Preparation of an enzyme/inorganic nanosheet/magnetic bead complex and its enzymatic activity
Source abstract
Abstract
A complex composed of enzyme (horseradish peroxidase, HRP), layered titanate (TiOx), and magnetic beads (MB) was prepared, and the enzymatic activity of the complex and the repeatable use for the enzyme in the complex were investigated. The HRP/TiOx/MB complex was formed in acetate buffer (pH 4) by electrostatic interaction among the components where HRP and MB have positive charge, whereas TiOx is negatively charged. The resultant complex was sufficiently stable in an aqueous solution, and HRP immobilized on the layered titanate in the complex kept a certain enzymatic activity in which typical enzymatic relationship was observed between substrate concentration and initial reaction rate. By giving a magnetic field using commercial magnets, the HRP/TiOx/MB complex was easily and rapidly recovered, and the recovered complex was able to be used for the next enzymatic reactions. The enzyme/inorganic nanosheet/MB complex proposed in this study is expected to be applied as a new tool in the bioengineering and nanobiotechnological fields.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanomaterials for catalytic reactions · Polymer Surface Interaction Studies · Layered Double Hydroxides Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (30)
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1. Gubitz G, Kunssberg E, van Zoonen P, Jansen H, Gooijer C, Velthorst NH, Fei RW (1988) In: Leyden DE, Collins WT (eds) Chemically modified surfaces, vol 2. Gordon and Breach, London
2. Hertzberg S, Kvittingen L, Anthonsen T, Skjåk-Bræk G (1992) Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: application in lipase-catalysed reactions. Enzyme Microb Technol 14:42–4710.1016/0141-0229(92)90024-I ↗
3. Cao L, Bornscheuer UT, Schmid RD (1999) Lipase-catalyzed solid-phase synthesis of sugar esters. Influence of immobilization on productivity and stability of the enzyme. J Mol Catal B 6:279–28510.1016/S1381-1177(98)00083-6 ↗
4. Katchalski-Katzir E, Kraemer DM (2000) Eupergit® C, a carrier for immobilization of enzymes of industrial potential. J Mol Catal B 10:157–17610.1016/S1381-1177(00)00124-7 ↗
5. Reetz MT, Zonta A, Simpelkamp J (1996) Efficient immobilization of lipase by entrapment in hydrophobic sol–gel materials. Biotechnol Bioeng 49:527–53410.1002/(SICI)1097-0290(19960305)49:5<527::AID-BIT5>3.3.CO;2-E ↗
6. Nguyen DT, Smit M, Dunn B, Zink JI (2002) Stabilization of creatine kinase encapsulated in silicate sol–gel materials and unusual temperature effects on its activity. Chem Mater 14:4300–430610.1021/cm020398t ↗
7. Yoshinaga K, Kondo K, Kondo A (1997) Capabilities of polymer-modified monodisperse colloidal silica particles as biomaterial carrier. Colloid Polym Sci 275:220–22610.1007/s003960050075 ↗
8. Jeng F-Y, Lin S-C (2006) Characterization and application of PEGylated horseradish peroxidase for the synthesis of poly (2-naphthol). Process Biochem 41:1566–157310.1016/j.procbio.2006.02.021 ↗
9. Kumar CV, McLendon GL (1997) Nanoencapsulation of cytochrome c and horseradish peroxidase at the galleries of α-zirconium phosphate. Chem Mater 9:863–87010.1021/cm960634y ↗
10. Kumar CV, Chaudhari A (2000) Proteins immobilized at the galleries of layered α-zirconium phosphate: structure and activity studies. J Am Chem Soc 122:830–83710.1021/ja993310u ↗
11. Wang Q, Gao Q, Shi J (2004) Enhanced catalytic activity of hemoglobin in organic solvents by layered titanate immobilization. J Am Chem Soc 126:14346–1434710.1021/ja047224s ↗
