Phase Behavior and Coassembly of DNA and Lysozyme in Dilute Aqueous Mixtures: A Model Investigation of DNA−Protein Interactions
Source abstract
Abstract
Results from an experimental investigation of the phase behavior of an aqueous system of DNA from salmon testes and the protein lysozyme are presented. At very low concentrations of either or both of the macromolecular components, wormlike assemblies with a width of the order of 10 nm are formed. There are strong indications that direct interactions between the protein units are instrumental both in driving the phase separation and in controlling the morphology of the formed assemblies.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Electrostatics and Colloid Interactions · Material Dynamics and Properties · Surfactants and Colloidal Systems
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Chemistry, University of Coimbra, Rua Larga, 3004-535 Coimbra, Portugal
Division of Physical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
References (11)
Show source-provided references
1. Kornberg R. D. · Nat. Struct. Mol. Biol. · 2007 · 14 · 98610.1038/nsmb1107-986 ↗
2. Holmberg K. · 200210.1002/0470856424 ↗
3. Stenstam A. · Langmuir · 2001 · 17 · 751310.1021/la011096t ↗
4. Tanford C. · J. Am. Chem. Soc. · 1954 · 76 · 333110.1021/ja01641a076 ↗
5. Lund M. · Biochemistry · 2005 · 44 · 572210.1021/bi047630o ↗
6. Korolev N. I. · Biopolymers · 1994 · 34 · 127510.1002/bip.360340915 ↗
7. Xia J. · 1994 · 24710.1007/978-3-642-78469-9_15 ↗
8. Piculell L. · Adv. Colloid Interface Sci. · 1992 · 41 · 14910.1016/0001-8686(92)80011-L ↗
9. Gaweda S. · J. Colloid Interface Sci. · 2008 · 323 · 7510.1016/j.jcis.2008.04.006 ↗
10. Hud N. V. · Annu. Rev. Biophys. Biomol. Struct. · 2005 · 34 · 29510.1146/annurev.biophys.34.040204.144500 ↗
11. Örberg M. L. · Biomacromolecules · 2007 · 8 · 155710.1021/bm061194z ↗