3D printed polyetheretherketone bone tissue substitute modified via amoxicillin-laden hydroxyapatite nanocoating
Source abstract
Abstract
A hydroxyapatite (HA) nano-coated polyetheretherketone (PEEK) bone tissue scaffold modified with amoxicillin (AMX) was developed by 3D printing, modified hydrothermal method and impregnation. The morphology of the HA coating and the effect of the coating on mechanical properties, cytocompatibility and antibacterial properties were then investigated. Results found that the morphology of HA coating on the PEEK scaffold could be controlled by adjusting reaction temperature or/and time. Moreover, the HA coatings obtained by reacting at 120 °C, pH 12 for 12 h, were the ordered arrangement of nanorods, similar to the ordered arrangement of HA in natural bone tissue. Mechanical testing results demonstrated no adverse effect of coating and modified process on the inherent mechanical properties of PEEK. Moreover, the compressive yield strength and Young`s modulus of the scaffolds were 11.23 ± 0.88 and 129.18 ± 8.10 Mpa, which could meet the mechanical requirements of native bone tissue. The cytocompatibility experiment with rabbit adipose-derived stem cells performed that the fabricated process and scaffolds were non-toxic, and cellular adhesion ability was enhanced after modification. The antibacterial properties of the AMX-modified scaffold were evaluated, and results confirmed that AMX could be loaded on the scaffold and released during cultivation to show excellent antibacterial properties. In conclusion, the fabricated modified HA-coated PEEK scaffold with AMX has great potential as a bone substitute in clinical application.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Dental materials and restorations · Orthopaedic implants and arthroplasty
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (59)
Show source-provided references
1. Kurtz SM, Devine JN (2007) PEEK biomaterials in trauma, orthopedic, and spinal implants. Biomaterials 28:4845–4869. https://doi.org/10.1016/j.biomaterials.2007.07.01310.1016/j.biomaterials.2007.07.013 ↗
2. Elhattab K, Sikder P, Walker JM, Bottino MC, Bhaduri SB (2020) Fabrication and evaluation of 3-D printed PEEK scaffolds containing macropores by design. Mater Lett 263. https://doi.org/10.1016/j.matlet.2019.12722710.1016/j.matlet.2019.127227 ↗
3. Ulery BD, Nair LS, Laurencin CT (2011) Biomedical applications of biodegradable polymers. J Polym Sci B Polym Phys 49:832–864. https://doi.org/10.1002/polb.2225910.1002/polb.22259 ↗
4. Wei Q, Deng NN, Guo J, Deng J (2018) Synthetic polymers for biomedical applications. Int J Biomater 2018:7158621. https://doi.org/10.1155/2018/715862110.1155/2018/7158621 ↗
5. Shi C, Yuan Z, Han F, Zhu C, Li B (2016) Polymeric biomaterials for bone regeneration. Ann Joint 1: 27–27. https://doi.org/10.21037/aoj.2016.11.0210.21037/aoj.2016.11.02 ↗
6. Meng YZ, Tjong SC, Hay AS, Wang SJ (2001) Synthesis and proton conductivities of phosphonic acid containing poly-(arylene ether)s. J Polymer Sci A Polymer Chem 39:3218–3226. https://doi.org/10.1002/pola.130410.1002/pola.1304 ↗
7. Du LC, Meng YZ, Wang SJ, Tjong SC (2004) Synthesis and degradation behavior of poly(propylene carbonate) derived from carbon dioxide and propylene oxide. J Appl Polymer Sci 92:1840–1846. https://doi.org/10.1002/app.2016510.1002/app.20165 ↗
