DataVXJournal of Materials SciencePaper
journal article·2021-03

Capture of DNAs by magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres

Maria Fecková, Judit Tóth, Petr Šálek, Alena Španová, Daniel Horák, Quazi T. H. Shubhra, Aleš Kovařík, János Gyenis, Bohuslav Rittich

Journal of Materials Science· Volume 56· Issue 9
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Abstract

Magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres (mgt.HPS1-NH2 and mgt.HPS2-NH2) containing different contents of amino groups were prepared and characterized in this study. The microspheres were used for the capture of uncompacted and compacted bacterial and calf thymus DNAs in the presence of different PEG 6000 and NaCl concentrations. Magnetic macroporous poly(glycidyl methacrylate-co-[2-(methacryloyloxy)ethoxy]acetic acid-co-ethylene dimethacrylate) microspheres containing amino and carboxyl groups [mgt.P(GMA-MOEAA-EDMA)-NH2] and magnetic non-porous carboxyl group-functionalized poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [mgt.P(HEMA-GMA)-COOH] microspheres were used as a control. The size changes of uncompacted and compacted bacterial and calf thymus DNAs were measured in the range of concentrations 0–16% PEG 6000 and 0.5–2.0 M NaCl by dynamic light scattering (DLS). The highest capture of compacted bacterial and calf thymus DNAs was achieved with mgt.HPS1-NH2, mgt.HPS2-NH2 microspheres using 8% PEG 6000 and 2.0 M NaCl and with mgt.P(GMA-MOEAA-EDMA)-NH2 microspheres using 8% and 16% PEG 6000 and 2.0 M NaCl. Developed microspheres were used for DNA isolation from real vegetable (broccoli flowering head) samples. DNA was in quality suitable for PCR.
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OpenAlex topics: Biopolymer Synthesis and Applications · RNA Interference and Gene Delivery · Microfluidic and Capillary Electrophoresis Applications

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References (71)

Show source-provided references
1. Horák D, Babič M, Macková H, Beneš MJ (2007) Preparation and properties of magnetic nano- and microsized particles for biological and environmental separations. J Sep Sci 30:1751–1772. https://doi.org/10.1002/jssc.20070008810.1002/jssc.200700088 ↗
2. Wu Y, Zhang H, Han J, Wang W, Ju X, Chen Y (2011) PCR-CE-SSCP applied to detect cheap oil blended in olive oil. Eur Food Res Technol 233:313–332. https://doi.org/10.1007/s00217-011-1520-y10.1007/s00217-011-1520-y ↗
3. Yu Q, Zhai L, Bie X, Lu Z, Zhang C, Tao T, Li J, Lv F, Zhao H (2016) Survey of five food-borne pathogens in commercial cold food dishes and their detection by multiplex PCR. Food Control 59:862–869. https://doi.org/10.1016/j.foodcont.2015.06.02710.1016/j.foodcont.2015.06.027 ↗
4. Trojánek Z, Kovařík A, Španová A, Marošiová K, Horák D, Rittich B (2018) Application of magnetic polymethacrylate-based microspheres for the isolation of DNA from raw vegetables and processed foods of plant origin. J Food Process Pres 42:1–8. https://doi.org/10.1111/jfpp.1338410.1111/jfpp.13384 ↗
5. Aboul-Maaty NAF, Oraby HAS (2019) Extraction of high-quality genomic DNA from different plant orders applying a modified CTAB-based method. Bull Natl Res Cent. https://doi.org/10.1186/s42269-019-0066-110.1186/s42269-019-0066-1 ↗
6. Yousem SA (2012) Role of molecular studies in the diagnosis of lung adenocarcinoma. Mod Pathol 25:S11–S17. https://doi.org/10.1038/modpathol.2011.15610.1038/modpathol.2011.156 ↗
7. Binefa G, Rodríguez-Moranta F, Teule A, Medina-Hayas M (2014) Colorectal cancer: from prevention to personalized medicine. World J Gastroenterol 20:6786–6808. https://doi.org/10.3748/wjg.v20.i22.678610.3748/wjg.v20.i22.6786 ↗
