Impedimetric monitoring of microRNA-200c-3p using hydroxyapatite nanoparticles modified pencil graphite electrodes
Source abstract
Abstract
Abstract It is important to monitor miRNA-200c-3p as it can potentially serve as an important biomarker for respiratory diseases including COVID-19 and cancer. Despite the importance of microRNA monitoring, there are few previous studies for miRNA-200c-3p monitoring, and the application of hydroxyapatite nanoparticles (HaNP) in miRNA biosensors is quite limited. This study aims to fill this gap by utilizing the advantageous properties of HaNPs to develop a powerful strategy to detect microRNA-200c-3p. First, HaNPs were modified on the surface of pencil graphite electrodes (PGEs). Subsequently, hybridization between a phosphate-labeled miRNA-200c-3p-specific DNA probe and its complementary RNA target was carried out in the solution phase. The DNA-RNA hybrid forms were immobilized on the surface of the HaNP-PGEs and the impedimetric measurements were performed. The changes at the charge transfer resistance value (Rct) were evaluated in terms of the hybridization and optimization of the experimental conditions. The detection limits (DLs) were calculated as 0.12 µg/mL (16.19 nM) in phosphate buffer solution (PBS, pH 7.40) and 0.31 µg/mL (41.82 nM) in synthetic plasma. The selectivity of the developed biosensor was tested against miRNA-200c-5p and miRNA-141-3p. The results promise a significant improvement in public health in terms of a leap forward in the early diagnosis of many serious diseases.
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Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · MicroRNA in disease regulation · RNA Interference and Gene Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (60)
Show source-provided references
1. Yue J (2011) miRNA and vascular cell movement. Adv Drug Deliv Rev 63(8):616–62210.1016/j.addr.2011.01.001 ↗
2. Wang SS et al (2018) A meta-analysis of dysregulated miRNAs in coronary heart disease. Life Sci 215:170–18110.1016/j.lfs.2018.11.016 ↗
3. Chakraborty C et al (2020) The interplay among miRNAs, major cytokines, and cancer-related inflammation. Mol Ther Nucleic Acids 20:606–62010.1016/j.omtn.2020.04.002 ↗
4. Ghafouri-Fard S, Shoorei H, Taheri M (2020) miRNA profile in ovarian cancer. Exp Mol Pathol 113:104381
5. Juźwik CA et al (2019) microRNA dysregulation in neurodegenerative diseases: a systematic review. Prog Neurobiol 182:10166410.1016/j.pneurobio.2019.101664 ↗
6. Feng J, Xing W, Xie L (2016) Regulatory roles of microRNAs in diabetes. Int J Mol Sci 17:172910.3390/ijms17101729 ↗
7. Meng T et al (2020) An enzyme-free electrochemical biosensor based on target-catalytic hairpin assembly and Pd@UiO-66 for the ultrasensitive detection of microRNA-21. Anal Chim Acta 1138:59–6810.1016/j.aca.2020.09.022 ↗
8. Meng T et al (2020) Pd nanoparticles-DNA layered nanoreticulation biosensor based on target catalytic hairpin assembly for ultrasensitive and selective biosensing of microRNA-21. Sens Actuators B Chem 323:12862110.1016/j.snb.2020.128621 ↗
9. Ling L et al (2022) Addressing the clinical feasibility of adopting circulating miRNA for breast cancer detection, monitoring and management with artificial intelligence and machine learning platforms. Int J Mol Sci 23:1538210.3390/ijms232315382 ↗
10. Cimmino W et al (2023) Design of a printed electrochemical strip towards miRNA-21 detection in urine samples: optimization of the experimental procedures for real sample application. Anal Bioanal Chem 415:4511–452010.1007/s00216-023-04659-x ↗
11. Caratelli V et al (2022) Liquid biopsy beyond cancer: a miRNA detection in serum with electrochemical chip for non-invasive coeliac disease diagnosis. Adv NanoBiomed Res 2:220001510.1002/anbr.202200015 ↗
12. Zouari M et al (2020) Femtomolar direct voltammetric determination of circulating miRNAs in sera of cancer patients using an enzymeless biosensor. Anal Chim Acta 1104:188–19810.1016/j.aca.2020.01.016 ↗
13. Xu Y et al (2022) Tetrahedral DNA framework based CRISPR electrochemical biosensor for amplification-free miRNA detection. Biosens Bioelectron 217:114671