12. Wang Q, Gao Q, Shi J (2004) Reversible intercalation of large-capacity hemoglobin into in situ prepared titanate interlayers with enhanced thermal and organic medium stabilities. Langmuir 20:10231–1023710.1021/la048602+ ↗
13. Zhou Q, Lu Z, Cao X (2014) Heterostructured magnetite–titanate nanosheets for prompt charge selective binding and magnetic separation of mixed proteins. J Colloid Interface Sci 415:48–5610.1016/j.jcis.2013.10.012 ↗
14. Takahashi H, Li B, Sasaki T, Miyazaki C, Kajino T, Inagaki S (2000) Catalytic activity in organic solvents and stability of immobilized enzymes depend on the pore size and surface characteristics of mesoporous silica. Chem Mater 12:3301–330510.1021/cm000487a ↗
15. Huang S-H, Juang R-S (2011) Biochemical and biomedical applications of multifunctional magnetic nanoparticles: a review. J Nanopart Res 13:4411–443010.1007/s11051-011-0551-4 ↗
16. Oh J-M, Choi S-J, Lee G-E, Han S-H, Choy J-H (2009) Inorganic drug-delivery nanovehicle conjugated with cancer-cell-specific ligand. Adv Funct Mater 19:1617–162410.1002/adfm.200801127 ↗
17. Choy J-H, Kwak S-Y, Park J-S, Jeong Y-J, Portier J (1999) Intercalative nanohybrids of nucleoside monophosphates and DNA in layered metal hydroxide. J Am Chem Soc 121:1399–140010.1021/ja981823f ↗
18. Ida S, Araki U, Unal U, Izawa K, Altuntasoglu O, Ogata C, Matsumoto Y (2006) Photoluminescence properties of multilayer oxide films intercalated with rare earth ions by the layer-by-layer technique. Chem Commun 3619–362110.1039/b604451d ↗
19. Cao X, Xue X, Zhu L, Chen P, Song Y, Chen M (2010) Ultrasensitive Na+ exchanging performance of free-standing Fe3O4 @Na2Ti3O7 nanosheets indicated by fluorescein. J Mater Chem 20:2322–232810.1039/b921304j ↗
20. Sović D, Gajović A, Iveković D (2011) Bioelectrocatalytic and biosensing properties of horseradish peroxidase covalently immobilized on (3-aminopropyl)trimethoxysilane-modified titanate nanotube. Electrochim Acta 56:9953–996010.1016/j.electacta.2011.08.077 ↗
21. Kang XB, Pang GC, Liang XY, Wang M, Liu J, Zhu WM (2012) Study on a hydrogen peroxide biosensor based on horseradish peroxidase/GNPs–thionine/chitosan. Electrochim Acta 62:327–33410.1016/j.electacta.2011.12.034 ↗
22. Golami-Borujeni F, Faramarzi MA, Nejatzadeh-Barandozi F, Mahvi AH (2013) Oxidative degradation and detoxification of textile azo dye by horseradish peroxidase enzyme. Fresenius Environ Bull 22:739–744
23. Sadhanandam P, Anumary A, Ashokkumar M, Thanikaivelan P (2013) Probing horseradish peroxidase catalyzed degradation of azo dye from tannery wastewater. SpringerPlus 2(1):1–810.1186/2193-1801-2-1 ↗
24. Zhou Y, Zhou T, Zhou R, Hu Y (2014) Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes. Luminescence 29:338–34310.1002/bio.2549 ↗
25. Kamada K, Tsukahara S, Soh N (2010) Magnetically applicable layered iron–titanate intercalated with biomolecules. J Mater Chem 20:5646–565010.1039/c0jm00173b ↗
26. Tsukahara S, Soh N, Kamada K (2012) Soft surface modification of layered titanate for biorecognition. J Phys Chem C 116:19285–1928910.1021/jp304107b ↗
27. Kamada K, Nakamura T, Tsukahara S (2011) Photoswitching of enzyme activity of horseradish peroxidase intercalated into semiconducting layers. Chem Mater 23:2968–297210.1021/cm200672f ↗
28. Kamada K, Tsukahara S, Soh N (2011) Enhanced ultraviolet light tolerance of peroxidase intercalated into titanate layers. J Phys Chem C 115:13232–1323510.1021/jp203332v ↗
29. Ohya T, Nakayama A, Ban T, Ohya Y, Takahashi Y (2002) Synthesis and characterization of halogen-free, transparent, aqueous colloidal titanate solutions from titanium alkoxide. Chem Mater 14:3082–308910.1021/cm0200588 ↗
30. Schild HG (1992) Poly(N-isopropylacrylamide): experiment, theory and application. Prog Polym Sci 17:163–24910.1016/0079-6700(92)90023-R ↗