8. Frimpong M, Ahor HS, Sakyi SA, Agbavor B, Akowuah E, Phillips RO (2019) Rapid extraction method of Mycobacterium ulcerans DNA from clinical samples of suspected Buruli ulcer patiens. Diagnostics 9(4):204. https://doi.org/10.3390/diagnostics904020410.3390/diagnostics9040204 ↗
9. Trachtová Š, Obermajer T, Španová A, Matijašić BB, Rogelj I, Horák D, Rittich B (2012) Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from feaces. Mol Cryst Liq Cryst 555:263–270. https://doi.org/10.1080/15421406.2012.63555410.1080/15421406.2012.635554 ↗
10. Hart ML, Meyer A, Johnson PJ, Ericsson AC (2015) Comparative Evaluation of DNA extraction methods from feces of multiple host species for downstream next-generation sequencing. PLoS ONE 10(11):e0143334. https://doi.org/10.1371/journal.pone.014333410.1371/journal.pone.0143334 ↗
11. Lim MY, Song EJ, Kim SH, Lee J, Nam YD (2018) Comparison of DNA extraction methods for human gut microbial community profilig. Syst Appl Microbiol 41:151–157. https://doi.org/10.1016/j.syapm.2017.11.00810.1016/j.syapm.2017.11.008 ↗
12. Adler CJ, Haak W, Donlon D, Cooper A (2011) Survival and recovery of DNA from ancient teeth and bones. J Archaeol Sci 38:956–964. https://doi.org/10.1016/j.jas.2010.11.01010.1016/j.jas.2010.11.010 ↗
13. Brzobohatá K, Drozdová E, Smutný J, Zeman T, Beňuš R (2017) Comparison of suitability of the most common ancient DNA quantification methods. Genet Test Mol Biomark 21:265–271. https://doi.org/10.1089/gtmb.2016.019710.1089/gtmb.2016.0197 ↗
14. Rittich B, Španová A (2013) SPE and purification of DNA using magnetic particles. J Sep Sci 36:2472–2485. https://doi.org/10.1002/jssc.20130033110.1002/jssc.201300331 ↗
15. Sen T, Bruce IJ (2006) Mesoporous silica–magnetite anocomposites: fabrication, characterisation and applications in biosciences. Microporous Mesoporous Mater 120:246–251. https://doi.org/10.1021/ja061393q10.1021/ja061393q ↗
16. Vandeventer PE, Lin JS, Zwang TJ, Nadim A, Johal MS, Niemz A (2012) Multiphasic DNA adsorption to silica surfaces under varying buffer, pH, and ionic strength conditions. J Phys Chem B 116:5661–5670. https://doi.org/10.1021/jp301777610.1021/jp3017776 ↗
17. Günal G, Kip Ç, Eda Öğüt S, İlhan H, Kibar G, Tuncel A (2017) Comparative DNA isolation behaviours of silica and polymer based sorbents in batch fashion: monodisperse silica microspheres with bimodal pore size distribution as a new sorbent for DNA isolation. Artif Cells Nanomed Biotechnol 22:1–7. https://doi.org/10.1080/21691401.2017.130440410.1080/21691401.2017.1304404 ↗
18. Moeller JR, Moehn NR, Waller DM, Givnish TJ (2014) Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa. Appl Plant Sci 2(10):1400048. https://doi.org/10.3732/apps.140004810.3732/apps.1400048 ↗
19. Lodha A, Ansari N, Shah S, Rao MV, Menon SK (2017) Isolation of PCR ready-human DNA using copper nanoparticles from skeletal remains. Forensic Sci Int 270:146–152. https://doi.org/10.1016/j.forsciint.2016.12.00310.1016/j.forsciint.2016.12.003 ↗
20. Šálek P, Korecká L, Horák D, Petrovský E, Kovářová J, Metelka R, Čadková M, Bílková Z (2011) Immunomagnetic sulfonated hypercrosslinked polystyrene microspheres for electrochemical detection of proteins. J Mater Chem 21(38):14783–14792. https://doi.org/10.1039/C1JM12475G10.1039/C1JM12475G ↗
21. Šálek P, Horák D, Hromádková J (2018) Preparation of monodisperse poly(styrene-co-divinylbenzene) microspheres by controlled dispersion polymerization. Polym Sci Ser B. 60:9–15. https://doi.org/10.1134/S156009041801011610.1134/S1560090418010116 ↗
22. Šálek P, Horák D, Bláha M (2013) PEG-modified magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres to minimize sorption of serum proteins. React Funct Polym 73:1122–1129. https://doi.org/10.1016/j.reactfunctpolym.2013.05.01110.1016/j.reactfunctpolym.2013.05.011 ↗