8. Meng YZ, Tjong SC (1998) Rheology and morphology of compatibilized polyamide 6 blends containing liquid crystalline copolyesters. Polymer 39:99–107. https://doi.org/10.1016/s0032-3861(97)00218-810.1016/s0032-3861(97)00218-8 ↗
9. Rao PJ, Pelletier MH, Walsh WR, Mobbs RJ (2014) Spine interbody implants: material selection and modification, functionalization and bioactivation of surfaces to improve osseointegration. Orthop Surg 6:81–89. https://doi.org/10.1111/os.1209810.1111/os.12098 ↗
10. Heary RF, Parvathreddy N, Sampath S, Agarwal N (2017) Elastic modulus in the selection of interbody implants. J Spine Surg 3: 163–167. https://doi.org/10.21037/jss.2017.05.0110.21037/jss.2017.05.01 ↗
11. Okazaki Y, Gotoh E (2005) Comparison of metal release from various metallic biomaterials in vitro. Biomaterials 26:11–21. https://doi.org/10.1016/j.biomaterials.2004.02.00510.1016/j.biomaterials.2004.02.005 ↗
12. Scharf B, Clement CC, Zolla V, Perino G, Yan B, Elci SG, Purdue E, Goldring S, Macaluso F, Cobelli N, Vachet RW, Santambrogio L (2014) Molecular analysis of chromium and cobalt-related toxicity. Sci Rep 4:5729. https://doi.org/10.1038/srep0572910.1038/srep05729 ↗
13. Díez-Pascual AM, Naffakh M, Marco C, Ellis G, Gómez-Fatou MA (2012) High-performance nanocomposites based on polyetherketones. Progress Mater Sci 57:1106–1190. https://doi.org/10.1016/j.pmatsci.2012.03.00310.1016/j.pmatsci.2012.03.003 ↗
14. Haleem A, Javaid M (2019) Polyether ether ketone (PEEK) and its 3D printed implants applications in medical field: an overview. Clin Epidemiol Global Health 7:571–577. https://doi.org/10.1016/j.cegh.2019.01.00310.1016/j.cegh.2019.01.003 ↗
15. Haleem A, Javaid M, Khan RH, Suman R (2020) 3D printing applications in bone tissue engineering. J Clin Orthop Trauma 11:S118–S124. https://doi.org/10.1016/j.jcot.2019.12.00210.1016/j.jcot.2019.12.002 ↗
16. Javaid M, Haleem A (2020) 3D printed tissue and organ using additive manufacturing: An overview. Clin Epidemiol Global Health 8:586–594. https://doi.org/10.1016/j.cegh.2019.12.00810.1016/j.cegh.2019.12.008 ↗
17. Duncan JW, Bailey RA (2013) An analysis of fusion cage migration in unilateral and bilateral fixation with transforaminal lumbar interbody fusion. Eur Spine J 22:439–445. https://doi.org/10.1007/s00586-012-2458-x10.1007/s00586-012-2458-x ↗
18. Molino G, Palmieri MC, Montalbano G, Fiorilli S, Vitale-Brovarone C (2020) Biomimetic and mesoporous nano-hydroxyapatite for bone tissue application: a short review. Biomed Mater 15:022001. https://doi.org/10.1088/1748-605X/ab5f1a10.1088/1748-605X/ab5f1a ↗
19. Lowe B, Hardy JG, Walsh LJ (2020) Optimizing nanohydroxyapatite nanocomposites for bone tissue engineering. ACS Omega 5:1–9. https://doi.org/10.1021/acsomega.9b0291710.1021/acsomega.9b02917 ↗
20. Turon P, del Valle L, Alemán C, Puiggalí J (2017) Biodegradable and biocompatible systems based on hydroxyapatite nanoparticles. Appl Sci 7. https://doi.org/10.3390/app701006010.3390/app7010060 ↗
21. Xue C, Chen Y, Huang Y, Zhu P (2015) Hydrothermal synthesis and biocompatibility study of highly crystalline carbonated hydroxyapatite nanorods. Nanoscale Res Lett 10:1018. https://doi.org/10.1186/s11671-015-1018-910.1186/s11671-015-1018-9 ↗
22. Li K, Tjong SC (2011) Hydrothermal synthesis and biocompatibility of hydroxyapatite nanorods. J Nanosci Nanotechnol 11:10444–10448. https://doi.org/10.1166/jnn.2011.395010.1166/jnn.2011.3950 ↗
