Tunable Cell-Adhesive Surfaces by Surface-Initiated Photoinduced Electron-Transfer-Reversible Addition–Fragmentation Chain-Transfer Polymerization

Cell adhesion involves many interactions between various molecules on the cell membrane (receptors, coreceptors, integrins, etc.) and surfaces or other cells. Cell adhesion plays a crucial role in the analysis of immune response, cancer treatment, tissue engineering, etc. Cell-cell adhesion can be quantified by measuring cell avidity, which defines the total interaction strength of the live cell binding. Typically, those investigations use tailor-made, reusable chips or surfaces onto which cells are cultured to form a monolayer to which other cells can bind. Cell avidity can then be measured by applying a force and quantifying cell-cell bond ruptures. The subsequent cleaning and reactivation of such biochip and biointeractive surfaces often require repeated etching, leading to device damage. Furthermore, it is often of great interest to harvest the cells that remain bound at the end of an avidity experiment for further analysis or use. It is, therefore, advantageous to pursue coating methods that allow tunable cell adhesion. This work presents temperature-switchable poly(di(ethylene glycol) methyl ether methacrylate) brush-based cell-interactive coatings produced by surface-initiated photoinduced electron-transfer reversible addition-fragmentation chain-transfer polymerization. The temperature switch of these brushes was explored by using a quartz crystal microbalance with dissipation monitoring, chemical composition, and physicochemical properties by atom force microscopy, X-ray photoelectron spectroscopy, single-molecule force spectroscopy, and ellipsometry.