23. Trachtová Š, Španová A, Tóth J, Prettl Z, Horák D, Gyenis J, Rittich B (2015) Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres. Food Bioprod Process 9:375–381. https://doi.org/10.1016/j.fbp.2014.05.00110.1016/j.fbp.2014.05.001 ↗
24. Li S, Wang L, Zhao S, Lin J, Zheng J, Lin Z (2015) Preparation of phenyl-functionalized magnetic mesoporous silica microspheres for the fast separation and selective enrichment of phenyl-containing peptides. J Sep Sci 38:3954–3960. https://doi.org/10.1002/jssc.20150087610.1002/jssc.201500876 ↗
25. Li Y, Deng C, Sun N (2018) Hydrophilic probe in mesoporous pore for selective enrichment of endogenous glycopeptides in biological samples. Anal Chim Acta 1024:84–92. https://doi.org/10.1016/j.aca.2018.04.03010.1016/j.aca.2018.04.030 ↗
26. Zadeh N, Åkerman B (2017) Immobilization of enzymes in mesoporous silica particles: protein concentration and rotational mobility in the pores. J Phys Chem B 121:2575–2583. https://doi.org/10.1021/acs.jpcb.7b0056210.1021/acs.jpcb.7b00562 ↗
27. Walcarius A (2015) Mesoporous materials-based electrochemical sensors. Electroanalysis 27:1303–1340. https://doi.org/10.1002/elan.20140062810.1002/elan.201400628 ↗
28. Li S, Nguyen L, Xiong H, Wang M, Hu TC-C, She JX, Serkiz SM, Wicks GG, Dynan WS (2010) Porous-wall hollow glass microspheres as novel potential nanocarriers for biomedical applications. Manomed Nanotechnol Biol Med 6:127–136. https://doi.org/10.1016/j.nano.2009.06.00410.1016/j.nano.2009.06.004 ↗
29. Sedlačík T, Proks V, Šlouf M, Dušková-Smrčková M, Studenovská H, Rypáček F (2015) Macroporous biodegradable cryogels of synthetic poly(alpha.-amino acids). Biomacromolecules 16:3455–3465. https://doi.org/10.1021/acs.biomac.5b0122410.1021/acs.biomac.5b01224 ↗
30. Li X, Zhang J, Gu H (2012) Study on the adsorption mechanism of DNA with mesoporous silica nanoparticles in aqueous solution. Langmuir 28:2827–2834. https://doi.org/10.1021/la204443j10.1021/la204443j ↗
31. Pérez-Esteve É, Ruiz-Rico M, Martínez-Máñez R, Barat JM (2015) Mesoporous silica-based supports for the controlled and targeted release of bioactive molecules in the gastrointestinal tract. J Food Sci 80:2504–2516. https://doi.org/10.1111/1750-3841.1309510.1111/1750-3841.13095 ↗
32. Trofimov AD, Ivanova AA, Zyuzin MV, Timin AS (2018) Porous inorganic carriers based on silica, calcium carbonate and calcium phosphate for controlled/modulated drug delivery: fresh outlook and future perspectives. Pharmaceutics 10:167–236. https://doi.org/10.3390/pharmaceutics1004016710.3390/pharmaceutics10040167 ↗
33. Record MT Jr (1975) Effects of Na+ and Mg++ ions on the helix-coil transition of DNA. Biopolymers 14:2137–2158. https://doi.org/10.1002/bip.1975.36014101210.1002/bip.1975.360141012 ↗
34. Frank-Kamenetskii MD (1987) DNA chemistry How the double helix breathes. Nature 328:17–18. https://doi.org/10.1021/la000993e10.1021/la000993e ↗
35. Bloomfield VA (1997) DNA condensation by multivalent cations. Biopolymers 44:269–282. https://doi.org/10.1002/(SICI)1097-0282(1997)44:3%3c269::AID-BIP6%3e3.0.CO;2-T10.1002/(SICI)1097-0282(1997)44:3<269::AID-BIP6>3.0.CO;2-T ↗
36. Lis JT (1980) Fractionation of DNA fragments by polyethylene glycol induced precipitation. Meth Enzymol 65:347–353. https://doi.org/10.1016/S0076-6879(80)65044-710.1016/S0076-6879(80)65044-7 ↗
37. He Z, Zhu Y, Gu H (2013) A new method for the determination of critical polyethylene glycol concentration for selective precipitation of DNA fragments. Appl Microbiol Biotechnol 97:9175–9183. https://doi.org/10.1007/s00253-013-5195-010.1007/s00253-013-5195-0 ↗
38. Murphy JC, Fox GE, Willson RC (2003) Enhancement of anion- exchange chromatography of DNA using compaction agents. J Chromatogr A 984:215–221. https://doi.org/10.1016/s0021-9673(02)01814-910.1016/s0021-9673(02)01814-9 ↗