14. Dastidar MG et al (2023) A simple yet highly sensitive and selective aptasensor architecture for rapid and portable miRNA detection. Chem Eng J 454:140186
15. Serrano VM et al (2022) Carbon electrodes with gold nanoparticles for the electrochemical detection of miRNA 21–5p. Chemosensors 10:18910.3390/chemosensors10050189 ↗
16. Erdem A, Congur G, Eksin E (2013) Multi channel screen printed array of electrodes for enzyme-linked voltammetric detection of microRNAs. Sens Actuators B Chem 188:1089–109510.1016/j.snb.2013.07.114 ↗
17. Eksin E et al (2021) Paper-based electrode assemble for impedimetric detection of miRNA. Talanta 225:12204310.1016/j.talanta.2020.122043 ↗
18. Yarali E et al (2022) Impedimetric detection of miRNA biomarkers using paper-based electrodes modified with bulk crystals or nanosheets of molybdenum disulfide. Talanta 241:12323310.1016/j.talanta.2022.123233 ↗
19. Khodadoust A et al (2023) High-performance strategy for the construction of electrochemical biosensor for simultaneous detection of miRNA-141 and miRNA-21 as lung cancer biomarkers. Talanta 252:12385410.1016/j.talanta.2022.123863 ↗
20. Khodadoust A et al (2022) A ratiometric electrochemical DNA-biosensor for detection of miR-141. Microchim Acta 189:21310.1007/s00604-022-05301-w ↗
21. Liu Q et al (2017) miRNA-200c-3p is crucial in acute respiratory distress syndrome. Cell Discov 3:17021
22. Liu Y et al (2020) MicroRNA-200c-3p inhibits proliferation and migration of renal artery endothelial cells by directly targeting ZEB2. Exp Cell Res 387(2):111778
23. Zhang J et al (2019) MicroRNA-200c-3p/ZEB2 loop plays a crucial role in the tumor progression of prostate carcinoma. Ann Transl Med 7:141
24. Feng YL et al (2022) MicroRNA-200c-3p negatively regulates ATP2A2 and promotes the progression of papillary thyroid carcinoma. Biochem Genet 60:1676–169410.1007/s10528-022-10184-w ↗
25. Srivastava A et al (2018) A non-invasive liquid biopsy screening of urine-derived exosomes for miRNAs as biomarkers in endometrial cancer patients. AAPS J 20:1–1110.1208/s12248-018-0220-y ↗
26. Ibrahim M et al (2020) Hydroxyapatite, a multifunctional material for air, water and soil pollution control: a review. J Hazard Mater 3835:121139
27. Fihri A et al (2017) Hydroxyapatite: a review of syntheses, structure and applications in heterogeneous catalysis. Coord Chem Rev 347:48–7610.1016/j.ccr.2017.06.009 ↗
28. Kaviya M et al (2021) Synthesis and characterization of nano-hydroxyapatite/graphene oxide composite materials for medical implant coating applications. Mater Today Proc 36:204–20710.1016/j.matpr.2020.02.932 ↗
29. Chen G, Zhitomirsky I, Ghosh R (2019) Fast, low-pressure chromatographic separation of proteins using hydroxyapatite nanoparticles. Talanta 199:472–47710.1016/j.talanta.2019.02.090 ↗
30. Avila JD et al (2020) Additively manufactured Ti6Al4V-Si-hydroxyapatite composites for articulating surfaces of load-bearing implants. Addit Manuf 34:101241
31. Ma Y et al (2020) Preparation of hydroxyapatite with high surface area and dispersity templated on calcium carbonate in dipeptide hydrogels. Colloids Surf A 596:124740
32. Sughanthy SAP, Ansari MNM, Atiqah A (2020) Dynamic mechanical analysis of polyethylene terephthalate/hydroxyapatite biocomposites for tissue engineering applications. J Mater Res Technol 2:2350–235610.1016/j.jmrt.2019.12.066 ↗
33. Shen G, Cai C, Yang J (2011) Fabrication of an electrochemical immunosensor based on a gold–hydroxyapatite nanocomposite–chitosan film. Electrochim Acta 56:8272–827710.1016/j.electacta.2011.06.090 ↗
34. Wang S et al (2010) Hydroxyapatite nanoarray-based cyanide biosensor. Anal Biochem 398:191–19710.1016/j.ab.2009.11.029 ↗
35. Kanchana P, Sekar C (2015) Development of electrochemical folic acid sensor based on hydroxyapatite nanoparticles. Spectrochim Acta Part A Mol Biomol Spectrosc 137:58–6510.1016/j.saa.2014.07.087 ↗
36. Kanchana P, Navaneethan M, Sekar C (2017) Fabrication of Ce doped hydroxyapatite nanoparticles based non-enzymatic electrochemical sensor for the simultaneous determination of norepinephrine, uric acid and tyrosine. Mater Sci Eng B 226:132–14010.1016/j.mseb.2017.09.015 ↗
37. Kanchana P, Lavanya N, Sekar C (2014) Development of amperometric L-tyrosine sensor based on Fe-doped hydroxyapatite nanoparticles. Mater Sci Eng C 35:85–9110.1016/j.msec.2013.10.013 ↗