23. Li K, Yeung CY, Yeung KWK, Tjong SC (2012) Sintered hydroxyapatite/polyetheretherketone nanocomposites: mechanical behavior and biocompatibility. Adv Eng Mater 14:B155–B165. https://doi.org/10.1002/adem.20108014510.1002/adem.201080145 ↗
24. Liao CZ, Wong HM, Yeung KW, Tjong SC (2014) The development, fabrication, and material characterization of polypropylene composites reinforced with carbon nanofiber and hydroxyapatite nanorod hybrid fillers. Int J Nanomed 9:1299–1310. https://doi.org/10.2147/IJN.S5833210.2147/IJN.S58332 ↗
25. Chan KW, Liao CZ, Wong HM, Kwok Yeung KW, Tjong SC (2016) Preparation of polyetheretherketone composites with nanohydroxyapatite rods and carbon nanofibers having high strength, good biocompatibility and excellent thermal stability. RSC Adv 6:19417–19429. https://doi.org/10.1039/c5ra22134j10.1039/c5ra22134j ↗
26. Liu C, Chan KW, Shen J, Liao CZ, Yeung KWK, Tjong SC (2016) Polyetheretherketone hybrid composites with bioactive nanohydroxyapatite and multiwalled carbon nanotube fillers. Polymers (Basel) 8. https://doi.org/10.3390/polym812042510.3390/polym8120425 ↗
27. Salmasi S, Nayyer L, Seifalian AM, Blunn GW (2016) Nanohydroxyapatite effect on the degradation, osteoconduction and mechanical properties of polymeric bone tissue engineered scaffolds. Open Orthop J 10:900–919. https://doi.org/10.2174/187432500161001090010.2174/1874325001610010900 ↗
28. Guillaume O, Geven MA, Sprecher CM, Stadelmann VA, Grijpma DW, Tang TT, Qin L, Lai Y, Alini M, de Bruijn JD, Yuan H, Richards RG, Eglin D (2017) Surface-enrichment with hydroxyapatite nanoparticles in stereolithography-fabricated composite polymer scaffolds promotes bone repair. Acta Biomater 54:386–398. https://doi.org/10.1016/j.actbio.2017.03.00610.1016/j.actbio.2017.03.006 ↗
29. Shalumon KT, Kuo CY, Wong CB, Chien YM, Chen HA, Chen JP (2018) Gelatin/nanohyroxyapatite cryogel embedded poly(lactic-co-glycolic acid)/nanohydroxyapatite microsphere hybrid scaffolds for simultaneous bone regeneration and load-bearing. Polymers (Basel) 10. https://doi.org/10.3390/polym1006062010.3390/polym10060620 ↗
30. Li L, Song K, Chen Y, Wang Y, Shi F, Nie Y, Liu T (2018) Design and Biophysical Characterization of Poly (l-Lactic) Acid microcarriers with and without modification of chitosan and nanohydroxyapatite. Polymers (Basel), 10. https://doi.org/10.3390/polym1010106110.3390/polym10101061 ↗
31. Catalan KN, Corrales TP, Forero JC, Romero CP, Acevedo CA (2019) Glass transition in crosslinked nanocomposite scaffolds of gelatin/chitosan/hydroxyapatite. Polymers (Basel),11. https://doi.org/10.3390/polym1104064210.3390/polym11040642 ↗
32. Liu Y, Gu J, Fan D (2020) Fabrication of high-strength and porous hybrid scaffolds based on nano-hydroxyapatite and human-like collagen for bone tissue regeneration. Polymers (Basel), 12. https://doi.org/10.3390/polym1201006110.3390/polym12010061 ↗
33. Abu Bakar MS, Cheng MHW, Tang SM, Yu SC, Liao K, Tan CT, Khor KA, Cheang P (2003) Tensile properties, tension–tension fatigue and biological response of polyetheretherketone–hydroxyapatite composites for load-bearing orthopedic implants. Biomaterials 24:2245–2250. https://doi.org/10.1016/s0142-9612(03)00028-010.1016/s0142-9612(03)00028-0 ↗
34. Converse GL, Yue W, Roeder RK (2007) Processing and tensile properties of hydroxyapatite-whisker-reinforced polyetheretherketone. Biomaterials 28:927–935. https://doi.org/10.1016/j.biomaterials.2006.10.03110.1016/j.biomaterials.2006.10.031 ↗
