet producing hepatocyte growth factor induces pleiotropic effect in ischemic skeletal muscle10.3390/ijms20123088 ↗
41. Immunomodulatory strategies for bone regeneration: a review from the perspective of disease types10.1016/j.biomaterials.2022.121604 ↗
42. Enhanced angiogenic effect of adipose-derived stromal cell spheroid with low-level light therapy in hind limb ischemia mice10.1016/j.biomaterials.2014.07.061 ↗
43. Particle-based artificial three-dimensional stem cell spheroids for revascularization of ischemic diseases10.1126/sciadv.aaz8011 ↗
44. Three-dimensional cell grafting enhances the angiogenic efficacy of human umbilical vein endothelial cells10.1089/ten.tea.2011.0193 ↗
45. Adipose-derived stem cell spheroid treated with low-level light irradiation accelerates spontaneous angiogenesis in mouse model of hindlimb ischemia10.1016/j.jcyt.2017.06.005 ↗
46. Improving the angiogenic potential of epcs via engineering with synthetic modified mRNAs10.1016/j.omtn.2018.09.005 ↗
47. A glycan-based approach to therapeutic angiogenesis10.1371/journal.pone.0182301 ↗
48. The fibrin matrix regulates angiogenic responses within the hemostatic microenvironment through biochemical control10.1371/journal.pone.0135618 ↗
49. Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications10.2147/IJN.S43945 ↗
50. Fibrin fragment E stimulates the proliferation, igration and differentiation of human microvascular endothelial cells in vitro10.1023/A:1016076121918 ↗
51. Vascular endothelial growth factor binds to fibrinogen and fibrin and stimulates endothelial cell proliferation10.1182/blood.V96.12.3772 ↗