DataVXLangmuirPaper
journal article·2019-10-29

Importance of Polyacrylamide Hydrogel Diverse Chains and Cross-Linking Density for Cell Proliferation, Aging, and Death

Ting Wang, Guanwen Qu, Chu Wang, Yaozhong Cheng, Jing Shang, Jie Zheng, Zhangqi Feng, Qiang Chen, Nongyue He

Langmuir· Volume 35· Issue 43
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Source abstract

Abstract

'-methylenebisacrylamide (Bis)/acrylamide (Acr), we prepared polyacrylamide (PAM) hydrogels with three different polymer structures using ultraviolet irradiation. Moreover, we quantified their Flory's cross-linking densities, gel concentrations, and mechanical properties and evaluated their influence to HL-7702 liver cell behavior and metabolism. Results showed that PAM hydrogel at a ratio of Bis/Acr = 1:50 (Acr-50) owned the highest cross-linking density (0.04), which provided abundant binding sites for cell adhesion and allowed for rapid cell proliferation. On the basis of the binding sites, cells had strong traction interaction from fibrillate adhesion with the polymers, allowed easy cell migration, and induced the living cell aggregations with a diameter of 800 μm. Cells in aggregation exhibited healthy cell phenotypes and functions, and also the mitosis of the cells in aggregation is exactly the same with the cells in tissue. It is concluded that cell proliferation, aging, and death can be controlled by adjusting the cross-linking density and diverse chains of matrix hydrogels. This work will be helpful to design new functional soft biomaterials for tissue regeneration in the future.
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersBiomaterials

OpenAlex topics: 3D Printing in Biomedical Research · Hydrogels: synthesis, properties, applications · Cellular Mechanics and Interactions

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Bioelectronics, National Demonstration Centre for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu 210096, People’s Republic of China

Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, Ohio 44325, United States

School of Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing, Jiangsu 210094, People’s Republic of China

Department of Pharmacology, China Pharmaceutical University, Nanjing, Jiangsu 210002, People’s Republic of China

Nanjing Daniel New Mstar Technology, Limited, Nanjing, Jiangsu 211200, People’s Republic of China

Funding

Natural Science Foundation of Jiangsu Province · Nanjing Medical University · Southeast University · National Natural Science Foundation of China · Chinese Medical Association · State Key Laboratory of Natural Medicines, China Pharmaceutical University · Specialized Research Fund for the Doctoral Program of Higher Education · L'Oreal China

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