35. Lee JH, Jang HL, Lee KM, Baek HR, Jin K, Hong KS, Noh JH, Lee HK (2013) In vitro and in vivo evaluation of the bioactivity of hydroxyapatite-coated polyetheretherketone biocomposites created by cold spray technology. Acta Biomater 9:6177–6187. https://doi.org/10.1016/j.actbio.2012.11.03010.1016/j.actbio.2012.11.030 ↗
36. Shimizu T, Fujibayashi S, Yamaguchi S, Otsuki B, Okuzu Y, Matsushita T, Kokubo T, Matsuda S (2017) In vivo experimental study of anterior cervical fusion using bioactive polyetheretherketone in a canine model. PLoS ONE 12:e0184495. https://doi.org/10.1371/journal.pone.018449510.1371/journal.pone.0184495 ↗
37. Liu Y, Rath B, Tingart M, Eschweiler J (2020) Role of implants surface modification in osseointegration: a systematic review. J Biomed Mater Res A 108:470–484. https://doi.org/10.1002/jbm.a.3682910.1002/jbm.a.36829 ↗
38. Ma R, Tang T (2014) Current strategies to improve the bioactivity of PEEK. Int J Mol Sci 15:5426–5445. https://doi.org/10.3390/ijms1504542610.3390/ijms15045426 ↗
39. Barkarmo S, Wennerberg A, Hoffman M, Kjellin P, Breding K, Handa P, Stenport V (2013) Nano-hydroxyapatite-coated PEEK implants: a pilot study in rabbit bone. J Biomed Mater Res A 101:465–471. https://doi.org/10.1002/jbm.a.3435810.1002/jbm.a.34358 ↗
40. Johansson P, Jimbo R, Naito Y, Kjellin P, Currie F, Wennerberg A (2016) Polyether ether ketone implants achieve increased bone fusion when coated with nano-sized hydroxyapatite: a histomorphometric study in rabbit bone. Int J Nanomed 11:1435–1442. https://doi.org/10.2147/IJN.S10042410.2147/IJN.S100424 ↗
41. Johansson P, Jimbo R, Kozai Y, Sakurai T, Kjellin P, Currie F, Wennerberg A (2015) Nanosized hydroxyapatite coating on PEEK implants enhances early bone formation: a histological and three-dimensional investigation in rabbit bone. Materials (Basel) 8:3815–3830. https://doi.org/10.3390/ma807381510.3390/ma8073815 ↗
42. Lee JH, Jang HL, Lee KM, Baek HR, Jin K, Noh JH (2017) Cold-spray coating of hydroxyapatite on a three-dimensional polyetheretherketone implant and its biocompatibility evaluated by in vitro and in vivo minipig model. J Biomed Mater Res B Appl Biomater 105:647–657. https://doi.org/10.1002/jbm.b.3358910.1002/jbm.b.33589 ↗
43. Huang Y, Zhang X, Zhao R, Mao H, Yan Y, Pang X (2014) Antibacterial efficacy, corrosion resistance, and cytotoxicity studies of copper-substituted carbonated hydroxyapatite coating on titanium substrate. J Mater Sci 50:1688–1700. https://doi.org/10.1007/s10853-014-8730-110.1007/s10853-014-8730-1 ↗
44. Kolanthai E, Ganesan K, Epple M, Kalkura SN (2016) Synthesis of nanosized hydroxyapatite/agarose powders for bone filler and drug delivery application. Mater Today Commun 8:31–40. https://doi.org/10.1016/j.mtcomm.2016.03.00810.1016/j.mtcomm.2016.03.008 ↗
45. Yang C, Huan Z, Wang X, Wu C, Chang J (2018) 3D Printed Fe Scaffolds with HA nanocoating for bone regeneration. ACS Biomater Sci Eng 4:608–616. https://doi.org/10.1021/acsbiomaterials.7b0088510.1021/acsbiomaterials.7b00885 ↗
46. Hu Q, Zhang R, Zhang H, Yang D, Liu S, Song Z, Gu Y, Ramalingam M (2021) Topological structure design and fabrication of biocompatible PLA/TPU/ADM mesh with appropriate elasticity for hernia repair. Macromol Biosci 21:e2000423. https://doi.org/10.1002/mabi.20200042310.1002/mabi.202000423 ↗