38. Yang Z, Zhang C (2011) Molecularly imprinted hydroxyapatite thin film for bilirubin recognition. Biosens Bioelectron 29:167–17110.1016/j.bios.2011.08.012 ↗
39. Yang Z et al (2014) Photoelectrochemical bilirubin biosensor based on Fe3O4/hydroxyapatite/molecularly imprinted polypyrrole nanoparticles. Sens Actuators B Chem 201:167–17210.1016/j.snb.2014.05.021 ↗
40. Shahamirifard SA, Ghaedi M (2019) A new electrochemical sensor for simultaneous determination of arbutin and vitamin C based on hydroxyapatite-ZnO-Pd nanoparticles modified carbon paste electrode. Biosens Bioelectron 141:11147410.1016/j.bios.2019.111474 ↗
41. Erdem A, Congur G (2018) Hydroxyapatite nanoparticles modified graphite electrodes for electrochemical DNA detection. Electroanalysis 30:67–7410.1002/elan.201700462 ↗
42. Hend S et al (2021) Electrochemical impedance spectroscopy (EIS): principles, construction, and biosensing applications. Sensors 21:6578
43. Cho H et al (2022) Electrochemical impedance-based biosensors for the label-free detection of the nucleocapsid protein from SARS-CoV-2. ACS Sens 7:1676–168410.1021/acssensors.2c00317 ↗
44. Chawla K et al (2018) Impedance-based microfluidic assay for automated antischistosomal drug screening. ACS Sens 3:2613–262010.1021/acssensors.8b01027 ↗
45. Meng T et al (2020) Construction of an ultrasensitive electrochemical sensing platform for microRNA-21 based on interface impedance spectroscopy. J Colloid Inter Sci 578:164–17010.1016/j.jcis.2020.05.118 ↗
46. Teengam P et al (2018) Electrochemical impedance-based DNA sensor using pyrrolidinyl peptide nucleic acids for tuberculosis detection. Anal Chim Acta 1044:102–10910.1016/j.aca.2018.07.045 ↗
47. El-Sheikh SM et al (2021) A novel Ag/Zn bimetallic MOF as a superior sensitive biosensing platform for HCV-RNA electrochemical detection. App Surf Sci 562:150202
48. Miller JN, Miller JC (2005) Statistics and chemometrics for analytical chemistry. Pearson Education, London, p 121–125
49. Pang SN et al (2022) Urinary microRNA sensing using electrochemical biosensor to evaluate colorectal cancer progression. Biomedicines 10:1434
50. Ouyang R et al (2023) Ti3C2@Bi2O3 nanoaccordion for electrochemical determination of miRNA-21. Microchim Acta 190:5210.1007/s00604-022-05624-8 ↗
51. Chai H et al (2021) Ultrasensitive electrochemical detection of miRNA coupling tetrahedral DNA modified gold nanoparticles tags and catalyzed hairpin assembly. Anal Chim Acta 1165:33854310.1016/j.aca.2021.338543 ↗
52. Liu S et al (2023) A novel detection of MicroRNA based on homogeneous electrochemical sensor with enzyme-assisted signal amplification. Talanta 256:124263
53. Wang Q et al (2021) Ultrasensitive electrochemical detection of miRNA based on polymerization signal amplification. Talanta 235:122744
54. Zhang Q, Guo Z, Zheng X (2023) Synthesis of Ag@carbonized polymer dots and their electrochemical sensing of miRNA. Electroanalysis 35:2200190
55. Zheng Y et al (2021) Dual-mode biosensor for femtomolar miRNA-155 detection by electrochemiluminescence and adsorptive stripping voltammetry. Microchem J 165:10609110.1016/j.microc.2021.106091 ↗
56. Hu M et al (2021) CoNi bimetallic metal–organic framework as an efficient biosensing platform for miRNA 126 detection. App Surf Sci 542:148586
57. Vural OA et al (2021) Human serum albumin–gold nanoparticle based impedimetric sensor for sensitive detection of miRNA-200c. Electroanalysis 33:925–93510.1002/elan.202060342 ↗
58. Li D et al (2019) Exosomes from miR-21-5p-increased neurons play a role in neuroprotection by suppressing rab11a-mediated neuronal autophagy in vitro after traumatic brain injury. Med Sci Monit 25:1871–188510.12659/MSM.915727 ↗
59. Chen Z et al (2023) Specific adsorption of magnetic Fe3O4@hydroxyapatite nanocomposites to a hybridized duplex for immobilization and label-free electrochemical biosensing of microRNA. ACS Appl Nano Mater 6(10):8584–859210.1021/acsanm.3c00951 ↗
60. Kuang Y et al (2019) Duplex-specific nuclease-mediated amplification strategy for mass spectrometry quantification of miRNA-200c in breast cancer stem cells. Anal Chem 91:8820–882610.1021/acs.analchem.8b04468 ↗