39. Murphy JC, Cano T, Fox GE, Willson RC (2006) Compaction agent protection of nucleic acids during mechanical lysis. Biotechnol Prog 22:519–522. https://doi.org/10.1021/bp050422i10.1021/bp050422i ↗
40. Kawakita H, Uneyama T, Kojima M, Morishima K, Masubuchi Y, Watanabe H (2009) Formation of globules and aggregates of DNA chains in DNA/polyethylene glycol/monovalent salt aqueous solutions. J Chem Phys 131(9):094901. https://doi.org/10.1063/1.321611010.1063/1.3216110 ↗
41. Vasilevskaya VV, Khokhlov AR, Matsuzawa Y, Yoshikawa K (1995) Collapse of single DNA molecule in poly(ethylene glycol) solutions. J Chem Phys 102:6595–6602. https://doi.org/10.1063/1.46937510.1063/1.469375 ↗
42. Kleideiter G, Nordmeier E (1999) Poly(ethylene glycol)-induced DNA condensation in aqueous/metanol containing low-molecular-weight electrolyte solutions Part I. Theor Consid, Polym 40:4013–4023. https://doi.org/10.1016/S0032-3861(98)00643-010.1016/S0032-3861(98)00643-0 ↗
43. Kleideiter G, Nordmeier E (1999) Poly(ethylene glycol)-induced DNA condensation in aqueous/methanol containing low-molecular-weight electrolyte solutions. Part II. Comp Between Exp Theor, Polym 40:4025–4033. https://doi.org/10.1016/S0032-3861(98)00644-210.1016/S0032-3861(98)00644-2 ↗
44. Kubínová Š, Horák D, Syková E (2009) Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering. Biomaterials 30:4601–4609. https://doi.org/10.1016/j.biomaterials.2009.05.00710.1016/j.biomaterials.2009.05.007 ↗
45. Overberger CG, O’Shaughnessy T, Shalit H (1949) The preparation of some aliphatic azo nitriles and their decomposition in solution. J Am Chem Soc 7:2661–266610.1021/ja01176a018 ↗
46. Horák D, Svobodová Z, Autebert J, Coudert B, Plichta Z, Královec K, Bílková Z, Viovy J-L (2013) Albumincoated monodisperse magnetic poly(glycidyl methacrylate) microspheres with immobilized antibodies: Application to the capture of epithelial cancer cells. J Biomed Mater Res Part A 101A:23–32. https://doi.org/10.1002/jbm.a.3429710.1002/jbm.a.34297 ↗
47. Horák D, Rittich B, Španová A (2007) Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products. J Magn Magn Mater 311:249–254. https://doi.org/10.1016/j.jmmm.2006.10.115710.1016/j.jmmm.2006.10.1157 ↗
48. Rigby SP, Barwick D, Fletcher RS, Riley SN (2003) Interpreting mercury porosimetry data for catalyst supports using semi-empirical alternatives to the Washburn equation. Appl Catal A Gen 238:303–318. https://doi.org/10.1016/S0926-860X(02)00348-410.1016/S0926-860X(02)00348-4 ↗
49. Sinden RR (1994) DNA structure and function academic press. Gulf Professional Publishing, San Diego
50. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018. https://doi.org/10.1073/pnas.81.24.801410.1073/pnas.81.24.8014 ↗
51. Lim KY, Kovařík A, Matyášek R, Bezděk M, Lichtenstein CP, Leitch AR (2000) Gene conversion of ribosomal DNA in Nicotiana tabacum is associated with undermethylated, decondensed and probably active gene units. Chromosoma 109:161–172. https://doi.org/10.1007/s00412005042410.1007/s004120050424 ↗
52. Kovařík A, Pires JC, Leitch AR, Lim KY, Sherwood AM, Matyášek R, Rocca J, Soltis DE, Soltis PS (2005) Rapid concerted evolution of nuclear ribosomal DNA in two tragopogon allopolyploids of recent and recurrent origin. Genetics 169:931–944. https://doi.org/10.1534/genetics.104.03283910.1534/genetics.104.032839 ↗
53. Trachtová Š, Zapletalová H, Španová A, Horák D, Kolářová H, Rittich B (2015) The evaluation of magnetic polymethacrylate-based microspheres used for solid phase DNA micro-extraction. Chromatography 2:156–166. https://doi.org/10.3390/chromatography202015610.3390/chromatography2020156 ↗
