Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein
Source abstract
Abstract
In this article, it is shown that by exposing commonly used lipids for biomembrane mimicking studies, to a solution containing the histidine-rich intrinsically disordered protein histatin 5, a protein cushion spontaneously forms underneath the bilayer. The underlying mechanism is attributed to have an electrostatic origin, and it is hypothesized that the observed behavior is due to proton charge fluctuations promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. Hence, we anticipate that this novel "green" approach of forming cushioned bilayers can be an important tool to mimic the cell membrane without the disturbance of the solid substrate, thereby achieving a further understanding of protein-cell interactions.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Lipid Membrane Structure and Behavior · Nanopore and Nanochannel Transport Studies · Bacteriophages and microbial interactions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Partnership for Soft Condensed Matter, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France
Department of Life and Environmental Sciences, Polytechnic University of Marche, 60131 Ancona, Italy
Division of Theoretical Chemistry, Department of Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
ISIS Pulsed Neutron Facility, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, STFC, Didcot, Oxon OX11 0QX, United Kingdom
Department of Physics and Astronomy, Uppsala University, P.O. Box 516, SE-75120 Uppsala, Sweden
LINXS—Lund Institute of Advanced Neutron and X-ray Science, Scheelevägen 19, SE-233 70 Lund, Sweden
Funding
Crafoordska Stiftelsen · NordForsk
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