47. Liu S, Zhang H, Ahlfeld T, Kilian D, Liu Y, Gelinsky M, Hu Q (2022) Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-based multi-material hydrogel composites. Bio-Des Manufact. https://doi.org/10.1007/s42242-022-00194-310.1007/s42242-022-00194-3 ↗
48. Liu S, Hu Q, Shen Z, Krishnan S, Zhang H, Ramalingam M (2022) 3D printing of self-standing and vascular supportive multimaterial hydrogel structures for organ engineering. Biotechnol Bioeng 119:118–133. https://doi.org/10.1002/bit.2795410.1002/bit.27954 ↗
49. Liu S, Zhang H, Hu Q, Shen Z, Rana D, Ramalingam M (2020) Designing vascular supportive albumen-rich composite bioink for organ 3D printing. J Mech Behav Biomed Mater 104:10364210.1016/j.jmbbm.2020.103642 ↗
50. Liu S, Sun L, Zhang H, Hu Q, Wang Y, Ramalingam M (2021) High-resolution combinatorial 3D printing of gelatin-based biomimetic triple-layered conduits for nerve tissue engineering. Int J Biol Macromolecules 166:1280–129110.1016/j.ijbiomac.2020.11.010 ↗
51. Liu S, Zhang H, Hu Q, Zhang C, Li S, Wang B (2020) A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects. J Biomater Appl 34:1479–1493. https://doi.org/10.1177/088532822091055710.1177/0885328220910557 ↗
52. Wang X, Xu S, Zhou S, Xu W, Leary M, Choong P, Qian M, Brandt M, Xie YM (2016) Topological design and additive manufacturing of porous metals for bone scaffolds and orthopaedic implants: a review. Biomaterials 83:127–141. https://doi.org/10.1016/j.biomaterials.2016.01.01210.1016/j.biomaterials.2016.01.012 ↗
53. Ferraz MP, Mateus AY, Sousa JC, Monteiro FJ (2007) Nanohydroxyapatite microspheres as delivery system for antibiotics: release kinetics, antimicrobial activity, and interaction with osteoblasts. J Biomed Mater Res A 81:994–1004. https://doi.org/10.1002/jbm.a.3115110.1002/jbm.a.31151 ↗
54. Zheng F, Wang S, Wen S, Shen M, Zhu M, Shi X (2013) Characterization and antibacterial activity of amoxicillin-loaded electrospun nano-hydroxyapatite/poly(lactic-co-glycolic acid) composite nanofibers. Biomaterials 34:1402–1412. https://doi.org/10.1016/j.biomaterials.2012.10.07110.1016/j.biomaterials.2012.10.071 ↗
55. Zheng X, Huang M, Ding C (2000) Bond strength of plasma-sprayed hydroxyapatite/Ti composite coatings. Biomaterials 21:841–849. https://doi.org/10.1016/s0142-9612(99)00255-010.1016/s0142-9612(99)00255-0 ↗
56. Davies JE, Mendes VC, Ko JC, Ajami E (2014) Topographic scale-range synergy at the functional bone/implant interface. Biomaterials 35:25–35. https://doi.org/10.1016/j.biomaterials.2013.09.07210.1016/j.biomaterials.2013.09.072 ↗
57. Yu F, Lian R, Liu L, Liu T, Bi C, Hong K, Zhang S, Ren J, Wang H, Ouyang N (2022) Biomimetic hydroxyapatite nanorods promote bone regeneration via accelerating osteogenesis of BMSCs through t cell-derived IL-22. ACS Nano 16:755–77010.1021/acsnano.1c08281 ↗
58. von Knoch F, Jaquiery C, Kowalsky M, Schaeren S, Alabre C, Martin I, Rubash HE, Shanbhag AS (2005) Effects of bisphosphonates on proliferation and osteoblast differentiation of human bone marrow stromal cells. Biomaterials 26:6941–6949. https://doi.org/10.1016/j.biomaterials.2005.04.05910.1016/j.biomaterials.2005.04.059 ↗
59. Furtos G, Rivero G, Rapuntean S, Abraham GA (2017) Amoxicillin-loaded electrospun nanocomposite membranes for dental applications. J Biomed Mater Res B Appl Biomater 105:966–976. https://doi.org/10.1002/jbm.b.3362910.1002/jbm.b.33629 ↗