54. Cheng C, Jia JL, Ran SY (2015) Polyethylene glycol and divalent salt-induced DNA reentrant condensation revealed by single molecule measurements. Soft Matter 11:3927–3935. https://doi.org/10.1039/c5sm00619h10.1039/c5sm00619h ↗
55. Lerman LS (1971) A transition to a compact form of DNA in polymer solutions. Proc Natl Acad Sci USA 68:1886–1890. https://doi.org/10.1073/pnas.68.8.188610.1073/pnas.68.8.1886 ↗
56. Yoshikawa K (2001) Controlling the higher-order structure of giant DNA molecules. Adv Drug Deliv Rev 52:235–244. https://doi.org/10.1016/s0169-409x(01)00210-110.1016/s0169-409x(01)00210-1 ↗
57. Kojima M, Kubo K, Yoshikawa K (2006) Elongation/compaction of giant DNA caused by depletion interaction with a flexible polymer. J Chem Phys 124:2. https://doi.org/10.1063/1.214575210.1063/1.2145752 ↗
58. Minagawa K, Matsuzawa Y, Yoshikawa K, Khokhlov AR, Doi M (1994) Direct observation of the coil-globule transition in DNA molecules. Biopolymers 34:555–558. https://doi.org/10.1002/bip.36034041010.1002/bip.360340410 ↗
59. Bravo-Anaya LM, Rinaudo M, Martínez FAS (2016) Conformation and rheological properties of calf-thymus DNA in solution. Polymers 8:51. https://doi.org/10.3390/polym802005110.3390/polym8020051 ↗
60. Laesecke A, Burger JL (2014) Viscosity measurements of DNA solutions with and without condensing agents. Biorheology 51:15–28. https://doi.org/10.3233/BIR-14064910.3233/BIR-140649 ↗
61. Fujiwara M, Yamamoto F, Okamoto K, Shiokawa K, Nomura R (2005) Adsorption of duplex DNA on mesoporous silicas: possibility of inclusion of DNA into their mesopores. Anal Chem 77:8138–8145. https://doi.org/10.1021/ac051663810.1021/ac0516638 ↗
62. Tanaka T, Sakai R, Kobayashi R, Hatakeyama K, Matsunaga T (2009) Contributions of phosphate to DNA adsorption/desorption behaviors on aminosilane-modified magnetic nanoparticles. Langmuir 25:2956–2961. https://doi.org/10.1021/la803239710.1021/la8032397 ↗
63. Shan Z, Jiang Y, Guo M, Bennett JC, Li X, Tian H, Oakes K, Zhang X, Zhou Y, Huang Q, Chen H (2015) Promoting DNA loading on magnetic nanoparticles using a DNA condensation strategy. Coll Surf B Biointerfaces 125:247–254. https://doi.org/10.1016/j.colsurfb.2014.10.03610.1016/j.colsurfb.2014.10.036 ↗
64. Porsch B, Laga R, Horský J, Koňák Č, Ulbrich K (2009) Molecular weight and polydispersity of calf-thymus DNA: static light-scattering and size-exclusion chromatography with dual detection. Biomacromolecules 10:3148–3150. https://doi.org/10.1021/bm900768j10.1021/bm900768j ↗
65. Stevens MJ (2001) Simple simulations of DNA condensation. Biophys J 80:130–139. https://doi.org/10.1016/S0006-3495(01)76000-610.1016/S0006-3495(01)76000-6 ↗
66. Zhang X, Huang PJ, Servos MR, Liu J (2012) Effects of polyethylene glycol on DNA adsorption and hybridization on gold nanoparticles and graphene oxide. Langmuir. 28:14330–14337. https://doi.org/10.1021/la302799s10.1021/la302799s ↗
67. Arakawa T, Timasheff SN (1985) Mechanism of poly(ethylene glycol) interaction with proteins. Biochemistry 24:6756–676210.1021/bi00345a005 ↗
68. Hirano A, Shiraki K, Arakawa T (2012) Polyethylene glycol behaves like weak organic solvent. Biopolymers 97:117–122. https://doi.org/10.1002/bip.2170810.1002/bip.21708 ↗
69. Eskilsson K, Leal C, Lindman B, Miguel M, Nylander T (2001) DNA-surfactant complexes at solid surfaces. Langmuir 17:1666–1669. https://doi.org/10.1021/la000993e10.1021/la000993e ↗
70. Španová A, Horák D, Soudková E, Rittich B (2004) Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reactions applications. J Chromatogr B 800:27–3210.1016/j.jchromb.2003.09.010 ↗
71. Schrader C, Schielke A, Ellerbroek L, Johne R (2012) PCR inhibitors - occurrence, properties and removal. J Appl Microbiol 113:1014–1026. https://doi.org/10.1111/j.1365-2672.2012.05384.x10.1111/j.1365-2672.2012.05384.x ↗