Formation of a Giant Toroid from Long Duplex DNA
Source abstract
Abstract
It is well-known that polyamines, such as spermidine, have the effect of condensing DNA molecules into a toroidal morphology with an outer diameter of 80−100 nm. We have investigated the collapse of long duplex T4 DNA induced by spermidine with use of fluorescence and electron microscopy. We found that giant toroids with an outer diameter of ∼200 nm are generated from single T4 DNA under a high concentration of spermidine in the buffer solutions with rather high salt.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: DNA and Nucleic Acid Chemistry · Polyamine Metabolism and Applications · Bacteriophages and microbial interactions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Food and Nutrition, Nagoya Bunri College, Nagoya 451-0077, Japan, Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, and Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-0064, Japan
References (25)
Show source-provided references
1. Gosule L. C. · Nature · 1976 · 259 · 33310.1038/259333a0 ↗
2. Chattoraj D. K. · J. Mol. Biol. · 1978 · 121 · 32710.1016/0022-2836(78)90367-4 ↗
3. Plum G. E. · Biopolymers · 1990 · 30 · 63110.1002/bip.360300515 ↗
4. Sikorav J.-L. · Biophys. J. · 1994 · 67 · 138710.1016/S0006-3495(94)80640-X ↗
5. Flink I. · Nature · 1975 · 253 · 6210.1038/253062a0 ↗
6. Bloomfield V. A. · Curr. Opin. Struct. Biol. · 1996 · 6 · 33410.1016/S0959-440X(96)80052-2 ↗
7. Laemmli U. K. · Proc. Natl. Acad. Sci. U.S.A. · 1975 · 72 · 428810.1073/pnas.72.11.4288 ↗
8. Emi N. · Biochem. Biophys. Res. Commun. · 1977 · 231 · 42110.1006/bbrc.1997.6125 ↗
9. Hsiang W. M. · Proc. Natl. Acad. Sci. U.S.A. · 1977 · 74 · 485210.1073/pnas.74.11.4852 ↗
10. Lerman L. S. · Proc. Natl. Acad. Sci. U.S.A. · 1971 · 68 · 188610.1073/pnas.68.8.1886 ↗
11. Evdokimov Yu. M. · FEBS Lett. · 1972 · 23 · 18010.1016/0014-5793(72)80335-1 ↗
12. Minagawa K. · Biopolymers · 1994 · 34 · 55510.1002/bip.360340410 ↗
13. Melnikov S. M. · J. Am. Chem. Soc. · 1995 · 117 · 240110.1021/ja00114a004 ↗
14. Lang D. · J. Mol. Biol. · 1973 · 78 · 24710.1016/0022-2836(73)90113-7 ↗
15. Ueda M. · Phys. Rev. Lett. · 1996 · 77 · 213310.1103/PhysRevLett.77.2133 ↗
16. Widom J. · J. Mol. Biol. · 1980 · 144 · 43110.1016/0022-2836(80)90330-7 ↗
17. Ma C. · Biophys. J. · 1994 · 67 · 167810.1016/S0006-3495(94)80641-1 ↗
18. Yamazaki Y. · J. Am. Chem. Soc. · 1997 · 119 · 1057310.1021/ja971849r ↗
19. Arscott P. G. · Biopolymers · 1990 · 30 · 61910.1002/bip.360300514 ↗
20. Bloomfield V. A. · Biopolymers · 1991 · 31 · 147110.1002/bip.360311305 ↗
21. Yoshikawa Y. · Biophys. Chem. · 1996 · 61 · 9310.1016/S0301-4622(96)02184-9 ↗
22. Vasilevskaya V. V. · Biopolymers · 1997 · 41 · 5110.1002/(SICI)1097-0282(199701)41:1<51::AID-BIP5>3.0.CO;2-1 ↗
23. Takahashi M. · J. Phys. Chem. · 1997 · 101 · 939610.1021/jp9716391 ↗
24. Tabor C. W. · Annu. Rev. Biochem. · 1984 · 53 · 74910.1146/annurev.bi.53.070184.003533 ↗
25. Conrad M. · Proc. Natl. Acad. Sci. U.S.A. · 1989 · 86 · 970710.1073/pnas.86.24.9707 ↗